Microbial Genetics
Molecular phylogenetic relationships among members of the family Phytolaccaceae sensu lato inferred from internal transcribed spacer sequences of nuclear ribosomal DNA
Authors: J. Lee, S.Y. Kim, S.H. Park and M.A. Ali
The phylogeny of a phylogenetically poorly known family, Phytolaccaceae sensu lato (s.l.), was constructed for resolving conflicts concerning taxonomic delimitations. Cladistic analyses were made based on 44 sequences of the internal transcribed spacer of nuclear ribosomal DNA from 11 families (Aizoaceae, Basellaceae, Didiereaceae, Molluginaceae, Nyctagin.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.February.28.15
- DOI:
- 10.4238/2013.February.28.15
Maternal MTHFR C677T polymorphism and congenital heart defect risk in the Chinese Han population: a meta-analysis
Authors: K.H. Chen, L.L. Chen, W.G. Li, Y. Fang and G.Y. Huang
Numerous studies have evaluated the association between the maternal C677T polymorphism in the methylenetetrahydrofolate reductase (MTHFR) gene and congenital heart defect (CHD) risk in the Chinese Han population. However, the specific association is still controversial. Six separate studies with 1089 subjects in the Chinese Han population on the relation.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.4.8
- DOI:
- 10.4238/2013.December.4.8
Mitochondrial and microsatellite DNA analyses showed comparative genetic diversity between parent and offspring populations of Korean black rockfish in a hatchery facility
Authors: H.S. An, J.W. Lee, J.Y. Park, J.I. Myeong and C.M. An
The black rockfish, Sebastes inermis (Sebastidae), is an important commercial fishery resource in Korea. As a preliminary investigation into the effect of artificial reproduction in a hatchery facility, the genetic divergence between parent and offspring populations of black rockfish was accessed using 10 polymorphic nuclear microsatellite DNA loci and a .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.9.2
- DOI:
- 10.4238/2013.December.9.2
Validation of quantitative fluorescent-PCR for rapid prenatal diagnosis of common aneuploidies in the Chinese population
Authors: A.-Q. Xu, M. Xia, J.-T. Liu, F.-X. Yao, W.-M. Zhang, N. Hao, J. Zhou and X.-M. Bian
Quantitative fluorescent polymerase chain reaction (QF-PCR) is an accurate and reliable method for rapid detection of aneuploidy; however, it is not routinely used in China. We aimed to validate QF-PCR as a means for prenatal common aneuploidy screening and to analyze the heterozygosities of short tandem repeat (STR) markers in the Chinese population. The.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.9.1
- DOI:
- 10.4238/2013.December.9.1
Analysis of the genetic variability and structure of Ochlerotatus taeniorhynchus (Diptera: Culicidae) populations from the Colombian Atlantic coast on the basis of random amplified polymorphic DNA markers
Authors: F.J. Bello, N.A. Segura and M. Ruiz-Garcia
Ochlerotatus taeniorhynchus (Diptera: Culicidae) is a mosquito, which is an efficient vector of the virus causing epidemic-epizootic Venezuelan equine encephalitis in Colombia. This study used 9 random amplified polymorphic DNA (RAPD) markers to analyze the mosquito’s genetic variability and genetic structure of 122 spec.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.30.6
- DOI:
- http://dx.doi.org/10.4238/2014.May.30.6
Diversity of endophytic bacteria in Caragana microphylla grown in the desert grassland of the Ningxia Hui Autonomous Region of China
Authors: J.X. Dai, X.M. Liu and Y.J. Wang1
The diversity of endophytic bacteria in the sand-fixation plant Caragana microphylla was investigated by amplified rDNA restriction analysis and by sequence and phylogenetic comparisons of the 16S rRNA genes. A total of 24, 19, and 17 operational taxonomic units were identified from 16S rDNA libraries of the plant roots, stems.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.3.7
- DOI:
- 10.4238/2014.April.3.7
Sos recruitment system for the analysis of the interaction between sulfatase-modifying factor 2 subtypes and interleukin-13
Authors: W.Y. Zhang1*, H.Y. Liang1,2*, C. Yang1, L. Xue1 and X.F. Jiang1,2
(IL)-13 is a central mediator in allergic asthma. Our previous results have indicated that sulfatase-modifying factor 2 (SUMF2) interacts with IL-13 and inhibits its secretion. In this study, we investigated the interactions between SUMF2 subtypes and 2 types of IL-13. Wild type IL-13 (wh-IL-13) and its mutated counterpart (mh-IL-13) were analyzed and clo.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.18.15
- DOI:
- 10.4238/2013.November.18.15
Agrobacterium tumefaciens-mediated transformation of Lasiodiplodia theobromae, the causal agent of gummosis in cashew nut plants
Authors: C.R. Muniz, G.F. da Silva, M.T. Souza Jr., F.C.O. Freire, G.H.J. Kema and M.I.F. Guedes
Lasiodiplodia theobromae is a major pathogen of many different crop cultures, including cashew nut plants. This paper describes an efficient Agrobacterium tumefaciens-mediated transformation (ATMT) system for the successful delivery of T-DNA, transferring the genes of green fluorescent protein (gfp) and hygromycin B phosphotra.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.February.21.8
- DOI:
- 10.4238/2014.February.21.8
New support vector machine-based method for microRNA target prediction
Authors: L. Li, Q. Gao, X. Mao and Y. Cao
MicroRNA (miRNA) plays important roles in cell differentiation, proliferation, growth, mobility, and apoptosis. An accurate list of precise target genes is necessary in order to fully understand the importance of miRNAs in animal development and disease. Several computational methods have been proposed for miRNA target-gene id.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.June.9.3
- DOI:
- http://dx.doi.org/10.4238/2014.June.9.3
Expression of 1alpha-HYD and 24-HYD in bovine kidney mediated by vitamin D3 supplementation
Authors: L.R. Rezende, E.F. Delgado, A.R.L. J�ºnior, G. Gasparin, E.C. Jorge, G.B. Mour�£o and L.L. Coutinho
order to better understand vitamin D3 in cattle metabolism, we quantified 1alpha-HYD and 24-HYD gene expression. In the kidneys of 35 male Nellore cattle, these were divided into a control group and two treatment groups (2 x 106 international units of vitamin D3 administered for 2 or 8 consecutive days pre-slaughter). Vitamin D3 supplementation resulted i.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.11.12
- DOI:
- 10.4238/2013.December.11.12