Microbial Genetics
Analysis of cell cycle shortening and developmental interruption in Dictyostelium discoideum allC RNAi mutants
Authors: D.M. Xue, L.S. Hou, S.R. Zhang and N.X. Chen
Dictyostelium discoideum allC RNAi mutant cells are motile and aggregate together, but do not undergo further morphological development. The relatively quick growth rate of allC RNAi mutants compared to wild-type D. discoideum results in a shortened mutant cell cycle. However, at present, little is known about the mechanism un.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.23.6
- DOI:
- http://dx.doi.org/10.4238/2014.May.23.6
Relationship between genetic parameters in maize (Zea mays) with seedling growth parameters under 40-100% soil moisture conditions
Authors: R.W. Muhammad and A. Qayyum
We estimated the association of genetic parameters with production characters in 64 maize (Zea mays) genotypes in a green house in soil with 40-100% moisture levels (percent of soil moisture capacity). To identify the major parameters that account for variation among the genotypes, we used single linkage cluster analysis and principle component analysis. .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.18.15
- DOI:
- 10.4238/2013.October.18.15
Protein-protein interaction network and significant gene analysis of osteoporosis
Authors: X.M. Wu, X. Ma, C. Tang, K.N. Xie, J. Liu, W. Guo, Y.L. Yan, G.H. Shen and E.P. Luo
This study used DNA microarray data to identify differentially expressed genes of osteoporosis and provide useful information for treatments of the disease. We downloaded gene expression data of Osteoporosis GSE35956 from the Gene Expression Omnibus database, which included five normal and five osteoporosis samples. We then identified the differentially e.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.18.12
- DOI:
- 10.4238/2013.October.18.12
Comparative sodium dodecylsulfate-polyacrylamide gel electrophoresis and restricted fragment length polymorphism among fenugreek accessions
Authors: E.A. Haliem and A.A. Al-Huqail
Protein and DNA polymorphismswere surveyed among seven accessions of wild fenugreek (Trigonellafoenum-graecum L.) to estimate their genetic diversity and relationships. Samples were obtained from diverse ecogeographical areas in Saudi Arabia and Yemen. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of seed storage protein sh.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.4.16
- DOI:
- 10.4238/2013.December.4.16
Molecular cloning and expression analysis of two sex-lethal homolog genes during development in the oriental river prawn, Macrobrachium nipponense
Authors: Y.P. Zhang, H. Qiao, W.Y. Zhang, S.M. Sun, S.F. Jiang, Y.S. Gong, Y.W. Xiong, S.B. Jin and H.T. Fu
In this study, two Sxl gene homologs, designated as Mnsxl1 and Mnsxl2, were cloned and characterized from the freshwater prawn Macrobrachium nipponense by rapid amplification of cDNA ends. The deduced amino acid sequences of Mnsxl1 and Mnsxl2 showed high sequence homology to the insect Sxl and contained conserved domains in two RNA-binding motifs. Real-ti.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.18.8
- DOI:
- 10.4238/2013.October.18.8
Molecular cloning and site-directed mutagenesis of leucine-based sorting motifs of the porcine invariant chain
Authors: F.Z. Xu, Y. Dai, L.M. Li, X.L. Ding and W.Y. Yu
Invariant chain (Ii) is a transmembrane protein that associates with MHC class II molecules in the endoplasmic reticulum. The cytoplasmic tail of Ii contains two leucine residues able to direct Ii to the endocytic pathway. We obtained the pig Ii gene by RT-PCR. Mutated Ii was prepared via site directed mutagenesis by the PCR Megaprimer method to study the.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.January.4.10
- DOI:
- 10.4238/2013.January.4.10
Cellular responses induced in vitro by pestheic acid, a fungal metabolite, in a gastric adenocarcinoma cell line (PG100)
Authors: J.M.C. Sousa, L.A. Matos, D.F.A. Alc�¢ntara, H.F. Ribeiro, L.S. Santos, M.N. Oliveira, L.C. Brito-Junior, A.S. Khayat, A.C. Guimar�£es, L.A. Cunha, R.R. Burbano and M.O. Bahia
There is a constant search for new cancer treatments that are less aggressive and economically affordable. In this context, natural products extracted from plants, fungi, and microorganisms are of great interest. Pestheic acid, or dihidromaldoxin, is a chlorinated diphenylic ether extracted from the phytopathogenic fungus Pestalotiopsis guepinii (Amphisph.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.1.1
- DOI:
- 10.4238/2013.October.1.1
Screening for feature genes associated with hereditary hemochromatosis and functional analysis with DNA microarrays
Authors: J. Wang, X. Zhou, J. Zhao, Z. Li and X. Li
The aim of this study was to identify feature genes that are associated with hereditary hemochromatosis (HHC; iron overload) in cardiac and skeletal muscle of mice. First, the expression profile GSE9726 was downloaded from Gene Expression Omnibus database which included 12 samples. Then the differentially expressed genes (DEGs) were identified by R langua.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.4.11
- DOI:
- 10.4238/2013.December.4.11
Aberrant promoter methylation of the CD4 gene in peripheral blood cells of mastitic dairy cows
Authors: X.S. Wang, Y. Zhang, Y.H. He, P.P. Ma, L.J. Fan, Y.C. Wang,Y.I. Zhang, D.X. Sun, S.L. Zhang, C.D. Wang, J.Z. Song and Y. Yu
Bovine mastitis is the most common and costly disease of dairy cattle. Cluster of differentiation 4 (CD4) is closely related to the immune response in mastitis. We quantified promoter CpG methylation levels of the CD4 gene in Chinese Holsteins with clinical mastitis (CM) and in healthy controls; these levels were quantitatively detected with bisulfite pyr.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.4.10
- DOI:
- 10.4238/2013.December.4.10
Genetic polymorphisms of LPL and HL and their association with the performance of Chinese sturgeons fed a formulated diet
Authors: Y. He1*, D. Shen1*, X.F. Liang1, R.H. Lu1 and H. Xiao2
It is very important to investigate the reasons for the large individual differences in individual performance of food acceptance when using formulated diets for the successful culture of larvae and juveniles of the Chinese sturgeon Acipenser sinensis. Genetic differences of the mitochondrial control region were investigated by direct sequencing in two gr.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.15.4
- DOI:
- 10.4238/2013.October.15.4