Abstract

In silico characterization of 1,2-diacylglycerol cholinephosphotransferase and lysophosphatidylcholine acyltransferase genes in Glycine max L. Merrill

Author(s): C.S. Sousa, B.A. Barros, D. Barh, P. Ghosh, V. Azevedo, E.G. Barros and M.A. Moreira

The enzymes 1,2-diacylglycerol cholinephosphotransferase (CPT) and lysophosphatidylcholine acyltransferase (LPCAT) are important in lipid metabolism in soybean seeds. Thus, understanding the genes that encode these enzymes may enable their modification
and aid the improvement of soybean oil quality. In soybean, the genesencoding these enzymes have not been completely described; therefore,
this study aimed to identify, characterize, and analyze the in silicoexpression of these genes in soybean. We identified two gene models
encoding CPT and two gene models encoding LPCAT, one of whichpresented an alternative transcript. The sequences were positioned on
the physical map of soybean and the promoter regions were analyzed.Cis-elements responsible for seed-specific expression and responses tobiotic and abiotic stresses were identified. Virtual expression analysisof the gene models for CPT and LPCAT indicated that these genes are
expressed under different stress conditions, in somatic embryos duringdifferentiation, in immature seeds, root tissues, and calli. Putative aminoacid sequences revealed the presence of transmembrane domains,and analysis of the cellular localization of these enzymes revealed theyare located in the endoplasmic reticulum.

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