Biological and molecular characterization of silkworm strains from the Brazilian germplasm bank of Bombyx mori

Authors: N.C. Pereira, R.E.F. Munhoz, T.S. Bignotto, R. Bespalhuk, L.B. Garay, C.R.N. Saez, V.A. Fassina, A. Nembri and M.A. Fernandez

Brazil has only one public genetic pool of Bombyx mori strains, which was established in 2005 at Universidade Estadual de Maringá, Maringá, Paraná State. This genetic bank has been maintained, and the strains have been characterized using genetic and morphological tools. The quantitative and qualitative traits, directly or indirectly .. Read More»

Genet. Mol. Res. 12(2): 2013.June.28.1

DOI: 10.4238/2013.June.28.1

Experimental optimization of the number of blocks by means of algorithms parameterized by confidence interval in popcorn breeding

Authors: T.O.M. Paula, C.D. Marinho, A.T. Amaral J�ºnior, L.A. Peternelli and L.S.A. Gon�§alves

The objective of this study was to determine the optimal number of repetitions to be used in competition trials of popcorn traits related to production and quality, including grain yield and expansion capacity. The experiments were conducted in 3 environments representative of the north and northwest regions of the State of Rio de Janeiro with 10 Brazilia.. Read More»

Genet. Mol. Res. 12(2): 2013.June.27.2

DOI: 10.4238/2013.June.27.2

Morphophysiological changes in Malpighian tubule cells during Myrmeleon uniformis development (Neuroptera, Myrmeleontidae)

Authors: C.A. Pacheco and T.L. Silva

The land arthropod secretory system is formed by structures called Malpighian tubules. These tubules are extremely important in insects because they maintain osmoregulation. They are located in the body cavity and are either free or inserted in the rectum wall, forming an arrangement known as the cryptonephridial system. The Malpighian tubules may have mo.. Read More»

Genet. Mol. Res. 12(2): 2013.June.27.1

DOI: 10.4238/2013.June.27.1

Mutations in NPHS2 (podocin) in Mexican children with nephrotic syndrome who respond to standard steroid treatment

Authors: J.S. Carrasco-Miranda, R. Garcia-Alvarez, R.R. Sotelo-Mundo, O. Valenzuela, M.A. Islas-Osuna and N. Sotelo-Cruz

Human nephrotic syndrome has been related to mutations in glomerular proteins. Mutations in the NPHS2 gene that encodes podocin have been described as responsible for steroid-resistant nephrotic syndrome. It has been advised to test for NPHS2 mutations in parallel or before giving steroid treatment in nephrotic syndrome patients in order to avoid unnecess.. Read More»

Genet. Mol. Res. 12(2): 2013.June.24.1

DOI: 10.4238/2013.June.24.1

Morphological and genetic variation of the pine shoot tunnel beetle Placusa pinearum (Staphylinidae) in China

Authors: J.-Y. Gao, B.-Z. Ji and S.-W. Liu

Placusa pinearum, a newly described beetle species found living in pine shoot tunnels bored by the pine tip moth, Dioryctria rubella (Pyralidae), has potential as a vector to transport biological agents for controlling this moth pest of pine trees because of the high degree of niche overlap (co-occurrence) between them. In order to help determine the suit.. Read More»

Genet. Mol. Res. 12(2):

Inactivation of putative PKS genes can double geldanamycin yield in Streptomyces hygroscopicus 17997

Authors: Y.H. Li, M. Li, W.Q. He, Y.G. Wang and R.G. Shao

The putative polyketide biosynthesis (PKS) genes cos10 and pg10 were inactivated by insertion of a kanamycin-resistance gene into the genome of the geldanamycin-producing strain, Streptomyces hygroscopicus 17997. The resultant inactivation were confirmed by PCR analysis. The abilities of the PKS gene inactivation strains to produce geldanamycin were compa.. Read More»

Genet. Mol. Res. 12(2): 2013.June.21.3

DOI: 10.4238/2013.June.21.3

Age-dependent expression of the BPI gene in Sutai piglets

Authors: J. Zhu, C. Zi, Z.C. Wu, L. Liu, X.R. Zheng, X.M. Su, G.Q. Zhu, X.G. Huang, S.L. Wu and W.B. Bao

We compared and analyzed expression of the BPI gene of Sutai piglets ranging from newborn to post-weaning days 8, 18, 30, and 35 by the real-time PCR method, in order to determine if it is involved in protection against disease caused by ETEC F18. There was a significant difference between 18- and 35-day expression in the jejunum. There were also signific.. Read More»

Genet. Mol. Res. 12(2): 2012.April.12.1

DOI: 10.4238/2012.April.12.1

Determination of genetic variation among chickpea genotypes and their F1 crosses with RAPD markers

Authors: K. Mahmood, W. Nazeer, M. Naeem, S. Freed, M.A. Khan, G. Abbas, M. Iqbal, A.R. Kulachi, S.A. Sajid, M.A. Sadiq and M. Saleem

The intensity of genetic diversity amongst chickpea genotypes and their crosses is unknown. The current study investigated the genetic diversity of chickpea genotypes and their F1 crosses by using random amplified polymorphic DNA (RAPD) markers. We assessed the variation among 6 chickpea genotypes and 15 F1 crosses with the RAPD markers. The 6 parents and.. Read More»

Genet. Mol. Res. 12(2): 2013.April.10.1

DOI: 10.4238/2013.April.10.1