Microbial Genetics
Screening and identification of microsatellite markers associated with cold tolerance in Nile tilapia Oreochromis niloticus
Authors: H.P. Zhu, Z.G. Liu, M.X. Lu, F.Y. Gao, X.L. Ke and Z.H. Huang
Tilapia is an important fish cultured in tropical and subtropical areas. Cold sensitivity limits the expansion of tilapia culture into colder regions of the world, and mass mortalities of cultured tilapia have been reported due to severe cold currents in winter. Since the late 1990s, several strains of Nile tilapia have been domesticated to improve the ab.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.28.16
- DOI:
- 10.4238/2015.August.28.16
Functional characterization and analysis of the Arabidopsis UGT71C5 promoter region
Authors: Z. Liu, Q.H. Luo, J.M. Wang, X.F. Li and Y. Yang
In the present study, we isolated an Arabidopsis promoter, UGT71C5, and analyzed its role in the regulation of the light response mechanism. We constructed a fusion vector pBI121-pU-GUS by integrating the UGT71C5 promoter upstream of the GUS reporter gene in pBI121, and then transferred this vector into Arabidopsis plants. The GUS activity of the transgen.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.29.27
- DOI:
- 10.4238/2015.December.29.27
Streptococcus massiliensis in the human mouth: a phylogenetic approach for the inference of bacterial habitats
Authors: F. P�³ntigo, C. Silva, M. Moraga and S.V. Flores
Streptococcus is a diverse bacterial lineage. Species of this genus occupy a myriad of environments inside humans and other animals. Despite the elucidation of several of these habitats, many remain to be identified. Here, we explore a methodological approach to reveal unknown bacterial environments. Specifically, we inferred the phylogeny of the Mitis gr.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.29.28
- DOI:
- 10.4238/2015.December.29.28
Analysis of drug-resistant gene detection of blaOXA-like genes from Acinetobacter baumannii
Authors: D.K. Yang, H.J. Liang, H.L. Gao, X.W. Wang and Y. Wang
Our study determines the resistance gene profile of a set of Acinetobacter baumannii hospital isolates. A. baumannii is responsible for nosocomial outbreaks and sporadic infections. We extracted and PCR amplified bacterial DNA isolated from patients with ages below 60 years (23.36%) and above 60 years (76.64%). Most of the patients were admitted in the IC.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.29.7
- DOI:
- 10.4238/2015.December.29.7
Characterization and functional analysis of a chitin synthase gene (HcCS1) identified from the freshwater pearlmussel Hyriopsis cumingii
Authors: H.F. Zheng, Z.Y. Bai, J.Y. Lin, G.L. Wang, J.L. Li
The triangle sail mussel, Hyriopsis cumingii, is the most important freshwater pearl mussel in China. However, the mechanisms underlying its chitin-mediated shell and nacre formation remain largely unknown. Here, we characterized a chitin synthase (CS) gene (HcCS1) in H. cumingii, and analyzed its possible physiological function. The complete ORF sequence.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.29.36
- DOI:
- 10.4238/2015.December.29.36
Salmonella enterica Typhimurium fljBA operon stability: implications regarding the origin of Salmonella enterica I 4,[5],12:i:-
Authors: M.P.O. Tomiyama, C.H. Werle, G.P. Milanez, D.B. N�³brega, J.P. Pereira, A.P. Calarga, F. Flores and M. Brocchi
Salmonella enterica subsp enterica serovar 4,5,12:i:- has been responsible for many recent Salmonella outbreaks worldwide. Several studies indicate that this serovar originated from S. enterica subsp enterica serovar Typhimurium, by the loss of the flagellar phase II gene (fljB) and adjacent sequences. However, at least two different clones of S. enterica.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.29.13
- DOI:
- 10.4238/2015.December.29.13
Linker length affects expression and bioactivity of the onconase fusion protein in Pichia pastoris
Authors: G.G. Yang, X.Y. Xu, Y. Ding, Q.Q. Cui, Z. Wang, Q.Y. Zhang, S.H. Shi, Z.Y. Lv, X.Y. Wang, J.H. Zhang, R.G. Zhang and C.S. Xu
The aim of this study was to analyze the effect of linker length on the expression and biological activity of recombinant protein onconase (ONC) in fusion with human serum albumin (HSA) in Pichia pastoris. Four flexible linkers with different lengths namely Linker L0, L1: (GGGGS)1, L2: (GGGGS)2, and L3:(GGGGS)3 were inserted into the fusion gene and refer.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.29.46
- DOI:
- 10.4238/2015.December.29.46
Direct preparation protocol to obtain mitotic chromosomes from canine mammary tumors
Authors: C.S.D. Morais, P.R.A.M. Affonso, J.A. Bitencourt and A.A. Wenceslau
Currently, mammary neoplasms in female canines are a serious problem in veterinary clinics. In addition, the canine species is an excellent disease model for human oncology because of the biological and genetic similarities between the species. Cytogenetics has allowed further study of the characterization of neoplasms in canines. We hypothesized that the.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.29.49
- DOI:
- 10.4238/2015.December.29.49
Glucagon-like peptide-1 receptor agonists inhibit hepatic stellate cell activation by blocking the p38 MAPK signaling pathway
Authors: L.K. Wu, Y.C. Liu, L.L. Shi and K.D. Lu
We investigated the effects of glucagon-like peptide-1 receptor (GLP-1R) agonists on p38 mitogen-activated protein kinase (MAPK) signaling during inhibition of hepatic stellate cell (HSC) activity. Human HSCs were cultured and morphologically identified. HSC samples were collected and randomly divided into three groups (N = 20 samples per group): a contro.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.29.17
- DOI:
- 10.4238/2015.December.29.17
Easy detection of green fluorescent proteinmulticopy transformants in Penicillium griseoroseum
Authors: Francis J.F. Lopes, Elza F. de Ara�ºjo and Marisa V. de Queiroz
Penicillium griseoroseum, a deuteromycete fungus producer of pectinolytic enzymes, was transformed with a gene encoding for green fluorescent protein (GFP). The selection of transformants was based on the homologous nitrate reductase gene (niaD). Protoplasts of a P. griseoroseum Nia mutant (PG63) were co-transformed with the p.. Read More»
- Genet. Mol. Res. 3(4):