Microbial Genetics
Heterologous protein production and delivery systems for Lactococcus lactis
Authors: S�©bastien Nouaille, Luciana A. Ribeiro, Anderson Miyoshi, Daniela Pontes, Yves Le Loir, Sergio Costa Oliveira, Philippe Langella Vasco Azevedo
Lactic acid bacteria (LAB), widely used in the food industry, are present in the intestine of most animals, including humans. The potential use of these bacteria as live vehicles for the production and delivery of heterologous proteins of vaccinal, medical or technological interest has therefore been extensively investigated. .. Read More»
- Genet. Mol. Res. 2(1):
Operon mer: Bacterial resistance to mercury and potential for bioremediation of contaminated environments
Authors: Andr�©a M.A. Nascimento, Edmar Chartone-Souza
Mercury is present in the environment as a result of natural processes and from anthropogenic sources. The amount of mercury mobilized and released into the biosphere has increased since the beginning of the industrial age. Generally, mercury accumulates upwards through aquatic food chains, so that organisms at higher trophic .. Read More»
- Genet. Mol. Res. 2(1):
Escherichia coli as a model system to study DNA repair genes of eukaryotic organisms
Authors: Luiz Augusto-Pinto, Carlos Gustavo Regis da Silva, D�©bora de Oliveira Lopes, Alice Machado-Silva, Carlos Renato Machado
The bacteria Escherichia coli has been widely employed in studies of eukaryotic DNA repair genes. Several eukaryotic genes have been cloned by functional complementation of mutant lineages of E. coli. We examined the similarities and differences among bacterial and eukaryotic DNA repair systems. Based on these data, we examine.. Read More»
- Genet. Mol. Res. 2(1):
Staphylococcus aureus and food poisoning
Authors: Yves Le Loir, Florence Baron, Michel Gautier
Food-borne diseases are of major concern worldwide. To date, around 250 different food-borne diseases have been described, and bacteria are the causative agents of two thirds of food-borne disease outbreaks. Among the predominant bacteria involved in these diseases, Staphylococcus aureus is a leading cause of gastroenteritis r.. Read More»
- Genet. Mol. Res. 2(1):
Efflux as a mechanism of resistance to antimicrobials in Pseudomonas aeruginosa and related bacteria: unanswered questions
Authors: Herbert P. Schweizer
Pseudomonas aeruginosa is an opportunistic human pathogen exhibiting innate resistance to multiple antimicrobial agents. This intrinsic multidrug resistance is caused by synergy between a low-permeability outer membrane and expression of a number of broadly-specific multidrug efflux (Mex) systems, including MexAB-OprM and MexX.. Read More»
- Genet. Mol. Res. 2(1):
RAPD analysis of herbicide-resistant Brasilian rice lines produced via mutagenesis
Authors: S.S. Sandhu, C�¢ndido R. Bastos, Luiz Ernesto Azini, Augusto Tulmann Neto, Carlos Colombo
Over the last two decades, mutational techniques have become one of the most important tools available to progressive rice- breeding programs. In a mutation-breeding program initiated in 1999 at the Instituto Agronômico of Campinas, SP, Brazil, a rice line, IAC103, was selected for mutational studies with gamma radiation.. Read More»
- Genet. Mol. Res. 1(4):
Intraspecific variability of Bipolaris sorokiniana isolates determined by random-amplified polymorphic DNA (RAPD)
Authors: Aida T.S. Matsumura, Ariano M. Prestes, Sueli T. Van Der Sand, Andr�©ia M.R. de Oliveir
Isolates of Bipolaris sorokiniana were analyzed by random-amplified polymorphic DNA (RAPD) techniques to determine the amount of intraspecific genetic variability and to study host-pathogen interactions. Ten isolates originated from different regions of Brazil were examined. Plants of the wheat cultivars BR8, BH1146 (original .. Read More»
- Genet. Mol. Res. 1(4):
Micronucleus investigation of alcoholic patients with oral carcinomas
Authors: Andr�©a Ramirez, Pedro Henrique Saldanha
The micronucleus test (MN) is used as an indicator of genotoxic exposition, since it is associated with chromosome aberrations. An increased mutation rate in oral squamous cells, indicated by an increased MN frequency, is also related to the development of oral carcinomas. We evaluated the frequencies of MN and other metanucle.. Read More»
- Genet. Mol. Res. 1(3):
Rapid identification of single nucleotide polymorphisms by fluorescence-based capillary electrophoresis
Authors: Montse Bernat, Esther Titos and Joan Cl� ria
We describe the application of two different fluorescence- based techniques (ddNTP primer extension and single-strand conformation polymorphism (SSCP)) to the detection of single nucleotide polymorphisms (SNPs) by capillary electrophoresis. The ddNTP primer extension technique is based on the extension, in the presence of fluo.. Read More»
- Genet. Mol. Res. 1(1):
Detection of stabilizing selection in favor of the Santa Cruz homokaryotype in Drosophila pseudoobscura populations from the high plateau of the Colombian Andes
Authors: Diana Alvarez, Manuel Ruiz-Garcia, Jimena Guerrero and Juan P. Jaramillo
Chromosome 3 rearrangements were studied in five Drosophila pseudoobscura populations from the high plateau of the Colombian Andes. As in previous studies, the Santa Cruz and Tree Line rearrangements were predominant in these populations, but for the first time other rearrangements such as the Olympic, Cuernavaca and a rearran.. Read More»
- Genet. Mol. Res. 1(1):