Microbial Genetics
Transcriptomic profiles of Japanese medaka (Oryzias latipes) in response to alkalinity stress
Authors: Z.L. Yao, H. Wang, L. Chen, K. Zhou, C.Q. Ying and Q.F. Lai
Oryzias latipes (Adrianichthyidae), known as Japanese medaka or Japanese killifish, is a small 2-4 cm long fish common in rice paddies in coastal Southeast Asia and is also a popular aquarium fish. It has been widely used as a research model because of its small size and because it is very easy to rear. Alkalinity stress is considered to be one of the maj.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.June.15.2
- DOI:
- 10.4238/2012.June.15.2
Microarray-based gene expression profiles in rabbit retina due to negative pressure suction
Authors: H.X. Zhao, C.M. Niu and W.Y. Guan
We investigated a possible molecular pathogenesis involving retinal ganglion cell apoptosis following transient high intraocular pressure. Changes in the gene expression profiles of the retina were detected via gene chip methodology. Twelve New Zealand white rabbits were randomly assigned to control and 3-min negative pressure suction groups. The control .. Read More»
- Genet. Mol. Res. 11(3):
- 2012.May.18.9
- DOI:
- 10.4238/2012.May.18.9
Differences in numbers of termicins expressed in two termite species affected by fungal contamination of their environments
Authors: P. Xu, M. Shi, R. Lai and X.-X. Chen
Termicin is a defensin-like antimicrobial peptide of termites that has strong antifungal activity. Fifty-six different termicin mRNAs encoding 46 different peptides were amplified and identified from Odontotermes formosanus (Termitidae), a species that inhabits environments with a large variety of microbial fauna. In contrast, only 38 different termicin m.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.May.10.2
- DOI:
- 10.4238/2012.May.10.2
Comparison between modified DNA extraction protocols and commercial isolation kits in grapevine (Vitis vinifera L.)
Authors: M. Akkurt
Various protocols have been developed and used for DNA extraction in grapevine. However, owing to the long duration of the isolation steps in previously developed protocols, researchers have preferred to use isolation kits for studies in recent years. In our study, the DNA yield and purity obtained using six methods - namely three DNA isolation protocols .. Read More»
- Genet. Mol. Res. 11(3):
- 2012.August.13.8
- DOI:
- 10.4238/2012.August.13.8
Microdeletion on 17p11.2 in a Smith-Magenis syndrome patient with mental retardation and congenital heart defect: first report from China
Authors: C. Huang, Y.-F. Yang, H. Zhang, L. Xie, J.-L. Chen, J. Wang, Z.-P. Tan and H. Luo
Smith-Magenis syndrome (SMS) is a rare syndrome with multiple congenital malformations, including development and mental retardation, behavioral problems and a distinct facial appearance. SMS is caused by haploinsufficiency of RAI1 (deletion or mutation of RAI1). We describe an eight-year-old female Chinese patient with multiple malformations, congenital .. Read More»
- Genet. Mol. Res. 11(3):
- 2012.August.13.5
- DOI:
- 10.4238/2012.August.13.5
Fine-scale spatial genetic structure of an endangered marsh herb, Caldesia grandis (Alismataceae)
Authors: X.-L. Yue, J.-M. Chen, Y.-H. Guo and Q.-F. Wang2
The endangered marsh herb, Caldesia grandis, is native to China. We investigated the spatial structure of the genetic variation of three populations of C. grandis using RAPD markers and spatial autocorrelation analysis, based on the method of equal distance interval. A total of 157 individuals were sampled from four patches collected from the region of Hu.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.May.18.10
- DOI:
- 10.4238/2012.May.18.10
Morphological traits and RAPD markers for characterization and identification of minor Spanish olive cultivars from the Extremadura region
Authors: M.C. Parra-Lobato, F.J. Delgado-Martinez and M.C. Gomez-Jimenez
A total of 91 wild olive accessions and 31 olive cultivars growing in the Extremadura region of central-western Spain were analyzed using morphological traits and RAPD markers. We focused on three main and 16 minor Spanish olive cultivars that are recognized as native or local to the Extremadura region. The five arbitrary 10-mer primers tested on the oliv.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.May.10.6
- DOI:
- 10.4238/2012.May.10.6
Genetic diversity of polysporic isolates of Moniliophthora perniciosa (Tricholomataceae)
Authors: L.F.R. Ferreira, K.M.R. Duarte, L.H. Gomes, R.S. Carvalho, G.A. Leal Junior, M.M. Aguiar, R.D. Armas and F.C.A. Tavares
The causal agent of witches’ broom disease, Moniliophthora perniciosa is a hemibiotrophic and endemic fungus of the Amazon basin and the most important cocoa disease in Brazil. The purpose of this study was to analyze the genetic diversity of polysporic isolates of M. perniciosa to evaluate the adaptation of the pathogen from different Brazilian reg.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.July.10.11
- DOI:
- 10.4238/2012.July.10.11
Mapping of HtNB, a gene conferring non-lesion resistance before heading to Exserohilum turcicum (Pass.), in a maize inbred line derived from the Indonesian variety Bramadi
Authors: H. Wang, Z.X. Xiao, F.G. Wang, Y.N. Xiao, J.R. Zhao, Y.L. Zheng and F.Z. Qiu
The gene HtNB confers non-lesion resistance to the fungal pathogen Exserohilum turcicum in maize. To map this gene, we developed two F2 populations, P111 (resistant line) x HuangZao 4 (susceptible line) and P111 x B73 (susceptible). HtNB was located on chromosome 8.07 bin, flanked by MAC216826-4 and umc2218 at distances of 3.3 and 3.4 cM, respectively. Ht.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.July.10.7
- DOI:
- 10.4238/2012.July.10.7
Detection limits of the strip test and PCR for genetically modified corn in Brazil
Authors: V.E. Nascimento, ��.V.R. Von Pinho, R.G. Von Pinho and A.D. do Nascimento J�ºnior
Brazilian legislation establishes a labeling limit for products that contain more than 1% material from genetically modified organisms (GMOs). We assessed the sensitivity of the lateral flow strip test in detection of the GMO corn varieties Bt11 and MON810 and the specificity and sensitivity of PCR techniques for their detection. For the strip test, the G.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.June.27.2
- DOI:
- 10.4238/2012.June.27.2