Microbial Genetics
Non-Darwinian and Darwinian prokaryotic and eukaryotic evolution - an enigma in cell biology conservation
Authors: Hugo Hoenigsberg
Our theory is embarrassingly simple. What made today’s prokaryotes and modern cyanobacteria so robust is the fact that in their origin, back in the Archean (3 billion years ago), selection did not play a central role in evolution, it had only a transitory role. Asexual reproduction, mutation, drift and sampling variance .. Read More»
- Genet. Mol. Res. 2(3):
Cassava, Manihot esculenta Crantz genetic resources: VI. Anatomy of a diversity center
Authors: N.M.A. Nassar
Central Brasil is one of the four centers of diversity of Manihot species. Of 26 wild Manihot species reported to occur in this region, 20 were collected from two limited areas, each less than 100 km in diameter. These two areas are Goiás Velho and Corumbá de Goiás. Considering the Harlan concept of geogra.. Read More»
- Genet. Mol. Res. 2(2):
Diallel analysis of production traits among domestic, exotic and mutant germplasms of Lycopersicon
Authors: Guillermo Pratta, Liliana A. Picardi, Roxana Zorzoli
The effects of wild germplasm on tomato fruit shelf life have not yet been completely evaluated. Three different genotypes of Lycopersicon esculentum (a cultivated variety, a homozygote for nor and a homozygote for rin), LA1385 of L. esculentum var. cerasiforme, LA722 of L. pimpinellifolium, and 10 diallel hybrids were assayed.. Read More»
- Genet. Mol. Res. 2(2):
Comparative study of the cuticular hydrocarbon composition of Melipona bicolor Lepeletier, 1836 (Hymenoptera, Meliponini) workers and queens
Authors: F?¡bio C. Abdalla, Graeme R. Jones, Erik D. Morgan, Carminda da Cruz-Landim
In social insects, cuticular hydrocarbons are involved in species, kin, caste and nestmate recognition. Gas chromatography and mass spectrometry were used to compare the cuticular hydrocarbon composition of workers, males and queens of Melipona bicolor. The cuticular hydrocarbon composition of this species was found to consist.. Read More»
- Genet. Mol. Res. 2(2):
Mining microorganism EST databases in the quest for new proteins
Authors: Alessandra Concei?§?£o Faria-Campos, Gustavo Coutinho Cerqueira, Charles Anacleto, Jos?© Miguel Ortega, Cl?¡udia M?¡rcia Benedetto de Carvalho
Microorganisms with large genomes are commonly the subjects of single-round partial sequencing of cDNA, generating expressed sequence tags (ESTs). Usually there is a great distance between gene discovery by EST projects and submission of amino acid sequences to public databases. We analyzed the relationship between available E.. Read More»
- Genet. Mol. Res. 2(1):
Stage-specific gene expression during Trypanosoma cruzi metacyclogenesis
Authors: Andr?©a Rodrigues ?vila, Bruno Dallagiovanna, Sueli Fumie Yamada-Ogatta, Viviane Monteiro-G?³es, St?ªnio Perdig?£o Fragoso Marco Aur?©lio Krieger, Samuel Goldenberg
The process of Trypanosoma cruzi metacyclogenesis involves the transformation of noninfective epimastigotes into metacyclic trypomastigotes, which are the pathogenic form. The analysis of stage-specific genes during T. cruzi metacyclogenesis may provide insight into the mechanisms involved in the regulation of gene expression .. Read More»
- Genet. Mol. Res. 2(1):
Control of gene expression and genetic manipulation in the Trypanosomatidae
Authors: Santuza M.R. Teixeira, Wanderson D. da Rocha
Mechanisms controlling gene expression in trypanosomatids depend on several layers of regulation, with most regulatory pathways acting at a post-transcriptional level. Consequently, these parasites can follow the rapid changes associated with transitions between the insect vector and the mammalian host, with instant reprogramm.. Read More»
- Genet. Mol. Res. 2(1):
Sex in fungi: lessons of gene regulation
Authors: Cristina A.J. Souza, Carla C. Silva, Adlane V.-B. Ferreira
Fungi have been very useful for gene regulation studies. Mating implicates in a series of events influenced by many types of environmental input that are interpreted into regulatory pathways, including signal transduction. Although various aspects of mating and signal transduction in the yeast Saccharomyces cerevisiae have lon.. Read More»
- Genet. Mol. Res. 2(1):
Evaluation of alternative reporter genes for the yeast two-hybrid system
Authors: Alessandra L. Starling, Kenneth J. Gollob, Elisabete J. Vicente, Gisele M. Andrade-N?³brega, M?´nica B. Rodriguez, J. Miguel Ortega
The yeast two-hybrid system is a powerful tool for screening protein-protein interactions and has also been used for large-scale studies. We evaluated two protein-coding sequences as reporter genes for the yeast two-hybrid system, to determine if it was suitable as an alternative screening strategy. Aspergillus awamori glucoam.. Read More»
- Genet. Mol. Res. 2(1):
Challenges when transferring technology from Lactococcus laboratory strains to industrial strains
Authors: Eric Johansen
Many genetically modified Lactococcus strains have been constructed in research laboratories around the world. Most of these have originated from laboratory strains and therefore there are several barriers to using them in an industrial setting. Laboratory strains are often plasmid-free and consequently Lac- and Prt-, renderin.. Read More»
- Genet. Mol. Res. 2(1):