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Bioinformatics   Research Article

Characterization of glucose-tolerant β-glucosidases used in biofuel production under the bioinformatics perspective: a systematic review

Authors: D.C.B. Mariano, C. Leite, L.H.S. Santos, L.F. Marins, K.S. Machado, A.V. Werhli, L.H.F. Lima and R.C. de Melo-Minardi

β-glucosidases are enzymes that catalyze the hydrolysis of oligosaccharides and disaccharides, such as cellobiose. These enzymes play a key role in cellulose degrading, such as alleviating product inhibition of cellulases. Consequently, they have been considered essential for the biofuel industry. However, the majority of.. Read More»

Genet. Mol. Res. 16(3):
gmr16039740
DOI:
10.4238/gmr16039740
Bioinformatics   Research Article

Increasing genetic variability in black oats using gamma irradiation

Authors: G. Silveira1, E. Moliterno2, G. Ribeiro3, P.M.A. Costa1, L.G. Woyann4, E.W. Tessmann4, A.C. Oliveira4 and C.D. Cruz1

The black oat (Avena strigosa Schreb) is commonly used for forage, soil cover, and green manure. Despite its importance, little improvement has been made to this species, leading to high levels of genotypic disuniformity within commercial cultivars. The objective of this study was to evaluate the efficiency of different doses of gamma rays [60Co] applied .. Read More»

Genet. Mol. Res. 13(4):
2014.December.4.28
DOI:
10.4238/2014.December.4.28
Bioinformatics   Research Article

Bioinformatic analysis of miRNA expression patterns in TFF2 knock-out mice

Authors: Y. Yin, H.Q. Shan, W. Huang, Y.M. Wu, H. Lu and Y. Jin

Trefoil factors, which bear a unique 3-loop trefoil domain, are a family of small secretory protease-resistant peptides (7-12 kDa) discovered in the 1980s. Trefoil factor 2 (TFF2) is a unique member of trefoil factors family that plays important roles in gastrointestinal mucosal defense and repair. However, few studies have characterized the miRNA express.. Read More»

Genet. Mol. Res. 13(4):
2014.October.20.26
DOI:
10.4238/2014.October.20.26
Bioinformatics   Research Article

Identification of marker genes in diabetic wounds by DNA microarray study

Authors: T. Ni1, N. Wang1, Z.G. Mao1 and M. Yao1,2

This study aimed to identify marker genes in diabetic wounds using a dataset based on a DNA microarray of dermal lymphatic endothelial cells, and our results provide a basic understanding of diabetic wounds through further study of these differentially expressed genes (DEGs). From the Gene Expression Omnibus database, we downloaded a gene expression micro.. Read More»

Genet. Mol. Res. 12(4):
DOI http://dx.doi.org/2013.November.7.9
DOI:
DOI http://dx.doi.org/10.4238/2013.November.7.9
Bioinformatics   Research Article

Identification of UV-B-induced microRNAs in wheat

Authors: B. Wang*, Y.F. Sun*, N. Song, X.J. Wang, H. Feng, L.L. Huang and Z.S. Kang

MicroRNAs (miRNAs) play critical roles in post-transcriptional gene regulation and act as important endogenous regulators to various stressors. Ultraviolet-B (UV-B) radiation is a major factor influencing crop growth and development. In this study, we isolated a novel wheat miRNA, named Tae-miR6000, and confirmed its expression diversity after UV-B treatm.. Read More»

Genet. Mol. Res. 12(4):
2013.October.7.7
DOI:
10.4238/2013.October.7.7
Bioinformatics   Research Article

Antioxidant capacity and meat quality of broilers exposed to different ambient humidity and ammonia concentrations

Authors: F.X. Wei, X.F. Hu, R.N. Sa, F.Z. Liu, S.Y. Li and Q.Y. Sun

To investigate the effect of humidity and ammonia on the antioxidative capacities and meat qualities of broilers, 192 broilers were divided into 2 groups: high (H, 70 ppm) and low (L, 30 ppm) ammonia concentration. These groups were divided into 30% (Treatment humidity, T) and 60% (Control humidity, C) humidity, giving 4 treat.. Read More»

Genet. Mol. Res. 13(2):
2014.April.17.8
DOI:
10.4238/2014.April.17.8
Bioinformatics   Research Article

Efficient detection of the V600E mutation of the BRAF gene in papillary thyroid carcinoma using multiplex allele-specific polymerase chain reaction combined with denaturing high-performance liquid chromatography

Authors: R.X. Gong1, Y.P. Gong1, J. Yang1, T. Wei1, J. Li-Ling2,3 and J.Q. Zhu1

Mutations in the V-raf murine sarcoma viral oncogene homolog B1 gene (BRAF) play an important role in the pathogenesis of papillary thyroid cancer (PTC). In this study, a BRAF V600E mutation was detected in formalin-fixed and paraffin-embedded PTC samples using multiplex allele-specific polymerase chain reaction and denaturing high-performance liquid chro.. Read More»

Genet. Mol. Res. 12(4):
2013.October.24.11
DOI:
10.4238/2013.October.24.11
Bioinformatics   Research Article

Effects of destrin pathway mutations on the gene expression profile

Authors: J.N. Xu, X. Liu, H. Wang, C.M. Hu, Q.H. Luo and Q.Q. Zhou

This study aimed to explore the interaction and crosstalk between pathways in response to destrin mutations. All the pathways from the MINT database were downloaded, a protein-protein interaction network was then constructed, and the crosstalk between pathways was investigated, in particular, the overlap of 2 significant pathw.. Read More»

Genet. Mol. Res. 13(2):
2014.April.8.5
DOI:
10.4238/2014.April.8.5
Bioinformatics   Research Article

An efficient algorithm for finding attractors in synchronous Boolean networks with biochemical applications

Authors: D. Zheng, G. Yang, X. Li, Z. Wang and W.N.N. Hung

Self-organized systems, genetic regulatory systems and other living systems can be modeled as synchronous Boolean networks with stable states, which are also called state-cycle attractors (SCAs). This paper summarizes three classes of SCAs and presents a new efficient binary decision diagram based algorithm to find all SCAs of synchronous Boolean networks.. Read More»

Genet. Mol. Res. 12(4):
2013.October.18.4
DOI:
10.4238/2013.October.18.4
Bioinformatics   Research Article

Variability analysis of ‘Persian’ acid lime tree selections using agronomic and molecular markers

Authors: M.G. Santos, O.S. Passos, W.S. Soares Filho, E.A. Girardi, A.S. Gesteira and C.F. Ferreira

‘Persian’ acid lime (PAL) is the most important triploid commercial citrus crop planted in the world. Little is known about the genetic variability of the selections used in Brazil. Therefore, 25 genotypes originating from the PAL, and three control species, Citrus sunki, C. limon, and C. aurantiifolia, were assessed using inter-simple sequenc.. Read More»

Genet. Mol. Res. 12(4):
2013.October.17.3
DOI:
10.4238/2013.October.17.3

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