Medical Genetics
Effect of RNAi-mediated silencing of Livin gene on biological properties of colon cancer cell line LoVo
Authors: A.M. Zou, H.F. Wang, W.F. Zhu, F.X. Wang and J.J. Shen
This study aimed to investigate the effect of RNAi-mediated silencing of the Livin gene on biological properties of the colon cancer cell line LoVo. Interference vectors pSilencer4.1-Ll and pSilencer4.1-L2 targeting the Livin gene were constructed and transfected into LoVo cells. The expression of the Livin gene was determined.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.16.8
- DOI:
- http://dx.doi.org/10.4238/2014.May.16.8
A multi-farm assessment of Greek black pig genetic diversity using microsatellite molecular markers
Authors: S. Michailidou, A. Kalivas, I. Ganopoulos, E. Stea, G. Michailidis, A. Tsaftaris and A. Argiriou
Local breeds are important for the maintenance of genetic diversity and future food security. Nowadays, the worldwide distribution of pigs is dominated by a few breeds, tending towards a severe loss of pig biodiversity. Thus, it is critical to maintain distinct populations of pig breeds. The Greek black pig, a breed raised loc.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.14.4
- DOI:
- 10.4238/2014.April.14.4
In vivo mechanism study of NGAL in rat renal ischemia-reperfusion injury
Authors: X.J. Zang1, S.X. An2, Z. Feng1, Y.P. Xia1, Y. Song and Q. Yu
This study aimed to determine the protective effect and mechanism of neutrophil gelatinase-associated lipocalin (NGAL) in rat kidney on ischemia/reperfusion injury (I/R). The rat I/R model was set up by cutting one kidney and clamping the contralateral renal pedicle for 45 min. Male SD rats were randomly divided into sham-oper.. Read More»
- Genet. Mol. Res. 13(4):
Isolation and characterization of microsatellites for the yam Dioscorea cayenensis (Dioscoreaceae) and cross-amplification in D. rotundata
Authors: L.R.G. Silva, M.M. Bajay, M. Monteiro, T.F. Mezette, W.F. Nascimento, M.I. Zucchi, J.B. Pinheiro and E.A. Veasey
Dioscorea cayenensis and Dioscorea rotundata are among the most important yam species for the humid and sub-humid tropics. We isolated nine polymorphic microsatellite markers using a microsatellite-enriched genomic library technique. The nine primer pairs were validated in 22 D. cayenensis accessions, and were tested for trans.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.14.5
- DOI:
- 10.4238/2014.April.14.5
Association of BRCA2 variants with cardiovascular disease in Saudi Arabia
Authors: M. Alanazi, N.P. Reddy, J.P. Shaik, S.A. Ajaj, A.A.A. Jafari, H. Saeed, Z. Khan and A.P. Khan
Abnormalities in the breast cancer tumor suppressor genes (BRCA1 and BRCA2) are associated with breast and ovarian cancer. Recently, two single nucleotide polymorphisms (SNPs; rs11571836 and rs1799943) were identified, both located in untranslated regions of chromosome 13, associated with cardiovascular disease (CVD) in a mult.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.16.13
- DOI:
- http://dx.doi.org/10.4238/2014.May.16.13
Association of NPP1 polymorphism with postoperative progression of ossification of the posterior longitudinal ligament in Chinese patients
Authors: Z. He, H. Zhu, L. Ding, H. Xiao, D. Chen and F. Xue
Ossification of the posterior longitudinal ligament (OPLL) is a condition of the spine that can cause paralysis by compressing the spinal cord. The aim of this study was to evaluate the possible role of nucleotide pyrophosphatase phosphodiesterase 1 gene (NPP1) polymorphism in the etiology and pathology of the OPLL in Chinese patients. DNA from patients w.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.18.3
- DOI:
- 10.4238/2013.October.18.3
Analysis of differentially expressed genes in malignant biliary strictures
Authors: D.C. Qi, B. Wu, S.L. Tao, J. Zhou, H.X. Qian and D. Wang
Microarray data were collected from bile duct samples from subjects with malignant biliary strictures by endoscopic retrograde cholangiopancreatography to screen for key genes associated with this disease. A predicted interaction network was constructed for these genes to interpret their functions. The gene expression dataset .. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.8.10
- DOI:
- 10.4238/2014.April.8.10
MicroRNA network analysis identifies key microRNAs and genes associated with precancerous lesions of gastric cancer
Authors: X.W. Wang1, Y. Wu2, D. Wang1 and Z.F. Qin
To identify potential targets for the early treatment and prevention of gastric cancer, microRNA (miRNA) expression profiles of precancerous lesions of gastric cancer were investigated. The miRNA microarray dataset GSE24839 was downloaded from Gene Expression Omnibus (GEO) and included 10 Helicobacter pylori-related gastritis samples and 10 gastric intest.. Read More»
- Genet. Mol. Res. 13(4):
Electrophoresis and spectrometric analyses of adaptation-related proteins in thermally stressed Chromobacterium violaceum
Authors: I.B. Cordeiro1, D.P. Castro7, P.P.O. Nogueira7, P.C.S. Angelo6, P.A. Nogueira7, J.F.C. Gon�§alves8, A.M.R.F. Pereira9, J.S. Garcia2,3, G.H.M.F. Souza2,4, M.A.Z. Arruda5, M.N. Eberlin2, S. Astolfi-Filho1, E.V. Andrade1 and J.L. L�³pez-Lozano10
Chromobacterium violaceum is a Gram-negative proteobacteria found in water and soil; it is widely distributed in tropical and subtropical regions, such as the Amazon rainforest. We examined protein expression changes that occur in C. violaceum at different growth temperatures using electrophoresis and mass spectrometry. The total number of spots detected .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.29.1
- DOI:
- 10.4238/2013.October.29.1
Effects of cisplatin and panobinostat on human mesothelial (Met-5A) and malignant pleural mesothelioma (MSTO-211H) cells
Authors: K.E. Gultekin1, M.K. Yurdakonar1, E. Yaman1, U.S. Yuce1, A. Yilmaz2, E. Alp3, A. Celik4, S.M. Demiroz4 and H.I. Onen2
New therapeutic approaches are still needed for effective malignant pleural mesothelioma treatment. The use of classical chemotherapy agents in combination with newly developed molecules may shed light on new therapeutic approaches. We aimed to determine the efficacy of panobinostat, alone and in combination with cisplatin, on cell survival and mRNA expre.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.11.2
- DOI:
- 10.4238/2013.November.11.2