Table of contents: 2018 ( Volume 17, Issue 1 )
Animal Genetics   Research Article

Characterization of 33 microsatellite markers and development of multiplex PCR for yellow-throated marten (Martes flavigula)

The yellow-throated marten (Martes flavigula Boddaert 1785) is a medium-sized carnivore and a top predator in South Korea that is distributed throughout Western and Southeast Asia and Siberia in a wide range of habitats. In this study, we developed a panel of polymorphic microsatellite markers for M. flavigula by Illumina next-generation sequencing for investigation.. Read More»

Genet. Mol. Res. 17(1): gmr16039854

DOI: 10.4238/gmr16039854

Plant Genetics   Research Article

Cloning and expression analysis of pistillata-like gene in Sedirea japonica

Members of the pistillata-like (PI-like) subfamily of plant MADS-box genes play multiple roles in regulation of reproductive meristems and the specification of sepal and petal identities. However, the function of PI-like genes in the Sedirea japonica is largely unknown. In this study, a full-length of PI-like gene in Sedirea japonica (designated PI-like, GenBank No... Read More»

Genet. Mol. Res. 17(1): gmr16039855

DOI: 10.4238/gmr16039855

Plant Genetics   Research Article

Parapatric speciation found in Limnonectes (Dicroglossidae) species in Thailand

Biodiversity stems from speciation, and species identification allows scientists to measure species biodiversity. However, some closely related species, particularly amphibians that choose to inhabit the same environment, are difficult to identify. Juvenile specimens of Limnonectes gyldenstolpei (Lg) and L. taylori (Lt) were used as models to study the speciation pr.. Read More»

Genet. Mol. Res. 17(1): gmr16039856

DOI: 10.4238/gmr16039856

Microbial Genetics   Research Article

LC-MS based identification of secondary metabolites from marine antagonistic endophytic bacteria

Halophytes occupy coastal and sub-coastal area of marine environment. They potential candidates for search of novel and new bacterial flora that have immense potential to yield novel therapeutic agents. Six different endophytic bacteria have been isolated from pneumatophores and roots of three halophytes (Salsola imbricata, Avicennia marina and Haplopeplis perfoliat.. Read More»

Genet. Mol. Res. 17(1): gmr16039857

DOI: 10.4238/gmr16039857

Human Genetics   Research Article

A novel homozygous stop gain mutation in SLC12A3 gene cause Gitelman syndrome in Saudi consanguineous family

Gitelman syndrome (GS) is a genetic disorder that affects kidney and causes an imbalance of charged atoms (ions) in the body, including ions of potassium, magnesium, and calcium. GS is characterized by hypokalemia and metabolic alkalosis. GS is a rare autosomal recessive renal tubulopathy disease caused by loss-of-function mutations in the SLC12A3 gene. Objective of.. Read More»

Genet. Mol. Res. 17(1): gmr16039858

DOI: 10.4238/gmr16039858

Animal Genetics   Research Article

Novel polymorphisms in bovine CD4 and LAG-3 genes associated with somatic cell counts of clinical mastitis cows

Clinical mastitis cows normally produce clotted milk, thus the much higher somatic cells in milk are unable to be counted by routine FOSS machine. The proteins coded by CD4 and LAG-3 genes can bind to MHC class II molecules and play important roles in in-flammatory diseases. The present study was designed to investigate the effects of single nucleotide polymorphisms.. Read More»

Genet. Mol. Res. 17(1): gmr16039859

DOI: 10.4238/gmr16039859

Human Genetics   Research Article

Biophysical and biochemical mechanisms disturbance of cellular genome leading to transmutation benign neoplasm into cancer

It was considered processes transmutation of genome mechanism from the point of view of thermodynamics, biophysics, and biochemistry. Just it was explained mechanisms maintenance stability Internal Energy of Stationary State an able-bodied organism according first law of thermodynamics. Besides it was considered some negative influences on an organism of solar radia.. Read More»

Genet. Mol. Res. 17(1): gmr16039860

DOI: 10.4238/gmr16039860

Plant Genetics   Research Article

Large-scale development, characterization, and cross-amplification of EST–SSR markers in Chinese chive

