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Bioinformatics

Bioinformatics   Research Article

Molecular phylogenetic study of Cardamine amaraeformis Nakai using nuclear and chloroplast DNA markers

Authors: M.A. Ali J. Lee S.Y. Kim F.M.A. Al-Hemaid

The internal transcribed spacer regions of nuclear ribosomal DNA, trnL and trnL-F chloroplast genes of Cardamine amaraeformis Nakai (Brassicaceae) were sequenced and analyzed with the sequences of related Cardamine sp available in GenBank to detect the pattern of C. amaraeformis evolutionary differentiation. C. amaraeformis, which is endemic to South Korea, .. Read More»

Genet. Mol. Res. 11(3):
2012.August.31.6
DOI:
10.4238/2012.August.31.6
Bioinformatics   Research Article

Patterns of genetic diversity and population structure of the threatened Houbara and Macqueen�s bustards as revealed by microsatellite markers

Authors: A. Korrida S. Jadallah F. Chbel A. Amin-Alami M. Ahra S. Aggrey

The Houbara bustard (Chlamydotis undulata) is a threatened avian species that is rapidly declining throughout its range, especially in North Africa, Asia and the Canary Islands. We examined the population structure and genetic variation for the three Houbara subspecies C. undulata undulata, C. u. fuertaventurae and C. u. macqueenii. A total of 266 birds from.. Read More»

Genet. Mol. Res. 11(3):
2012.September.12.4
DOI:
10.4238/2012.September.12.4
Bioinformatics   Research Article

Diallel cross analysis of plesiomorphic traits in Triticum aestivum L. genotypes

Authors: M. Shehzad, S.B. Hussain, M.K. Qureshi, M. Akbar, M. Javed1, H.M. Imran and S.A. Manzoor

We conducted a 5 x 5 complete diallel cross experiment in bread wheat (Triticum aestivum) with the genotypes 6309, Chkwal-50, Dhrabi, Bhkhar-02, and FS-08. Our objective was to evaluate the type of gene action and the general and specific combining abilities required for various morphological traits in wheat. The results of analysis of variance revealed h.. Read More»

Genet. Mol. Res. 14(4):
2015.October.28.9
DOI:
10.4238/2015.October.28.9
Bioinformatics   Research Article

Molecular characterization of sour orange (Citrus aurantium) accessions and their relatives using SSR and SRAP markers

Authors: I. Polat Y.A. Ka�§ar T. Yesiloglu A. Uzun O. Tuzcu O. Gulsen M. Incesu G. Kafa E. Turgutoglu S. Anil

Citrus production with its many varieties is of importance since it provides economically important products for Turkish exports. Sour orange is a rootstock commonly used for propagating the different scion varieties. Knowledge of the genetic diversity of the rootstock accessions would be useful in order to improve citrus breeding programs. We studied geneti.. Read More»

Genet. Mol. Res. 11(3):
2012.September.12.10
DOI:
10.4238/2012.September.12.10
Bioinformatics   Research Article

Interference on cytoplasmic polyadenylation element-binding proteins affects the invasion ability of glioma stem cells

Authors: H.L. Liu, J.F. Huo, Z.J. Liu and X.B. Chen

Glioma stem cells derived from primary cultures were divided into an experiment group, a control group, and a blank group and subjected to cytoplasmic polyadenilation element-binding protein (CPEBs) interference, transfection with empty vector, and normal culture, respectively, to compare their invasion abilities. Western blotting showed that siRNA-3 had .. Read More»

Genet. Mol. Res. 14(4):
2015.October.28.11
DOI:
10.4238/2015.October.28.11
Bioinformatics   Research Article

Identification of earl millet cultivars using both microsatellites and enzymatic markers

Authors: R.P. Mendon�§a Neto E.V.R. Von Pinho B.L. Carvalho G.S. Pereira

The increasing number of protected and registered cultivars and problems involving seed commercialization make distinction and identification of cultivars imperative. Millet (Pennisetum glaucum), a crop species with protected cultivars in Brazil, has been the target of seed piracy. Thus, with the objective of identifying different lots with regard to origin,.. Read More»

Genet. Mol. Res. 12(1):
2013.January.7.1
DOI:
10.4238/2013.January.7.1
Bioinformatics   Research Article

Genetic diversity in the germplasm of tropical maize landraces determined using molecular markers

Authors: D. Molin C.J. Coelho D.S. M�¡ximo F.S. Ferreira J.R. Gardingo R.R. Matiello

Maize landraces derived from tropical germplasm represent an important source of genetic variability, which is currently poorly understood and under-exploited by Brazilian crop breeding programs. The aims of our study were to a) estimate the genetic diversity across 48 varieties of maize landraces cultivated at different locations in the States of Rio Grande.. Read More»

Genet. Mol. Res. 12(1):
2013.January.22.8
DOI:
10.4238/2013.January.22.8
Bioinformatics   Research Article

An efficient approach to identify Ginkgo biloba cultivars by using random amplified polymorphic DNA markers with a manual cultivar identification diagram strategy

Authors: G.P. Li C.Q. Zhang F.L. Cao

Cultivar identification is a key step to avoid the formation of homonyms and synonyms of Ginkgo biloba. In this study, a new approach based on combinational utilization of polymorphic bands produced from 6 different random amplified polymorphic DNA (RAPD) primers was developed for identifying 42 Ginkgo cultivars, and a manual cultivar identification diagr.. Read More»

Genet. Mol. Res. 12(1):
2013.January.24.10
DOI:
10.4238/2013.January.24.10
Bioinformatics   Research Article

Analysis of genetic diversity in Larix gmelinii (Pinaceae) with RAPD and ISSR markers

Authors: L. Zhang H.G. Zhang X.F. Li

Dahurian larch (Larix gmelinii), a deciduous conifer, is the northernmost tree, native to eastern Siberia and nearby regions of China. We used growth traits and molecular markers to assess genetic variation in different L. gmelinii growing regions; 105 individual samples were collected from seven regions of the Qingshan Forestry Centre, Heilongjiang Province.. Read More»

Genet. Mol. Res. 12(1):
2013.January.24.12
DOI:
10.4238/2013.January.24.12
Bioinformatics   Research Article

Molecular cloning and characterization of GbDXS and GbGGPPS gene promoters from Ginkgo biloba

Authors: F. Xu X.H. Huang L.L. Li G. Deng H. Cheng X.F. Rong J.B. Li S.Y. Cheng

Ginkgolides are key pharmaceutical components in Ginkgo biloba leaves. 1-Deoxy-D-xylulose-5-phosphate synthase (GbDXS) and geranylgeranyl pyrophosphate (GbGGPPS) genes are critical genes involved in ginkgolide biosynthesis. In this study, the promoters of GbDXS and GGPPS, with 676 and 570 bp in length, respectively, were cloned by chromosome walking. The .. Read More»

Genet. Mol. Res. 12(1):
2013.February.4.3
DOI:
10.4238/2013.February.4.3