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Cytogenomics   Research Article
Construction of suppressor of cytokine signaling 2 (SOCS2) adenoviral overexpression vector and its impact on growth hormone- induced lipolysis in swine primary adipocytes

Authors: H.L. Yang, J. Yan, M. Feng, X. Tan, G.Y. Yan, L. Gan and C. Sun

We investigated the effect of overexpression suppressor of cytokine signaling 2 (SOCS2) on lipolysis in swine primary adipocytes (pAd) induced by growth hormone (GH). We constructed pAd-SOCS2 adenoviral overexpression vectors to infect HEK293 cells for virus packaging and propagation. Cultured swine primary adipocytes were infected with virus particles; a.. Read More»

Genet. Mol. Res. 12(2): 2013.January.9.2

DOI: 10.4238/2013.January.9.2

Microbial Genetics   Research Article
Subtyping Brazilian Yersinia pestis strains by pulsed-field gel electrophoresis

Authors: M.P.S. Barros, V.M. Silveira-Filho, R.H.F.B. Lins, M.B.M. Oliveira, A.M.P. Almeida and T.C. Leal-Balbino

We subtyped Brazilian Yersinia pestis strains by pulsed-field gel electrophoresis (PFGE). This was done with 22 Brazilian Y. pestis strains: 17 from an outbreak and 5 from endemic routine surveillance. The strains were divided into 2 groups (I and II), 8 subgroups (A-H) and 19 PFGE profiles or pulsotypes. PFGE did not separate outbreak from non-outbreak s.. Read More»

Genet. Mol. Res. 12(2): 2013.January.4.23

DOI: 10.4238/2013.January.4.23

Cytogenomics   Research Article
A proposed model for the flowering signaling pathway of sugarcane under photoperiodic control

Authors: C.P. Coelho, A.P. Costa Netto, J. Colasanti and A. Chalfun-Junior

Molecular analysis of floral induction in Arabidopsis has identified several flowering time genes related to 4 response networks defined by the autonomous, gibberellin, photoperiod, and vernalization pathways. Although grass flowering processes include ancestral functions shared by both mono- and dicots, they have developed their own mechanisms to transmi.. Read More»

Genet. Mol. Res. 12(2): 2013.April.25.6

DOI: 10.4238/2013.April.25.6

Medical Genetics   Research Article
The insertion/deletion polymorphism in the ACE gene and chronic obstructive pulmonary disease

Authors: S. Simsek, S. Tekes, D. Oral, A. Turkyilmaz, B. Isik, M.R. Isik and H. Akkoc

An insertion/deletion (I/D) polymorphism was identified in intron 16 of the gene encoding the human angiotensin I-converting enzyme (ACE), a candidate gene for chronic obstructive pulmonary disease (COPD). We investigated the relationship between this polymorphism in the ACE gene and the risk of developing COPD. Sixty-six COPD in-patients and 40 non-smoki.. Read More»

Genet. Mol. Res. 12(2): 2013.April.25.10

DOI: 10.4238/2013.April.25.10

Cytogenomics   Research Article
Evaluation of genetic diversity in fig accessions by using microsatellite markers

Authors: A.D.B. do Val, C.S. Souza, E.A. Ferreira, S.M.L. Salgado, M. Pasqual and G.M.A. Can�§ado

Fig (Ficus carica L.) is a fruit of great importance worldwide. Its propagation is carried out with stem cuttings, a procedure that favors the occurrence of synonymy among specimens. Thus, molecular markers have become an important tool for studies of DNA fingerprinting, germplasm characterization, and genetic diversity evaluation in this plant species. T.. Read More»

Genet. Mol. Res. 12(2): 2013.April.25.9

DOI: 10.4238/2013.April.25.9

Cytogenomics   Research Article
CTLA-4 and CD86 genetic variants and haplotypes in patients with rheumatoid arthritis in southeastern China

Authors: C.-P. Liu, J.-A. Jiang, T. Wang, X.-M. Liu, L. Gao, R.-R. Zhu, Y. Shen, M. Wu, T. Xu and X.-G. Zhang

The cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) and costimulatory molecule (CD80/CD86) genes are important susceptibility genes associated with autoimmune diseases. CTLA-4 polymorphisms have been found to be associated with various autoimmune diseases. However, the association data are inconsistent for rheumatoid arthritis (RA). We investigated t.. Read More»

Genet. Mol. Res. 12(2): 2013.April.25.8

DOI: 10.4238/2013.April.25.8

Cytogenomics   Research Article
Genetic characterization of Fusarium graminearum and F. culmorum isolates from Turkey by using random-amplified polymorphic DNA

Authors: E. Y�¶r�¼k and G. Albayrak

Five Fusarium graminearum and 12 F. culmorum isolates, primarily pathogenic species of Fusarium head blight, were obtained from naturally infected wheat from various agro-ecological regions of Turkey. Genotyping of the isolates was carried out using random-amplified polymorphic DNA (RAPD). Sixty-five 10-mer oligonucleotide primers were used to amplify the.. Read More»

Genet. Mol. Res. 12(2): 2013.April.25.7

DOI: 10.4238/2013.April.25.7

Medical Genetics   Research Article
Evaluation of a histocompatibility antigen related to hepatitis B virus in patients with hepatocellular carcinoma in the western Brazilian Amazon

Authors: S.R.S. Chalub, R.M. da Silva, A.P.S. da Silva, P.F. de Aquino,A. Sadahiro and A.N. Kalil

Hepatocellular carcinoma is an infection of variable incidence that can be caused by hepatitis B virus (HBV), which is endemic in the Amazon region. The diagnosis of HBV can be performed through the use of serum markers such as the hepatitis B surface antigen. The chronic HBV can cause mutagenesis and carcinogenesis, being the susceptibility of infection .. Read More»

Genet. Mol. Res. 12(2): 2013.April.25.5

DOI: 10.4238/2013.April.25.5