Microbial Genetics
Expression of the bovine papillomavirus type 1, 2 and 4 L1 genes in the yeast Pichia pastoris
Authors: A.L.S. Jesus, F.C. Mariz, H.M. Souza, M.N. Cordeiro, E.C. Coimbra, M.C.G. Leit�£o, L.M. Nascimento, R.C. Stocco, W. Be�§ak and A.C. Freitas
Papillomaviruses are known to cause benign or malignant lesions in various animals. In cattle, bovine papillomavirus (BPV) is the etiologic agent of papillomatosis and neoplasia of the upper gastrointestinal tract and urinary bladder. Currently, there are no standard diagnostic tests or prophylactic vaccines. Protection against papillomavirus infection is.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.July.19.2
- DOI:
- 10.4238/2012.July.19.2
Genetic structure in fragmented populations of Solanum lycocarpum A. St.-Hil. with distinct anthropogenic histories in a Cerrado region of Brazil
Authors: T.M. Moura, K. Martins, P.S. Sujii, A.M. Sebbenn and L.J. Chaves
Solanum lycocarpum is a woody tree widely distributed in the Cerrado that reaches high population densities in disturbed environments. We examined the genetic diversity and population differentiation of six S. lycocarpum populations with different degrees of human disturbance in order to determine if they are negatively affected by anthropogenic activity... Read More»
- Genet. Mol. Res. 11(3):
- 2012.July.10.16
- DOI:
- 10.4238/2012.July.10.16
Isolation and molecular characterization of a novel strain of Bacillus with antifungal activity from the sorghum rhizosphere
Authors: S.C. Martinez-Absalon, Ma. del C. Orozco-Mosqueda, M.M. Martinez-Pacheco, R. Farias-Rodriguez, M. Govindappa and G. Santoyo
We looked for bacterial strains with antifungal activity in the sorghum rhizosphere. A prescreening procedure to search for hemolytic activity among the isolated strains allowed us to detect good fungitoxic activity in a bacterial isolate that we named UM96. This bacterial isolate showed strong growth inhibition in bioassays against the pathogens Diaporth.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.July.10.15
- DOI:
- 10.4238/2012.July.10.15
Effect of radiotherapy on the treatment of patients with extensive stage small cell lung cancer
Authors: C. Wu, T. Wang, J. Wang, B. Qu, H. Wang and Y. Hu
Small cell lung cancer (SCLC) is an aggressive disease, in which more than 80% of patients present distant metastases at the time of first diagnosis. Chemotherapy is considered as the main treatment of extensive disease of SCLC (ED-SCLC), while the role of radiotherapy in the treatment of ED-SCLC is controversial. A case-control study was undertaken of pa.. Read More»
- Genet. Mol. Res. 13(4):
Characteristics of protein variants in trichlorphon-resistant Bactrocera dorsalis (Diptera; Tephritidae) larvae
Authors: T. Jin, L. Zeng, Y.-Y. Lin, Y.-Y. Lu and G.-W. Liang
Functional proteins in larvae of Bactrocera dorsalis, a major fruit pest, play a central role in their resistance to organophosphorus insecticides. Changes in proteins in B. dorsalis larvae after trichlorphon treatment may have a role in the resistance response to trichlorphon. We analyzed 14 protein spots of crude proteins from B. dorsalis larvae post-tr.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.July.10.12
- DOI:
- 10.4238/2012.July.10.12
Pyrroloquinoline quinone rescues hippocampal neurons from glutamate-induced cell death through activation of Nrf2 and up-regulation of antioxidant genes
Authors: Q. Zhang, M. Ding, X.R. Gao and F. Ding
Pyrroloquinoline quinone (PQQ) has been shown to protect primary cultured hippocampal neurons from glutamate-induced cell apoptosis by scavenging reactive oxygen species (ROS) and activating phosphatidylinositol-3-kinase (PI3K)/Akt signaling. We investigated the downstream pathways of PI3K/Akt involved in PQQ protection of glutamate-injured hippocampal ne.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.June.27.3
- DOI:
- 10.4238/2012.June.27.3
Nested polymerase chain reaction amplification and sequencing analysis of the light-chain and heavy-chain variable regions in the influenza A H1N1 virus hemagglutinin monoclonal antibody gene
Authors: H.J. Li, C.Y. Guo, J.Y. Sun, L.J. Sun, P.H. Zhao, L. Hu, Y. Li and J. Hu
The nested polymerase chain reaction (PCR) method was used for the amplification of the influenza A H1N1 virus hemagglutinin monoclonal antibody light-chain and heavy-chain genes. Sequence analysis of the obtained genes was then used to identify common cloning methods of the mouse immunoglobulin-kappa (Igκ) light-chain a.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.June.11.1
- DOI:
- http://dx.doi.org/10.4238/2014.June.11.1
Diagnostic value of cytological and microbiological methods in cryptococcal meningitis
Authors: M. Zhang1*, J.C. Li2*, H. Lin1, W. Zhang2, M. Lin1, L. Wu1, W. Liu3, J.S. Mu1, J.X. Ye1 and X.P. Cui1
The aim of this study was to investigate diagnostic methods for cryptococcal meningitis (CM). A retrospective analysis was conducted for 31 patients with CM confirmed by etiologic detection of cerebrospinal fluid in our hospital in the past 5 years. Nineteen cases in 31 patients were confirmed with CM in the first diagnosis, with a misdiagnosis rate of 38.. Read More»
- Genet. Mol. Res. 13(4):
Arm-Gal4 inheritance influences development and lifespan in Drosophila melanogaster
Authors: F.A. Slade and B.E. Staveley
The UAS-Gal4 ectopic expression system is a widely used and highly valued tool that allows specific gene expression in Drosophila melanogaster. Yeast transcription factor Gal4 can be directed using D. melanogaster transcriptional control elements, and is often assumed to have little effect on the organism. By evaluation of the.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.19.22
- DOI:
- 10.4238/2015.October.19.22
Functional analysis of differentially expressed genes associated with glaucoma from DNA microarray data..
Authors: Y. Wu, W.D. Zang and W. Jiang
Microarray data of astrocytes extracted from the optic nerves of donors with and without glaucoma were analyzed to screen for differentially expressed genes (DEGs). Functional exploration with bioinformatic tools was then used to understand the roles of the identified DEGs in glaucoma. Microarray data were downloaded from the Gene Expression Omnibus (GEO).. Read More»
- Genet. Mol. Res. 13(4):