Medical Genetics
Identification of key biomarkers involved in osteosarcoma using altered modules
Authors: Z.Z. Liu, S.T. Cui, B. Tang, Z.Z. Wang and Z.X. Luan
The aim of this study was to screen for key biomarkers of osteosarcoma (OS) by tracking altered modules. Protein-protein interaction (PPI) networks of OS and normal groups were constructed and re-weighted using the Pearson correlation coefficient (PCC), respectively. The condition-specific modules were explored from OS and normal PPI networks using a cliq.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038277
- DOI:
- 10.4238/gmr.15038277
Development and significance of SCAR marker QG12-5 for Canarium album (Lour.) Raeusch by molecular cloning from improved RAPD amplification
Authors: J.L. Cheng, Z.C. Yin Z.Q. Mei, C.L. Wei, H.C. Chen, X.S. Wu and J.J. Fu
Sequence-characterized amplified region (SCAR) is a valuable molecular marker for the genetic identification of any species.This marker is mainly derived from molecular cloning of random amplified polymorphic DNA (RAPD). We have previously reported the use of an improved RAPD technique for the genetic characterization of different samples of Canarium albu.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038347
- DOI:
- 10.4238/gmr.15038347
Construction of recombinant human IFN�±-2b BCG and its antitumor effects on bladder cancer cells in vitro
Authors: E. Sun, X. Nian, C. Liu, X. Fan and R. Han
We constructed recombinant Bacille Calmette-Guérin (rBCG) that secreted human interferon alpha 2b (hIFNa-2b), and investigated its antitumor effects on bladder cancer cells in vitro. The recombinant plasmid phIFN-a-2b was constructed using pMAO-4 and transformed into BCG. The supernatant was collected at various times and IFN-g, interleukin (IL)-12.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.15.7
- DOI:
- http://dx.doi.org/10.4238/2015.April.15.7
Effect of curcumin on p38MAPK expression in DSS-induced murine ulcerative colitis
Authors: C.-P. Li, J.-H. Li, S.-Y. He, O. Chen and L. Shi
The aim of this study was to determine the therapeutic effect of curcumin on dextran sulfate sodium-induced ulcerative colitis (UC) and to explore the related mechanism. Sixty mice were randomly divided into 6 groups. A group was the normal control group; B group was the model group; C group was the 1.5 mg/kg dexamethasone group based on the B group; and .. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.15.8
- DOI:
- http://dx.doi.org/10.4238/2015.April.15.8
Disease indicators for sepsis and analysis of sepsis treatment in children using the continuous blood purification technique
Authors: J.P. Liu, X.W. Wang and L.P. Qie
We analyzed disease severity, inflammation markers, and dynamic changes in cartilage glycoprotein 39 (YKL-40) and Creactive protein (CRP) levels in children with sepsis before and after treatment with continuous blood purification (CBP). Study participants were 30 children with severe sepsis who were cured from the disease (experimental group) in the Chil.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.25.21
- DOI:
- 10.4238/2015.May.25.21
Comparative study of clinical efficacy of laparoscopy-assisted radical gastrectomy versus open radical gastrectomy for advanced gastric cancer
Authors: L.M. Wu, X.J. Jiang, Q.F. Lin and C.X. Jian
The purpose of this study is to compare the efficacy of laparoscopy-assisted radical gastrectomy (LARG) versus that of open radical gastrectomy (ORG). Clinical data of 355 patients who underwent radical gastrectomy (160 in the LARG group and 195 in the ORG group) were analyzed retrospectively. Efficacy indices were compared and analyzed between the two gr.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.15.9
- DOI:
- http://dx.doi.org/10.4238/2015.April.15.9
Value of human brain natriuretic peptide in treatment of acute anterior myocardial infarction evaluated via three-dimensional speckle tracking imaging
Authors: Y. Zhang, F. Gao, X.-Y. Zhang, Y.-M. Hou, Y. Tang, S.-L. Yang,S.-H. Zhu, R.-Y. Luo and S.-F. Lin
Three-dimensional ultrasound speckle tracking imaging was used to evaluate the effects of recombinant human brain natriuretic peptide (rhBNP) in acute anterior and extensive anterior myocardial infarction. Ninety patients with acute anterior or extensive myocardial infarction were randomly divided into 3 groups: Group A [emergency percutaneous coronary in.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.29.2
- DOI:
- 10.4238/2015.May.29.2
Application of indirect linkage analysis and direct genotyping to hemophilia A carrier detection in Sichuan, China
Authors: P. Sun, L. Ma, G. Diao, C.Q. Li and F.Z. Lin
Hemophilia A (HA) is an inherited X-linked bleeding disorder caused by mutations in the factor VIII gene. Prenatal detection in female carriers from families with HA is important to reduce the number of HA patients. The purpose of this study was to detect carriers in families with HA from Sichuan, China, using linkage analysis and a direct genotyping meth.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.27.10
- DOI:
- 10.4238/2015.July.27.10
PUMA gene transfection can enhance the sensitivity of epirubicin-induced apoptosis of MCF-7 breast cancer cells
Authors: C.-G. Sun, J. Zhuang, W.-J. Teng, Z. Wang and S.-S. Du
We explored whether p53 upregulated modulator of apoptosis (PUMA) gene transfection could enhance the sensitivity of epirubicin-induced apoptosis of MCF-7 breast cancer cells. The liposome-mediated recombinant eukaryotic expression vector PUMA-pCDNA3 and empty vector plasmid were stably transfected into MCF-7 cells. Epirubicin (0.01-100 μM) was applied.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.29.6
- DOI:
- 10.4238/2015.May.29.6
Relevance of E-cadherin expression to EGFR-TKI molecular targeted therapy sensitivity/resistance and its clinical significance
Authors: R.C. Xing, J. Zheng, W.H. Zheng, Z.P. Qin, W. Liu and R.C. Yao
We examined the effect of E-cadherin expression on epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI) molecular targeted therapy sensitivity/resistance. We treated MCF-7, MDA-MB-231, T24, SiHa, H460, SK-HEP-1, MHCC97-H, and THP- 1 cells with the EGFR-TKIs PD153035 and gefitinib, and then tested the drug-resistance and sensitivity using .. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.29.10
- DOI:
- 10.4238/2015.May.29.10