Chinese chive (Allium tuberosum Rottler ex Spr.) is an important vegetable crop. However, genetic and breeding studies of the species have been restricted by the lack of simple sequence repeat markers (SSRs). In the present study, a total of 2553 Chinese chive SSRs were developed from the species’ transcriptome, with 626, 643, and 536 of the makers located in .. Read More»

Genet. Mol. Res. 17(1): gmr16039861

DOI: 10.4238/gmr16039861

Plant Genetics   Research Article

Genetic diversity as tool to identify standard leaf nutrients in cassava genotypes

Among the factors affecting cassava yield, the leaf nutrients content has received little attention since cassava genotypes may grow well under inappropriate nutrient supply, however showing inadequate nutritional quality of tuberous roots. Considering this aspect, the genetic diversity should be explored in order to determine standard leaf nutrients in cassava geno.. Read More»

Genet. Mol. Res. 17(1): gmr16039862

DOI: 10.4238/gmr16039862

Plant Genetics   Research Article

Isolation and cross-amplification of fifteen microsatellites for oil-rewarding Calceolaria species (Calceolariaceae)

Calceolaria is a diversified American genus whose species are mainly visited by oil-collecting bees, representing a highly specialized plant-pollinator system. However, although this is a unique plant system, different aspects of its ecology and evolution remain unexplored. In this study we characterized fifteen polymorphic microsatellites for four Andean Calceolari.. Read More»

Genet. Mol. Res. 17(1): gmr16039863

DOI: 10.4238/gmr16039863

Plant Genetics   Research Article

Ectopic Expression of AtCIPK23 Enhances Drought Tolerance via Accumulating Less H2O2 in Transgenic Tobacco Plants

Drought is a major threat to agricultural crop growth and productivity. However, the molecular mechanism behind such effects remains largely unknown. Plant CBL (Calcineurin B-Like)-interacting protein kinases (CIPKs) are believed to play an important role in plant drought tolerance and signalling transduction. Here, we report the influence of a member of the CIPK fa.. Read More»

Genet. Mol. Res. 17(1): gmr16039864

DOI: 10.4238/gmr16039864

Plant Genetics   Research Article

Genetic diversity in natural populations of Stylosanthes scabra using ISSR markers

Genetic diversity is the basis for genetic improvement, providing genes for new cultivars. Stylosanthes scabra is a legume used in pastures, presenting economic importance in tropical and subtropical regions. Molecular markers are targeted in population studies, thus we evaluated the genetic diversity distribution of S. scabra in Brazilian semi-arid northeastern amo.. Read More»

Genet. Mol. Res. 17(1): gmr16039866

DOI: 10.4238/gmr16039866

Plant Genetics   Research Article

Characterization of aapP and nopP genes related to the biological nitrogen fixation efficiency with soybean in contrasting strains of Bradyrhizobium japonicum

Soybean (Glycine max) is considered one of the most economically important agricultural crops in Brazil. Due to the high protein content of its grains, this crop demands a greater amount of nitrogen (N) for production. In general, for soybean, nitrogen fertilization costs through chemical fertilizers are considered high. However, the biological nitrogen fixation (BN.. Read More»

Genet. Mol. Res. 17(1): gmr16039867

DOI: 10.4238/gmr16039867

Animal Genetics   Research Article

Effect of genetic polymorphism of αS1- casein gene on qualitative and quantitative milk traits in native Bulgarian Rhodopean cattle breed

Milk protein genetic polymorphisms of the genus Bos provoke a significant scientific interest, mainly associated with their evolution, population structure, breeding and hybridization. The aim of present study is to investigate the influence of the genetic variants of αS1- casein gene with respect to milk production and quality of native for Bulgaria cattle br.. Read More»

Genet. Mol. Res. 17(1): gmr16039868

DOI: 10.4238/gmr16039868

Plant Genetics   Research Article

Determination of the number of harvests to select elite sugarcane genotypes in NE Brazil

Sixty-eight sugarcane genotypes were evaluated to determine the number of harvests required to select new genotypes in the sugarcane belt in the state of Pernambuco, northeast Brazil. Using a random block design, the following parameters were evaluated: (i) polarizable sugars (POL) per hectare, in metric tons (TPH); (ii) culm productivity per hectare (TCH); (iii) fi.. Read More»

