Medical Genetics
Comparative analysis of genomic DNA extraction protocols: Maxi-preparation of quality DNA for genetic evaluation and phylogenetic studies
Authors: Muhammad Naeem, Shoaib Hassan, Samra Masud, Qurban Ali, Sikandar Hayat, Ammar Danyal Naeem and Zara Naeem
Four different DNA extraction protocols were compared for ability to produce DNA from the various tissues (caudal fin, pectoral fin, pelvic fin, muscle and gills) of three different freshwater fish species (Hypophthalmichthys molitrix, Labeo rohita and Sperata sarwari). Study describes the comparison of Phenol-chloroform; salt extraction.. Read More»
- Genet. Mol. Res. 19(1):
Expression of HCN and sodium channels in murine model of Type I diabetes
Authors: Anantha Maharasi Rama Krishnan and Kavitha Sankaranarayanan
Pancreatic apoptosis is an important event in the pathogenesis of Type 1 Diabetes. It is characterized by secretion and binding of inflammatory cytokines to the pancreatic islets, that activate downstream signaling mechanism. These signaling mechanisms finally increase intracellular calcium level and that lead to the activation of apoptotic pathways. Ion .. Read More»
- Genet. Mol. Res. 18(2):
Possible association of proinflammatory cytokine IL-19 gene polymorphism with psoriasis
Authors: Wani Aadil, Bashir Ahmad Ganai, Tahseena Akhtar Tarun Narang, Rajinder Kaur
Background: Interleukin (IL-19) a member of the IL-10 family involved in abnormal keratinocyte differentiation and proliferation which is the hall mark of psoriasis. Aim: The aim of this study was to investigate the association between IL-19 gene polymorphism in patients with chronic plaque psoriasis as a factor in the susceptibility and developme.. Read More»
- Genet. Mol. Res. 18(1):
Expression analysis of OIP5-AS1 in bladder tissues
Authors: Feraydoon Abdolmaleki, Soudeh Ghafoui-Fard, Mohammad Taheri, Alireza Mordadi, Mandana Afasharpad, Abolghasem Nikfallah, Bashir Nazparvar, Vahid Kholghi Oskooei, Mir Davood Omrani
Long non-coding RNAs (lncRNAs) have crucial roles in regulation of many aspects of cell functions which are related with carcinogenesis process. The lncRNA Opa Interacting Protein 5-antisense 1 (OIP5-AS1) is among lncRNAs whose roles in some human malignancies have been elucidated. In the current study, we aimed at identification of the role of this lncRN.. Read More»
- Genet. Mol. Res. 18(1):
The clinical significance of miR-7-5p downregulation in gastric dysplasia and gastric cancer tissue
Authors: Sara Omrani, Ali Zare, Shahriar Tarighi, Hamid Ghaedi, Mir Davood Omrani
miRNAs have been involved in the pathogenesis of several cancers such as gastric cancer (GC.). In the following study we analyzed the expression of miR-7 in gastric cancer samples compared non-tumor tissues and gastric dysplasia (GD) samples with patients’ clinico-pathological data and its potential as diagnostic biomarker. We first analyzed its exp.. Read More»
- Genet. Mol. Res. 18(4):
NNT and NNT-AS1 expression levels are not different between bipolar patients and healthy subjects
Authors: Soudeh Ghafouri-Fard, Mohammad Taheri, Vahid Kholghi Oskooie, Mir Davood Omrani, Arezou Sayad
Nicotinamide nucleotide transhydrogenase (NNT) participates in redox and energy interconnections. Nnt silencing in N27 dopaminergic cells has changed basal, extra, and greatest mitochondrial oxygen absorption rates and made these cell vulnerable to constant upsurges in H2O2. NNT-antisense RNA1 (NNT-AS1) is transcribed from the opposite stand of NNT gene a.. Read More»
- Genet. Mol. Res. 18(1):
Relationship between melanoma-associated antigen 1 (MAGE-A1) gene polymorphisms and colorectal cancer development
Authors: Mikhlid Almutairi and Abdelhabib Semlali
In the Saudi population, colorectal cancer (CRC) is the first and third most common malignancy among males and females, respectively. The CRC risk is associated with the expression of certain genes, which can be used for prognoses and for finding screening-related biomarkers. One such gene is melanoma-associated antigen 1 (MAGE-A1); however, little is kno.. Read More»
- Genet. Mol. Res. 18(4):
Investigation of the role of apoptosis, necroptosis and DNA damage signaling pathways in multiple sclerosis
Authors: Romina Dastmalchi, Arezou Sayad, Mir Davood Omrani, Soudeh Ghafouri-Fard, Shahram Arsang-Jang, Mehrdokht Mazdeh, Mohammad Taheri*
Multiple sclerosis (MS) as the most common autoimmune disorder in the central nervous system is a major health problem. MS is a complex disease with multiple environmental and genetic factors participating in it. In the present study, we evaluated the expression of 3 genes named BCL2, CASP2 and CASP8 in peripheral blood of 50 MS patients and 50 healthy co.. Read More»
- Genet. Mol. Res. 17(4):
- http://dx.doi.org/gmr16039937
- DOI:
- http://dx.doi.org/10.4238/gmr16039937
Long non-coding RNA-protein interaction: the preliminary step to track their biological functions
Authors: Amr. R. Ghanam, Wafa Ali, Mohnad Abdalla
RNA is not only an intermediate for the flow of genetic information between DNA and protein. The transcription of the entire genomes generates a myriad non-protein coding RNA in addition to protein coding genes. Among these transcripts, lncRNA which is the least well understood and they cannot be considered as merely transcriptional noises. Here, we revie.. Read More»
- Genet. Mol. Res. 17(4):
- http://dx.doi.org/gmr16039920
- DOI:
- http://dx.doi.org/10.4238/gmr16039920
Anthocyanins-loaded Eudragit® L100 nanoparticles: in vitro cytotoxic and genotoxic analysis
Authors: TB de Queiroz, D Dupeyrón, JCT Carvalho, IOM Gaivão, and EL Maistro
Anthocyanins are flavonoids compounds that have a great therapeutic potential acting as antioxidants, anti-inflammatory and anti-carcinogenic agents. They are consumed through food, pharmaceutical and cosmetics industries as a natural alternative to synthetic dyes. However, anthocyanins are unstable depending on pH, temperatur.. Read More»
- Genet. Mol. Res. 17(1):
- gmr16039875
- DOI:
- 10.4238/gmr16039875