Table of Contents | Genet. Mol. Res.2013(2)
Polymorphisms of the angiotensin II type 1 receptor gene affect antihypertensive response to angiotensin receptor blockers in hypertensive Chinese
Authors: H.T. Gong, X.L. Ma, B.X. Chen, X.Y. Xu, Q. Li, C.X. Guo and F.H. Du
The renin-angiotensin-aldosterone system plays a key role in regulating blood pressure by maintaining vascular tone and the water/sodium balance. Many antihypertensive drugs target the renin-angiotensin-aldosterone system, but the effect differs considerably among hypertensive patients. We investigated whether genetic variants of the angiotensin II type 1.. Read More»
Genet. Mol. Res. 12(2): 2013.June.21.2
DOI: 10.4238/2013.June.21.2
A new strategy for identification of currant (Ribes nigrum L.) cultivars using RAPD markers
Authors: J. Huo, G. Yang, Y. Zhang and F. Li
We developed a new approach using RAPD fingerprints to distinguish 37 currant cultivars from northeastern China based on optimization of RAPD by choosing 11 nucleotide primers and strict screening PCR annealing temperature. We found that the manual cultivar identification diagram (MCID) approach clearly developed fingerprints from 8 different primers that.. Read More»
Genet. Mol. Res. 12(2): 2013.June.21.1
DOI: 10.4238/2013.June.21.1
Genetic diversity of the honeybee Apis cerana in Yunnan, China, based on mitochondrial DNA
Authors: L. Yin and T. Ji
DNA sequence diversity in the tRNAleu-COII portion of the mitochondrial genome was investigated in samples of Apis cerana from Yunnan, China. A fragment of about 480 bp in tRNAleu-COII, including a noncoding area and part of COII, was sequenced. The noncoding area was 97-98 bp; 8 haplotypes were found, among which 5 had been reported previously, while 3 w.. Read More»
Genet. Mol. Res. 12(2): 2013.June.20.1
DOI: 10.4238/2013.June.20.1
Coexpression of interleukin-6 and -2 from giant panda in Escherichia coli and the biological activity of the fusion protein
Authors: Y. Yi, Y.-Y. Nian, H.-W. Ji, H. Zhang, L. Zhu and Z.-W. Xu
To construct a fusion cytokine protein with more and stronger bioactivities to enhance the immunity of the cytokine alone, we expressed interleukin (IL)-6/(IL)-2 from giant panda (Ailuropoda melanoleuca) in Escherichia coli as a 59.4-kDa fusion protein. Subsequently, the inclusion bodies were solubilized with 8 M urea and applied onto a Ni-nitrilotriaceti.. Read More»
Genet. Mol. Res. 12(2): 2013.June.14.1
DOI: 10.4238/2013.June.14.1
Persistent glucocorticoid resistance in systemic lupus erythematosus patients during clinical remission
Authors: A.K.G. Melo, M.R. Melo, A.B.A. Saramago, G. Demartino, B.D.B. Souza and C.A. Longui
Glucocorticoids (GCs) are key drugs in the treatment of systemic lupus erythematosus (SLE). GC dose reduction during remission is related to disease activity, GC dose used, length of treatment, and individual GC sensitivity. We compared GC receptor α (GRα) isoform and nuclear factor kappaB (NF-κB) messenger RNA quantitation and in vivo G.. Read More»
Genet. Mol. Res. 12(2): 2013.February.19.1
DOI: 10.4238/2013.February.19.1
Cobalt chloride induces metaphase when topically applied to larvae and pupae of the stingless bee Melipona scutellaris (Hymenoptera, Apidae, Meliponini)
Authors: C. Ueira-Vieira, R.R. Tavares, S. Morelli, B.B. Pereira, R.P. Silva, A.R. Torres-Mariano, W.E. Kerr and A.M. Bonetti
In order to optimize preparations of bee metaphases, we tested cobalt chloride, which has been used as a metaphase inducer in other organisms, such as hamsters and fish. Four microliters of 65 mM cobalt chloride aqueous solution was topically applied to larval and pupal stages of the stingless bee Melipona scutellaris. The cerebral ganglion was removed af.. Read More»
Genet. Mol. Res. 12(2): 2013.February.6.1
DOI: 10.4238/2013.February.6.1
Isolation and characterization of 16 microsatellite loci in marble goby (Oxyeleotris marmoratus)
Authors: J. Luo, X.P. Zhu, Y.H. Peng and S.W. Yin
A microsatellite-enriched genomic library for marble goby (Oxyeleotris marmoratus), a freshwater fish of considerable economic value, was obtained. A total of 16 microsatellite markers were successfully isolated and characterized in a population from the Mekong River in Vietnam. These markers had 2-20 alleles with expected heterozygosity ranging from 0.03.. Read More»
Genet. Mol. Res. 12(2): 2013.January.4.2
DOI: 10.4238/2013.January.4.2
Construction and validation of metagenomic DNA libraries from landfarm soil microorganisms
Authors: T.B.A. Pessoa, S.S. de Souza, A.F. Cerqueira, R.P. Rezende, C.P. Pirovani and J.C.T. Dias
Landfarming biodegradation is a strategy used by the petrochemical industry to reduce pollutants in petroleum-contaminated soil. We constructed 2 metagenomic libraries from landfarming soil in order to determine the pathway used for mineralization of benzene and to examine protein expression of the bacteria in these soils. The DNA of landfarm soil, collec.. Read More»
Genet. Mol. Res. 12(2): 2013.June.28.2
DOI: 10.4238/2013.June.28.2