Microbial Genetics
Overexpression of HSP27 in cultured human aortic smooth muscular cells reduces apoptosis induced by low-frequency and low-energy ultrasound by inhibition of an intrinsic pathway
Authors: B. Zhang, H.S. Zhou, Q. Cheng, L. Lei and B. Hu
We investigated in vitro the effect of low-frequency and low-energy ultrasound (LFLEU) on apoptosis of an overexpressed HSP27 human aortic smooth muscle cell (HASMC) line. A frequency of 42.6 kHz was used in all experiments. HASMC were exposed to ultrasound and cell viability was evaluated by MTT reduction. Overexpressed HSP27-HASMC was constructed on a p.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.11.10
- DOI:
- 10.4238/2013.December.11.10
Morphological and genetic characteristics of hybrid combinations of Dactylis glomerata
Authors: Y.F. Zhao, X.Q. Zhang, X. Ma, W.G. Xie and L.K. Huang
Six F1 populations derived from crosses among 4 orchardgrass (Dactylis glomerata L.) cultivars were studied by morphological and simple sequence repeat molecular markers to test for hybrid vigor and a correlation between genetic distance and heterosis. Heterosis was observed for days to length of culm, leaf traits, tiller numb.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.January.28.1
- DOI:
- 10.4238/2014.January.28.1
Separation, purification, and identification of flagellin, and preparation of its antisera
Authors: M.D. Hu1, G.S. Wang1, J. Xu1, W. Yao1, B.F. He1, Y. Yang1, M. Mao2, Q. Wang3 and J.C. Xu1
The aim of this study was to separate, purify, and identify Salmonella paratyphi A flagellin, and to prepare its antisera. Primary flagellin was isolated from S. paratyphi A using the acid lysis method. The flagellin was purified with weak anion exchange chromatography and the protein was identified with sodium dodecyl sulfate-polyacrylamide gel electroph.. Read More»
- Genet. Mol. Res. 13(4):
Gene expression and molecular modeling of the HSP104 chaperone of Trypanosoma cruzi
Authors: R.A. Campos, M.L. da Silva, G.V. da Costa, P.M. Bisch, J.M. Peralta, R. Silva, E. Rondinelli and T.P. ��rm�©nyi
Heat shock protein (HSP) 104 is a highly conserved molecular chaperone that catalyzes protein unfolding, disaggregation and degradation under stress conditions. We characterized HSP104 gene structure and expression in Trypanosoma cruzi, a protozoan parasite that causes Chagas’ disease. The T. cruzi HSP104 is an 869 amino-acid protein encoded by a si.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.August.6.15
- DOI:
- 10.4238/2012.August.6.15
Thirty-four Musa (Musaceae) expressed sequence tag-derived microsatellite markers transferred to Musella lasiocarpa
Authors: W.J. Li, H. Ma, Z.H. Li, Y.M. Wan, X.X. Liu and C.L. Zhou
We assembled 31,308 publicly available Musa EST sequences into 21,129 unigenes; 4944 of them contained 5416 SSR motifs. In all, 238 unigenes flanking SSRs were randomly selected for primer design and then tested for amplification in Musella lasiocarpa. Seventy-eight primer pairs were found to be transferable to this species, and 49 displayed polymorphism... Read More»
- Genet. Mol. Res. 11(3):
- 2012.August.6.13
- DOI:
- 10.4238/2012.August.6.13
A new strategy employed for identification of sweet orange cultivars with RAPD markers
Authors: X. Sun, Q. Mu, D. Jiang , C. Wang, X.C. Wang and J.G. Fang
We optimized RAPD techniques by increasing the length of RAPD primers and performing a strict screening of PCR annealing temperature to distinguish 60 sweet orange cultivars from the Research Institute of Pomology at the Chinese Academy of Agricultural Sciences. A new approach called cultivar identification diagram (CID) was used to improve the efficiency.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.August.6.11
- DOI:
- 10.4238/2012.August.6.11
Molecular combing in the analysis of developmentally regulated amplified segments of Bradysia hygida
Authors: K.J.R. Passos, S.Y. Togoro, S. Carignon, S. Koundrioukoff, A.-M. Lachages, M. Debatisse and M.A. Fernandez
Molecular combing technology is an important new tool for the functional and physical mapping of genome segments. It is designed to identify amplifications, microdeletions, and rearrangements in a DNA sequence and to study the process of DNA replication. This technique has recently been used to identify and analyze the dynamics of replication in amplified.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.August.6.10
- DOI:
- 10.4238/2012.August.6.10
Assessment of genetic diversity of landraces of Dendrocalamus hamiltonii using AFLP markers and association with biochemical traits
Authors: S.D. Waikhom, S. Ghosh, N.C. Talukdar and S.S. Mandi
Fermented bamboo shoots are popular traditional food items of various ethnic groups of the northeastern India, especially in Manipur State. Dendrocalamus hamiltonii is an economically important bamboo species used to produce fermented bamboo shoots. We studied genetic variability of this bamboo species in Chandel and Imphal-East (commercial production dis.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.June.21.1
- DOI:
- 10.4238/2012.June.21.1
Identification of hybrids between triticale and Aegilops juvenalis (Thell.) Eig and determination of genetic similarity with ISSRs
Authors: A. Gradzielewska, D. Gruszecka, J. LeÃ?Â?niowska-Nowak and E. Paczos-GrzÃ?Â?da
Wide hybridization expands the gene pool of cultivated species and can produce genotypes with practical value, often resistant to diseases. In plants, ISSRs are often used for hybrid identification as well as for diversity evaluation. We estimated genetic similarity among advanced genetic stocks of triticale hybrids and identified Aegilops juvenalis intro.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.July.10.1
- DOI:
- 10.4238/2012.July.10.1
Effect of different carbon sources on proteases secreted by the fungal pathogen Sclerotinia sclerotiorum during Phaseolus vulgaris infection
Authors: E.A. Bueno, M.B. Oliveira, R.V. Andrade, M. Lobo Junior and S. Petrofeza
Sclerotinia sclerotiorum (Sclerotiniaceae) is a plant pathogenic fungus that causes white mold disease in vegetable crops, including the common bean (Phaseolus vulgaris). Proteases produced by fungi are normally an important part of the pathogenic process in the host. We examined the effect of different carbon sources - pectin, glucose, and cell wall of P.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.June.25.3
- DOI:
- 10.4238/2012.June.25.3