Table of Contents | Genet. Mol. Res.2013(2)
High levels of polymorphism found through cross-amplification of microsatellite loci in a Ctenomys pearsoni (Rodentia, Ctenomyidae) population
Authors: N. Mannise, S. Gonz�¡lez, J.E. Maldonado, G. Izquierdo, G. Francescoli and M. Cosse
Ctenomys pearsoni (Pearson’s tuco-tuco) is a subterranean rodent native to Uruguay. We tested the amplification pattern of 12 microsatellite loci, designed for C. sociabilis and C. haigi in a C. pearsoni population. DNA extractions were made from hair samples, and PCR amplification products were run on an ABI 3100 microcapillary gel. Eight loci were.. Read More»
Genet. Mol. Res. 12(2): 2013.April.2.5
DOI: 10.4238/2013.April.2.5
Gene expression study related with the intrinsic pathway of apoptosis in bladder cancer by real-time PCR technique
Authors: D.F. Barione, F.S.N. Lizarte, P.C. Novais, C.A.M. de Carvalho, F.C.B. Valeri, F.M. Peria, H.F. de Oliveira, D.L. Zanette, W.A. Silva Jr., A.J. Cologna, R.B. Reis, S. Tucci Jr., A.C.P. Martins, D.P.C. Tirapelli and L.F. Tirapelli
We examined the expression of anti-apoptotic genes (XIAP and Bcl-2) and apoptotic genes (cytochrome c, caspase-9, Apaf-1) in tissue samples of patients with superficial bladder cancer. Thirty-two bladder cancer tissue samples (8 papillary urothelial neoplasm of low malignant potential, 10 low-grade, and 14 high-grade) and 8 normal bladder tissue samples f.. Read More»
Genet. Mol. Res. 12(2): 2013.April.2.4
DOI: 10.4238/2013.April.2.4
Identification of sequence-related amplified polymorphism markers linked to the red leaf trait in ornamental kale (Brassica oleracea L. var. acephala)
Authors: Y.S. Wang, Z.Y. Liu, Y.F. Li, Y. Zhang, X.F. Yang and H. Feng
Artistic diversiform leaf color is an important agronomic trait that affects the market value of ornamental kale. In the present study, genetic analysis showed that a single-dominant gene, Re (red leaf), determines the red leaf trait in ornamental kale. An F2 population consisting of 500 individuals from the cross of a red leaf double-haploid line â.. Read More»
Genet. Mol. Res. 12(2): 2013.April.2.3
DOI: 10.4238/2013.April.2.3
Effect of two single nucleotide polymorphisms on milk yield and composition
Authors: J.C. Rinc�³n, A. L�³pez-Herrera and J.J. Echeverri
The bovine prolactin (PRL) and bovine growth hormone (bGH) genes exhibit several polymorphisms. Some of them can be detected by molecular techniques using restriction endonucleases, such as RsaI for the PRL gene and MspI for the bGH gene. We examined the relationship between the PRL-RsaI and bGH-MspI polymorphisms and some economically important character.. Read More»
Genet. Mol. Res. 12(2): 2013.April.2.15
DOI: 10.4238/2013.April.2.15
Apomixis in cassava: advances and challenges
Authors: D.Y.H. Freitas and N.M.A. Nassar
Cassava is the most important staple crop in the Tropics and Subtropics. Apomixis may revolutionize its production due to various attributes. These potential advantages include production by true seed, maintaining cultivar superiority over generations without segregation, and avoiding contamination by bacteria and viruses. Historically, apomixis was initi.. Read More»
Genet. Mol. Res. 12(2): 2013.April.2.14
DOI: 10.4238/2013.April.2.14
Genetic analysis of differentiation of T-helper lymphocytes
Authors: Q. Wang, M. Li, L.C. Xia, G. Wen, H. Zu and M. Gao
In the human immune system, T-helper cells are able to differentiate into two lymphocyte subsets: Th1 and Th2. The intracellular signaling pathways of differentiation form a dynamic regulation network by secreting distinctive types of cytokines, while differentiation is regulated by two major gene loci: T-bet and GATA-3. We developed a system dynamics mod.. Read More»
Genet. Mol. Res. 12(2): 2013.April.2.13
DOI: 10.4238/2013.April.2.13
Polymorphisms of the myostatin gene (MSTN) and its relationship with growth traits in goat breeds
Authors: Z.J. Zhang, Y.H. Ling, L.J. Wang, Y.F. Hang, X.F. Guo, Y.H. Zhang, J.P. Dingand X.R. Zhang
Mutations in the myostatin (MSTN) gene can inactivate its expression and result in a non-functional protein, which leads to dramatic muscularity and a “double-muscling” phenomenon in many species. Using gene sequencing and polymerase chain reaction-single-strand conformation polymorphism methods, polymorphisms of the MSTN gene were investigate.. Read More»
Genet. Mol. Res. 12(2): 2013.April.2.12
DOI: 10.4238/2013.April.2.12
Genetic dissection of agronomic traits within a segregating population of breeding table grapes
Authors: A.P. Viana, S. Riaz and M.A. Walker
Grapes (Vitis vinifera) are of great economic importance worldwide. We genetically dissected a table grape breeding population, using hidden Markov models (HMM) applied to quantitative trait locus (QTL) analyses. We evaluated and dissected the following traits: total number of clusters, leaf score, peduncle length, cluster length, number of berries, weigh.. Read More»
Genet. Mol. Res. 12(2): 2013.April.2.11
DOI: 10.4238/2013.April.2.11