Genet. Mol. Res. 17(1): gmr16039869

DOI: 10.4238/gmr16039869

Plant Genetics   Research Article

Genetic diversity assessment of Allium cepa L. cultivars from Bosnia and Herzegovina using SSR makers

The five most common cultivars from the genus Allium cepa L. in Bosnia and Herzegovina (BiH) were analysed, focusing on Konjic onion. Seven SSR markers for genetic similarity analysis were used to address the genetic backgrounds. The polymorphic relationship between the SSR markers was analyzed by using Polymorphic Information Content (PIC) and Heterozygosity (H) b.. Read More»

Genet. Mol. Res. 17(1): gmr16039870

DOI: 10.4238/gmr16039870

Plant Genetics   Research Article

Molecular Characterization and Differential Interaction of Xanthomonas axonopodis pv. phaseoli and Xanthomonas fuscans subsp. fuscans with Common Beans (Phaseolus vulgaris)

The common bacterial blight, caused by Xanthomonas axonopodis pv. phaseoli (Xap) and X. fuscans subsp. fuscans (Xff), is one of the most important dry bean diseases of bacterial etiology. The present work aimed to study the genetic structure among and within populations, and also identify the pathogenic variability of Xap and Xff isolates evaluating the horizontal a.. Read More»

Genet. Mol. Res. 17(1): gmr16039871

DOI: 10.4238/gmr16039871

Plant Genetics   Review Article

Hierarchical outcrossing among and within fruits in Bertholletia excelsa Bonpl. (Lecythidaceae) open-pollinated seeds

Knowing the mating patterns is important to determine the number of trees necessary for seed collection for conservation ex situ, tree breeding and environmental reforestation purposes. We investigated B. excelsa individuals and fruits, to check mating system index variations in a population by using open-pollinated seeds which were hierarchically sampled within and.. Read More»

Genet. Mol. Res. 17(1): gmr16039872

DOI: 10.4238/gmr16039872

Table of contents: 2018 ( Volume 17, Issue 2 )
Animal Genetics   Research Article

Correlation between qualitative indicators of Holstein sire′s semen

The differences of sperm performance parameters of sires related to their age and determined, regression analysis between the main quantitative and qualitative indicators of sperm performance of bulls of high producing dairy breeds and indicators of sperm was carried out. Analysis of indicators of sperm performance of sires of Holstein breed showed that with age the.. Read More»

Genet. Mol. Res. 17(2): http://dx.doi.org/gmr16039917

DOI: http://dx.doi.org/10.4238/gmr16039917

Human Genetics   Research Article

Null polymorphisms in GSTT1 and GSTM1 genes and their associations with smoking and cervical cancer

Human Papillomavirus (HPV) infection is the main risk factor for cervical cancer. However, other risk factors include smoking and genetic susceptibility. Glutathione-S-Transferases (GST) is enzymes involved in tobacco carcinogens metabolism and genes encoding these enzymes are highly polymorphic. The objective of this study was to compare the frequencies of GSTM1 an.. Read More»

Genet. Mol. Res. 17(2): http://dx.doi.org/gmr16039918

DOI: http://dx.doi.org/10.4238/gmr16039918

Plant Genetics   Research Article

Multivariate approach in Eucalyptus and its effects in the genotypes × environments interaction

The study aimed at the selection and evaluation of Eucalyptus spp. clones for the production of wood by means of multivariate statistical techniques. A total of 84 Eucalyptus clones were used belonging to the breeding program in Rio Grande do Sul, Brazil, in a randomized block design, with 20 replicates. The selection of clones from their values of genetic divergenc.. Read More»

Genet. Mol. Res. 17(2): http://dx.doi.org/gmr16039919

DOI: http://dx.doi.org/10.4238/gmr16039919

Table of contents: 2018 ( Volume 17, Issue 3 )
Animal Genetics   Research Article

Genetic and phenotypic association of the carnauba palm tree evaluated by inter-simple sequence repeat and biometric traits

