Table of Contents | Genet. Mol. Res.2013(2)
In silico analysis of diverse endophytic fungi by using ITS1-5,8S-ITS2 sequences with isolates from various plant families in Brazil
Authors: S.A. Rhoden, A. Garcia, J.L. Azevedo and J.A. Pamphile
Brazil has a great diversity of plants, and considering that all plant species studied to date have endophytic microorganisms (bacteria or fungi), the country is a resource in the search for bioactive compounds. Endophytes live within plants without causing damage and may be in dynamic equilibrium with the health of the plant. Endophytic fungi can be iden.. Read More»
Genet. Mol. Res. 12(2): 2013.April.2.10
DOI: 10.4238/2013.April.2.10
Complementary DNA cloning, sequence analysis, and tissue transcription profile of a novel U2AF2 gene from the Chinese Banna mini-pig inbred line
Authors: S.Y. Wang, J.L. Huo, Y.W. Miao, W.M. Cheng and Y.Z. Zeng
U2 small nuclear RNA auxiliary factor 2 (U2AF2) is an important gene for pre-messenger RNA splicing in higher eukaryotes. In this study, the Banna mini-pig inbred line (BMI) U2AF2 coding sequence (CDS) was cloned, sequenced, and characterized. The U2AF2 complete CDS was amplified using the reverse transcription-polymerase chain reaction (RT-PCR) technique.. Read More»
Genet. Mol. Res. 12(2): 2013.April.2.9
DOI: 10.4238/2013.April.2.9
Antimicrobial activity and cytotoxicity of endophytes from Scapania verrucosa Heeg.
Authors: J.-G. Wu, W. Peng, P.-Y. Zeng, Y.-B. Wu1, J. Yi and J.-Z. Wu
We evaluated the antibacterial activity and cytotoxicity of endophytes isolated from Scapania verrucosa Heeg., which belongs to the liverwort class. A total of 49 endophytic fungi were isolated from S. verrucosa and classified into seven genera and one family in our previous study. In this study, the cytotoxic activity of the endophytes was assessed using.. Read More»
Genet. Mol. Res. 12(2): 2013.April.2.8
DOI: 10.4238/2013.April.2.8
cDNA cloning and characterization of two trehalases from Spodoptera litura (Lepidoptera; Noctuidade)
Authors: Q. Zou, P. Wei, Q. Xu, H.Z. Zheng, B. Tang and S.G. Wang
The oriental leafworm moth, Spodoptera litura, is a major agricultural pest in southeast Asia and nearby Pacific regions. Two distinct trehalases have been identified in insects: soluble trehalase (Treh1) and membrane-bound trehalase (Treh2), although there is currently no information on these genes in S. litura. To characterize the distribution and funct.. Read More»
Genet. Mol. Res. 12(2): 2013.April.2.7
DOI: 10.4238/2013.April.2.7
Population structure of the blood clam (Tegillarca granosa) in China based on microsatellite markers
Authors: Y.J. Wang, Q.G. Zeng and L.N. Xu
The blood clam, Tegillarca granosa, is widely cultivated in China. We isolated 6 microsatellite loci from T. granosa and used them to investigate genetic diversity and population structure of 5 widely distributed populations of blood clam collected from eastern and southeastern China. The allele number per locus varied from 4 to 9, and the polymorphism in.. Read More»
Genet. Mol. Res. 12(2): 2013.April.2.6
DOI: 10.4238/2013.April.2.6
Genetic variability in Blanco Orejinegro breed cattle populations in Colombia
Authors: R. MartÃ?Ânez, A.P. LlinÃ?¡s and J.F. M.-Rocha
We analyzed population structure and genetic diversity in Blanco Orejinegro Creole cattle with 12 microsatellite markers, genotyping 138 individuals belonging to 7 commercial and 3 conservation herds. These markers showed a high level of polymorphism; 171 alleles were identified. The mean number of alleles per locus was 5.63 (3.82-6.58). The total number .. Read More»
Genet. Mol. Res. 12(2): 2013.April.10.4
DOI: 10.4238/2013.April.10.4
Inheritance of resistance to Pepper yellow mosaic virus in Capsicum baccatum var. pendulum
Authors: C.S. Bento, R. Rodrigues, L.S.A. Gon�§alves, H.S. Oliveira, M.H. Santos, M.C. Pontes and C.P. Sudr�©
We investigated inheritance of resistance to Pepper yellow mosaic virus (PepYMV) in Capsicum baccatum var. pendulum accessions UENF 1616 (susceptible) crossed with UENF 1732 (resistant). Plants from generations P1, P2, F1, F2, BC1:1, and BC1:2 were inoculated and the symptoms were evaluated for 25 days. Subsequently, an area under the disease progress cur.. Read More»
Genet. Mol. Res. 12(2): 2013.April.10.3
DOI: 10.4238/2013.April.10.3
Isolation and analysis of �±-expansin genes in the tree Anthocephalus chinensis (Rubiaceae)
Authors: K.X. Ouyang, M.Q. Liu, R.Q. Pian, S.S. Liu and X.Y. Chen
Expansins are cell wall-associated proteins that induce wall extension and relax stress by disrupting noncovalent bonds between cellulose microfibrils and cross-linking glycan chains, thereby promoting wall creep. Anthocephalus chinensis is a very fast-growing economically important tree found mainly in South Asia. Sixteen cDNAs, designated AcEXPA1 to AcE.. Read More»
Genet. Mol. Res. 12(2): 2013.April.10.2
DOI: 10.4238/2013.April.10.2