Table of Contents | Genet. Mol. Res.2013(2)
Construction of a cDNA library of the Chinese wild Vitis amurensis under cold stress and analysis of potential hardiness-related expressed sequence tags
Authors: J. Zhang, N. Liu, R. Niu, Y. Liu, H. Zhai, W. Xu and Y. Wang
A cDNA library of Chinese wild Vitis amurensis, which is the most cold-resistant species in the genus Vitis, was constructed using young leaves of seedlings of the clone Heilongjiang potted and subjected to cold stress. The leaves were harvested at various times after cold stress for total RNA extraction, which was used to generate expressed sequence tags.. Read More»
Genet. Mol. Res. 12(2): 2013.April.12.5
DOI: 10.4238/2013.April.12.5
Comparative multiplex dosage analysis in spinocerebellar ataxia type 2 patients
Authors: F. Cal�¬, V. Chiavetta, A. Ragalmuto, M. Vinci, G. Ruggeri, P. Schinocca and V. Romano
We developed a new application of comparative multiplex dosage analysis (CMDA) for evaluation of the ataxin 2 gene. Expansions of the triplet CAG can cause spinocerebellar ataxia type 2 (SCA2), a neurodegenerative disease with an autosomal-dominant mode of inheritance. Molecular diagnosis of SCA2 is routinely based on the use of conventional PCR to detect.. Read More»
Genet. Mol. Res. 12(2): 2013.April.12.4
DOI: 10.4238/2013.April.12.4
Mutational analyses of the TSC1 and TSC2 genes in cases of tuberous sclerosis complex in Chinese Han children
Authors: G.-X. Wang, D.-W. Wang, C.-Y. Yi, J.-S. Qu and Y.-L. Wang
Tuberous sclerosis complex (TSC) is an autosomal dominant neurogenetic disorder characterized by hamartomas in multiple organs and is caused by a wide spectrum of mutations in 1 of 2 causative genes (TSC1 or TSC2). Here, we present mutational analyses of the TSC1 and TSC2 genes in 4 cases of TSC in Chinese Han children, including 2 familial and 2 sporadic.. Read More»
Genet. Mol. Res. 12(2): 2013.April.12.3
DOI: 10.4238/2013.April.12.3
Genetic diversity analysis of Capsicum spp germplasm bank accessions based on �±/�²-esterase polymorphism
Authors: E.R. Monteiro, A.R. Bronzato, G.R. Orasmo, A.C.A. Lopes, R.L.F. Gomes, C.A. Mangolin and M.F.P.S. Machado
Genetic diversity and structure were analyzed in 10 accessions belonging to Banco Ativo de Germoplasma de Capsicum located at Federal University of Piauí in northwestern Brazil that receives pepper samples grown in community gardens in various regions and Brazilian states. Selections were made from seeds of C. chinense (4 accessions), C. annuum (5 .. Read More»
Genet. Mol. Res. 12(2): 2013.April.12.2
DOI: 10.4238/2013.April.12.2
Expression of glutamine synthetase in Tegillarca granosa (Bivalvia, Arcidae) hemocytes stimulated by Vibrio parahaemolyticus and lipopolysaccharides
Authors: Y.B. Bao, L. Li, M.X. Ye, Y.H. Dong, W.X. Jin and Z.H. Lin
The blood cockle, Tegillarca granosa, is a widely consumed clam in the Indo-Pacific region. Glutamine synthetase (GS) is an enzyme that plays an essential role in the metabolism of nitrogen by catalyzing the condensation of glutamate and ammonia to form glutamine. We identified the GS of T. granosa (Tg-GS) from hemocytes by 3'- and 5'-rapid amplification .. Read More»
Genet. Mol. Res. 12(2): 2013.April.10.9
DOI: 10.4238/2013.April.10.9
Evaluation of genotoxic and cytotoxic effects of antileishmanial extract from Julocroton triqueter (Euphorbiaceae)
Authors: V.R. Moreira, A.C.S. Dias, A.R.P. Martins, M.I.S. Lima, I.C.P.B. Serra, M.N.S. Ribeiro, F.R.F. Nascimento and S.R.F. Pereira
Julocroton triqueter extracts have antileishmanial activity; however, the effect on genetic stability has not been studied. We evaluated genotoxic and cell death induction potential (in vitro and in vivo) of J. triqueter var. triqueter hydroalcoholic extracts, as well as their antigenotoxic potential in vivo. The in vitro genotoxic studies were performed .. Read More»
Genet. Mol. Res. 12(2): 2013.April.10.8
DOI: 10.4238/2013.April.10.8
Genetic diversity analysis of the Uruguayan Creole cattle breed using microsatellites and mtDNA markers
Authors: E. Armstrong, A. Iriarte, A.M. MartÃ?Ânez, M. Feijoo, J.L. Vega-Pla, J.V. Delgado and A. Postiglioni
The Uruguayan Creole cattle population (N = 600) is located in a native habitat in south-east Uruguay. We analyzed its genetic diversity and compared it to other populations of American Creole cattle. A random sample of 64 animals was genotyped for a set of 17 microsatellite loci, and the D-loop hyper-variable region of mtDNA was sequenced for 28 calves o.. Read More»
Genet. Mol. Res. 12(2): 2013.April.10.7
DOI: 10.4238/2013.April.10.7
S-adenosylmethionine, a methyl donor, up regulates tissue inhibitor of metalloproteinase-2 in colorectal cancer
Authors: Z. Hussain, M.I. Khan, M. Shahid and F.N. Almajhdi
DNA methylation is a fundamental epigenetic mechanism in regulating the expression of genes controlling crucial cell functions in cancer development. Gene silencing via CpG island methylation/demethylation in the promoter region is one of the mechanisms by which different genes are inactivated/activated in human cancers. Tissue inhibitor of metalloprotein.. Read More»
Genet. Mol. Res. 12(2): 2013.April.10.6
DOI: 10.4238/2013.April.10.6