The carnauba palm Copernicia prunifera is the third most important non-timber forest species in Brazil; it is mainly known for the production of carnauba wax. We examined intrapopulation genetic diversity and correlated genotypic and phenotypic traits of 28 trees from a coastal location in Rio Grande do Norte state. Phenotypic variables tree height, diameter at brea.. Read More»

Genet. Mol. Res. 17(3): http://dx.doi.org/gmr18018

DOI: http://dx.doi.org/10.4238/gmr18018

Animal Genetics   Research Article

Genetic diversity of a native population of Myrcia ovata (Myrtaceae) using ISSR molecular markers

Myrcia ovata Cambess. (Myrtaceae) is a medicinal and aromatic plant that has analgesic, bactericidal and fungicidal properties. Even though this plant has economic potential, nothing is known about the variability and genetic diversity of this species. This information is necessary to establish conservation strategies and allow prospection of natural resources. The .. Read More»

Genet. Mol. Res. 17(3): http://dx.doi.org/gmr18022

DOI: http://dx.doi.org/10.4238/gmr18022

Plant Genetics   Research Article

Multivariate approach in eucalyptus breeding and its effecton genotype x environment interactions

In view of the need to obtain genetically superior eucalyptus clones that promote productivity coupled with quality, we used multivariate statistical techniques for the selection and evaluation of Eucalyptus spp. clones for wood production. The experiments were carried out in three environments in the districts of Eldorado do Sul, Butiá and São Gabriel.. Read More»

Genet. Mol. Res. 17(3): http://dx.doi.org/gmr18025

DOI: http://dx.doi.org/10.4238/gmr18025

Human Genetics   Research Article

Phenotypic and predicted genetic approaches for genotype ranking of wheat seed yield in Brazil

This work was aimed at determining stability and adaptability through Additive Main Effects and Multiplicative Interaction (AMMI) and Genotype Main Effects and Genotype Environment Interaction (GGE) methodologies, as well as to estimate and predict Restricted Maximum Likelihood/Best Linear Unbiased Prediction (REML/BLUP) parameters and employ them in multivariate mo.. Read More»

Genet. Mol. Res. 17(3): http://dx.doi.org/gmr18026

DOI: http://dx.doi.org/10.4238/gmr18026

Human Genetics   Research Article

One-Step RT-qPCR assay for detection and quantification of equine infectious anemia virus in-vitro

Equine infectious anemia virus (EIAV) infection often results in an initial febrile response, followed by recurrent cycles of the disease and finally, a prolonged asymptomatic period. These variations in clinical signs are due to a number of factors, including virus strain, equid species and differences in susceptibility among animals. As a consequence of the close .. Read More»

Genet. Mol. Res. 17(3): http://dx.doi.org/gmr18027

DOI: http://dx.doi.org/10.4238/gmr18027

Human Genetics   Research Article

Detection of sequences related to biosynthesis of antifungal metabolites and identification of a PhzF-like gene in a Pseudomonas rhizobacterium of Piper tuberculatum

Our previous studies identified a Pseudomonas putida (Pt12 isolate) associated with roots of Piper tuberculatum Jacq., a Piperaceae occurring in the Amazon region with known resistance to infection by Fusarium solani f. sp. piperis, the causal agent of root rot disease in black pepper (Piper nigrum L.). This Pt12 isolate was able to inhibit in vitro growth of this f.. Read More»

Genet. Mol. Res. 17(3): http://dx.doi.org/gmr18035

DOI: http://dx.doi.org/10.4238/gmr18035

Plant Genetics   Research Article

Phenotypic stability of strawberry cultivars assessed in three environments

We examined the agronomic performance and stability of strawberry cultivars in three environments, using the Annicchiarico and AMMI methods. Thirteen strawberry cultivars were evaluated (Aromas, Oso Grande, Dover, Sweet Charlie, Tudla, Festival, Camarosa, Monterey, Palomar, Portola, Camino Real, San Andreas and Albion), in the towns of Lavras-MG, Guarapuava-PR and U.. Read More»

Genet. Mol. Res. 17(3): http://dx.doi.org/gmr18041

DOI: http://dx.doi.org/10.4238/gmr18041

Human Genetics   Research Article

High precision experimental statistics for the selection of common bean lines that have superior agronomic performance

