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Plant Genetics   Research Article
Influence of GSTM1 and GSTT1 polymorphisms on the survival rate of patients with malignant glioma under perillyl alcohol-based therapy

Authors: M.M. Silva, C.O. Da Fonseca, R. Moura-Neto, J.F. Carvalho, T. Quirico-Santos and M.G. Carvalho

GSTM1 (glutathione S-transferase mu 1) and GSTT1 (glutathione S-transferase theta 1) are critical enzymes for detoxification of endogenous and environmental carcinogens. Constitutive GST gene polymorphisms may be associated with increased risk for cancer development. We made an explorative study of a Brazilian population with malignant glioma to determine.. Read More»

Genet. Mol. Res. 12(2): 2013.May.14.2

DOI: 10.4238/2013.May.14.2

Cytogenomics   Research Article
Descriptor selection for banana accessions based on univariate and multivariate analysis

Authors: L.P. Brand�£o, C.P.F. Souza, V.M. Pereira, S.O. Silva, J.A. Santos-Serejo, C.A.S. Ledo and E.P. Amorim

Our objective was to establish a minimum number of morphological descriptors for the characterization of banana germplasm and evaluate the efficiency of removal of redundant characters, based on univariate and multivariate statistical analyses. Phenotypic characterization was made of 77 accessions from Bahia, Brazil, using 92 descriptors. The selection of.. Read More»

Genet. Mol. Res. 12(2): 2013.May.14.1

DOI: 10.4238/2013.May.14.1

Plant Genetics   Research Article
Comparative in vitro and in planta analyses of extracellular enzymes secreted by the pathogenic fungus Sclerotinia sclerotiorum

Authors: M.B. Oliveira, S.C. Barbosa and S. Petrofeza

Dry bean (Phaseolus vulgaris L.) is an important economic crop in Brazil but its yield can be significantly reduced by white mold, a disease caused by Sclerotinia sclerotiorum (Lib.) de Bary, a necrotrophic, highly destructive, and non-host-specific fungus. This fungus secretes numerous cell wall-degrading enzymes such as polygalacturonases, exo-β-1,.. Read More»

Genet. Mol. Res. 12(2): 2013.June.6.3

DOI: 10.4238/2013.June.6.3

Plant Genetics   Research Article
Transferability of retrotransposon primers derived from Persimmon (Diospyros kaki Thunb.) across other plant species

Authors: X.Y. Du, Q.N. Hu, Q.L. Zhang, Y.B. Wang and Z.R. Luo

Retrotransposon-based molecular markers are powerful molecular tools. However, these markers are not readily available due to the difficulty in obtaining species-specific retrotransposon primers. Although recent techniques enabling the rapid isolation of retrotransposon sequences have facilitated primer development, this process nonetheless remains time-c.. Read More»

Genet. Mol. Res. 12(2): 2013.June.6.2

DOI: 10.4238/2013.June.6.2

Plant Genetics   Research Article
Development of universal primers for isolating fragments of the LEAFY gene

Authors: L.X. Yu and B. Yan

LEAFY orthologs are found throughout land plants. Degenerate primers were designed for amplifying exon 3 region fragments of the LEAFY gene. Sixteen taxa from different plants were sampled, all of which were successfully isolated, which indicated that this set of primers is universally applicable for all land plants. With the use of this method, there may.. Read More»

Genet. Mol. Res. 12(2): 2013.June.6.1

DOI: 10.4238/2013.June.6.1

Animal Genetics   Research Article
Effect of dietary Bacillus subtilis on proportion of Bacteroidetes and Firmicutes in swine intestine and lipid metabolism

Authors: C. Cui, C.J. Shen, G. Jia and K.N. Wang

The ratio of Bacteroidetes and Firmicutes bacterial groups in the gut can affect the ability to absorb nutrients. We investigated the effect of probiotic Bacillus subtilis supplementation of diets on growth performance, fat deposition, blood lipids, copy numbers, and percentage of Bacteroidetes and Firmicutes in cecal contents, as well as mRNA expression .. Read More»

Genet. Mol. Res. 12(2): 2013.May.23.1

DOI: 10.4238/2013.May.23.1

Animal Genetics   Research Article
Development of new polymorphic microsatellite markers in topmouth culter (Culter alburnus) and determination of their applicability in Culter mongolicus

Authors: P.-Z. Qi, C.-X. Xie, B.-Y. Guo, C.-W. Wu, S.-M. Lu and Y.-J. Duan

Fifteen new polymorphic microsatellite markers were developed for Culter alburnus. In 32 individuals representing a wild population of the Danjiangkou Reservoir, Hubei, China, the number of alleles at these loci varied between 2 and 10, with an average of 5.5. The average observed and expected heterozygosities were 0.664 and 0.681, respectively. The polym.. Read More»

Genet. Mol. Res. 12(2): 2013.May.21.7

DOI: 10.4238/2013.May.21.7

Bioinformatics   Research Article
Application of denaturing gradient gel electrophoresis for detection of bacterial and yeast communities along a salinity gradient in the estuary of the Cachoeira River in Brazil

Authors: C.S.P. Rodrigues, S.S. Souza, R.P. Rezende, A. Silva, J.L. Andrioli, H. Costa, R. Fontana and J.C.T. Dias

An estuary is a transition zone between freshwater and marine ecosystems, resulting in dilution of seawater. Estuaries are also considered environments of intense biological activity related to the processes of nutrient cycling. The aim of this study was to evaluate the microbial community composition along a salinity gradient in the estuary of the Cachoe.. Read More»

Genet. Mol. Res. 12(2): 2013.May.21.6

DOI: 10.4238/2013.May.21.6