Medical Genetics
Higher frequency of CD4+CD25high Treg cells in hemophilia patients with factor VIII inhibitor
Authors: K.Y. Ding, W.C. Ji, J.S. Wu, T. Li and Y.Y. Sheng
The production of factor VIII inhibitor antibodies remains the most costly and serious complication in replacement therapy of hemophilia A. We investigated the clinical significance of CD4+CD25high T regulatory (Treg) cells in hemophilia patients. Our trial included 6 severe hemophilia A patients with factor VIII inhibitors, 6.. Read More»
- Genet. Mol. Res. 13(1):
- http://dx.doi.org/2014.March.17.5
- DOI:
- http://dx.doi.org/10.4238/2014.March.17.5
Detecting the potential cancer association or metastasis by multi-omics data analysis
Authors: L. Hua, WY. Zheng, H. Xia and P. Zhou
Comprehensive multi-omics data analyses have become an important means for understanding cancer incidence and progression largely driven by the availability of high-throughput sequencing technologies for genomes, proteomes, and transcriptomes. However, how tumor cells from the site of origin of the cancer begin to grow in other sites of the body is very p.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038987
- DOI:
- 10.4238/gmr.15038987
MicroRNA-338-3p inhibits glucocorticoid-induced osteoclast formation through RANKL targeting
Authors: X.H. Zhang, G.L. Geng, B. Su, C.P. Liang, F. Wang and J.C. Bao
The differentiation deficiencies of osteoclast precursors (pre-OCs) may contribute to osteoporosis. Research on osteoporosis has recently focused on microRNAs (miRNAs) that play crucial roles in pre-OC differentiation. In the current study, we aimed to analyze the expression and function of the glucocorticoid (GC)-associated miRNA-338-3p (miR-338-3p) in o.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15037674
- DOI:
- 10.4238/gmr.15037674
Analysis of the prevalence of polymorphisms in the glutathione S transferase gene (GST) in cataract patients from Goi�¢nia
Authors: I.R. Costa, R.S. Mascarenhas, J.F. Corr�ªa, F.R.B. Oliveira, S.G. Fernandes, F.O. Souza, G.M. dos Reis, A. de P.R. J�ºnior, D.A. Rodrigues, J.V.M. Martins, K.S.F. e Silva and K.K.V.O. Moura
The aim of this study was to determine the prevalence of polymorphisms in the glutathione S-transferase genes GSTM1 and GSTT1 in patients with lens opacity (cataract). Peripheral blood samples were obtained from male and female patients (N = 23) with cataract. The GSTM1 and GSTT1 polymorphic regions were amplified by polymerase chain reaction, and the amp.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038050
- DOI:
- 10.4238/gmr.15038050
Utilization of different methodologies for the characterization of Hb Hasharon heterozygotes
Authors: A.R. Chinelato-Fernandes, C.F. Mendiburu and C.R. Bonini-Domingos
Hb Hasharon has an electrophoretic mobility similar to that of Hb S in cellulose acetate and a mobility between Hb S and C at acid pH. In high-performance liquid chromatography, Hb Hasharon shows a distinct chromatographic profile and retention time. The origin of this variant is a mutation in codon 47 (GAC → CAC) of the .. Read More»
- Genet. Mol. Res. 5(1):
Simplified preparation of a DNA ladder using PCR
Authors: T.-Y. Wang, L. Wang and F. Wang
Serving as a DNA molecular weight standard, the DNA ladder has been widely used in molecular biology applications. We developed a simple method for the preparation of a DNA marker, which involves designing primers to amplify 100- to 1000-bp DNA fragments using lambda DNA as a template for polymerase chain reaction, followed by.. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr1177
- DOI:
- 10.4238/vol10-3gmr1177
In silico characterization of 1,2-diacylglycerol cholinephosphotransferase and lysophosphatidylcholine acyltransferase genes in Glycine max L. Merrill
Authors: C.S. Sousa, B.A. Barros, D. Barh, P. Ghosh, V. Azevedo, E.G. Barros and M.A. Moreira
The enzymes 1,2-diacylglycerol cholinephosphotransferase (CPT) and lysophosphatidylcholine acyltransferase (LPCAT) are important in lipid metabolism in soybean seeds. Thus, understanding the genes that encode these enzymes may enable their modification and aid the improvement of soybean oil quality. In soybean, the gene.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038974
- DOI:
- 10.4238/gmr.15038974
Improved protocol for extracting genomic DNA from frozen formalin-fixed tissue resulting in high-quality whole mtDNA
Authors: H.W. Wang, Y. Xu, H.F. Zhang, Y.J. Zeng, L. Ren, Y.L. Miao, H.Y. Luo and K.H. Wang
Formalin fixation and paraffin embedding is widely used for convenient and long-term storage of tumor tissue and precious sources to perform genetic studies. However, DNA fragmentation is one of the major flaws of genomic DNA isolation from formalin fixation tissues, which limits its further usage. Here, we present an improved method for isolating high-qu.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15037972
- DOI:
- 10.4238/gmr.15037972
Activation of Src tyrosine kinase in esophageal carcinoma cells in different regulatory environments and corresponding occurrence mechanism
Authors: Z.S. Yu, X.H. Song, H.M. Gan, D.Z. Geng and J. Yang
This study aims at observing the expression of activated Src tyrosine kinase in esophageal squamous cell carcinoma (ESCC), and exploring the relationship of Src tyrosine kinase with the occurrence and progression of ESCC. Immunohistochemistry, immunofluorescence, and immunoblotting are employed to investigate the expression of Src tyrosine kinase in the E.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038026
- DOI:
- 10.4238/gmr.15038026
HLA-G genetic variants and hepatocellular carcinoma: a meta-analysis
Authors: A.V.C. Coelho, R.R. Moura, S. Crovella and F. Celsi
Human leukocyte antigen (HLA)-G is a key tolerogenic molecule mainly expressed in the placenta and is crucial for implantation of the embryo and immunological tolerance of the fetus during pregnancy. However, under pathological conditions, such as cancer or viral infections, HLA-G can be expressed in other tissues. The gene coding for HLA-G (HLA-G, chromo.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038263
- DOI:
- 10.4238/gmr.15038263