Abstract

Determination of genetic variation among chickpea genotypes and their F1 crosses with RAPD markers

Author(s): K. Mahmood, W. Nazeer, M. Naeem, S. Freed, M.A. Khan, G. Abbas, M. Iqbal, A.R. Kulachi, S.A. Sajid, M.A. Sadiq and M. Saleem

The intensity of genetic diversity amongst chickpea genotypes and their crosses is unknown. The current study investigated the genetic diversity of chickpea genotypes and their F1 crosses by using random amplified polymorphic DNA (RAPD) markers. We assessed the variation among 6 chickpea genotypes and 15 F1 crosses with the RAPD markers. The 6 parents and their 21 hybrids were carefully studied based on the presence or absence of bands. The level of polymorphism varied with different primers. Of 28 primers used, 21 amplified the genomic DNA in all the varieties, 15 generated polymorphic bands among all the varieties, and 6 produced similar banding patterns.

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