The identification of a single statistic that allows selection of superior genotypes for several agronomic traits with high experimental precision would be useful for bean breeding programs. We examined correlations between 12 statistics for agronomic traits to determine which would be useful for the selection of common bean lines that have superior agronomic perfor.. Read More»

Genet. Mol. Res. 17(3): http://dx.doi.org/gmr18042

DOI: http://dx.doi.org/10.4238/gmr18042

Human Genetics   Research Article

Infertility caused by an association between Arg72Pro polymorphism of the p53 gene and Glu298Asp of the eNOS gene in patients with endometriosis

Endometriosis is characterized by ectopic endometrial tissue and affects millions of women worldwide. The disease leads to various symptoms such as chronic pelvic pain and infertility and does not yet have a well-defined etiology. The pathology is similar to cancer, since endometrial cells are highly proliferative, invade tissues and may be associated with tumor sup.. Read More»

Genet. Mol. Res. 17(3): http://dx.doi.org/gmr18046

DOI: http://dx.doi.org/10.4238/gmr18046

Human Genetics   Research Article

Effect of the PLAG1 gene polymorphism on oleic acid percentage in Japanese Black cattle populations

We investigated the effect of the PLAG1 gene (on bovine chromosome 14) polymorphism on oleic acid percentage (C18:1) in four Japanese Black cattle populations (JB1: n = 900, JB2: n = 560, JB3: n = 456, JB4: n = 450). We genotyped the PLAG1 polymorphism (rs109231213) for four populations and then analyzed the effects on carcass weight (CW) and C18:1. This polymorphis.. Read More»

Genet. Mol. Res. 17(3): http://dx.doi.org/gmr18056

DOI: http://dx.doi.org/10.4238/gmr18056

Human Genetics   Research Article

Genetic structure and diversity of Senefeldera verticillata (Euphorbiaceae) in semideciduous seasonal forest fragments

Senefeldera verticillata (Euphorbiaceae) is a species that exclusively occurs in the Atlantic Forest; it is used in the restoration and recovery of degraded areas and has therapeutic uses. Due to the scarcity of information on genetic patterns of this species, genetic diversity was characterized by the use of molecular markers, Inter Simple Sequence Repeats (ISSR). .. Read More»

Genet. Mol. Res. 17(3): http://dx.doi.org/gmr18059

DOI: http://dx.doi.org/10.4238/gmr18059

Plant Genetics   Research Article

Water stress adaptation of popcorn roots and association with agronomic traits

Water stress reduces the yield of agricultural crops severely. The objectives of this study were to investigate genotypic differences between 20 popcorn lines in environments under wellirrigated (WW) and water stressed (WS) conditions to determine phenotypic, genetic and environmental relations among agronomic and root traits. The experiment was arranged in a random.. Read More»

Genet. Mol. Res. 17(3): http://dx.doi.org/gmr18078

DOI: http://dx.doi.org/10.4238/gmr18078

Human Genetics   Research Article

CD40 rs4810485 T>G polymorphism and susceptibility to ankylosing spondylitis in the Latvian population

Ankylosing spondylitis (AS) is a potentially disabling form of a systemic chronic inflammatory arthritis affecting mainly the axial skeleton, with or without extraspinal manifestations. The genetic basis of AS is partly known. Moreover, many autoimmunityrelated genes have pleiotropic effects. Multiple functional polymorphisms in the genes encoding the tumor necrosis.. Read More»

Genet. Mol. Res. 17(3): http://dx.doi.org/gmr18081

DOI: http://dx.doi.org/10.4238/gmr18081

Human Genetics   Research Article

Selection of resistance sources to common bean anthracnose by field phenotyping and DNA marker-assisted screening

The main goal of this work was to select resistance sources to common bean anthracnose by field phenotyping and DNA marker-assisted screening. Fifty-five common bean genotypes, including differential varieties, characterized resistance sources, elite lines, cultivars and controls, were evaluated in a field inoculation trial and screened with SCAR markers linked to r.. Read More»

Genet. Mol. Res. 17(3): http://dx.doi.org/gmr18066

DOI: http://dx.doi.org/10.4238/gmr18066

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