Microbial Genetics
Determination of genetic variation among chickpea genotypes and their F1 crosses with RAPD markers
Authors: K. Mahmood, W. Nazeer, M. Naeem, S. Freed, M.A. Khan, G. Abbas, M. Iqbal, A.R. Kulachi, S.A. Sajid, M.A. Sadiq and M. Saleem
The intensity of genetic diversity amongst chickpea genotypes and their crosses is unknown. The current study investigated the genetic diversity of chickpea genotypes and their F1 crosses by using random amplified polymorphic DNA (RAPD) markers. We assessed the variation among 6 chickpea genotypes and 15 F1 crosses with the RAPD markers. The 6 parents and.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.10.1
- DOI:
- 10.4238/2013.April.10.1
De novo assembly, functional annotation, and marker development of Asian pear (Pyrus pyrifolia) fruit transcriptome through massively parallel sequencing
Authors: J.F. Li, Z. Gao, Y.S. Lou, M. Luo, S.R. Song, W.P. Xu, S.P. Wang and C.X. Zhang
This study investigated the Asian pear transcriptome using the RNA-Seq normalized fruit cDNA library to create a transcriptomic resource for unigene and marker discovery. Following the removal of lowquality reads, 127,085,054 trimmed reads were assembled de novo to yield 37,649 non-redundant unigenes with an average length of 599 bp. Alternative splicing .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.23.22
- DOI:
- 10.4238/2015.December.23.22
Expression of recombinant myostatin propeptide pPIC9K-Msp plasmid in Pichia pastoris
Authors: W. Du, J. Xia, Y. Zhang, M.J. Liu, H.B. Li, X.M. Yan, J.S. Zhang, N. Li, Z.Y. Zhou and W.Z. Xie
Myostatin propeptide can inhibit the biological activity of myostatin protein and promote muscle growth. To express myostatin propeptide in vitro with a higher biological activity, we performed codon optimization on the sheep myostatin propeptide gene sequence, and mutated aspartic acid-76 to alanine based on the codon usage bias of Pichia pastoris and th.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.23.29
- DOI:
- 10.4238/2015.December.23.29
DGGE and multivariate analysis of a yeast community in spontaneous cocoa fermentation process
Authors: A.C.R. Ferreira, E.L.S. Marques, J.C.T. Dias and R.P. Rezende
Cocoa bean is the main raw material used in the production of chocolate. In southern Bahia, Brazil, cocoa farming and processing is an important economic activity. The fermentation of cocoa is the processing stage that yields important chocolate flavor precursors and complex microbial involvement is essential for this process. In this study, PCR-denaturin.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.23.34
- DOI:
- 10.4238/2015.December.23.34
Molecular cloning and expression analysis of a novel BCCP subunit gene from Aleurites moluccana
Authors: W.Y. Xuan, Y. Zhang, Z.Q. Liu, D. Feng and M.Y. Luo
Aleurites moluccana L. is grown as a roadside tree in southern China and the oil content of its seed is higher than other oil plants, such as Jatropha curcas and Camellia oleifera. A. moluccana is considered a promising energy plant because its seed oil could be used to produce biodiesel and bio-jet fuel. In addition, the bark, leaves, and kernels of A. m.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.19.27
- DOI:
- 10.4238/2015.August.19.27
Cloning and characterization of ADP-glucose pyrophosphorylase small subunit gene in Cyperus esculentus (yellow nutsedge)
Authors: C. Cheng, J. Hu, Y. Zhi, J.J. Su, X.K. Zhang and B.Q. Huang
ADP-glucose pyrophosphorylase (ADPGlcPPase) controls the first committed step of starch synthesis by catalyzing the biosynthesis of ADP-glucose from glucose-phosphate and ATP. It is a tetrameric protein consisting of two small and two large subunits. The small subunits have a catalytic function, while the large subunits regulate the enzyme activity. Cyper.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.23.18
- DOI:
- 10.4238/2015.December.23.18
DNA barcoding and phylogenetic relationships of genera Picoides and Dendrocopos (Aves: Picidae)
Authors: Z.H. Huang, F.Y. Tu and X.J. Liao
Picoides and Dendrocopos are two closely related genera of woodpeckers (family Picidae), and members of these genera have long been the subjects of phylogenetic debate. Mitochondrial cytochrome c oxidase subunit I (COI) is a powerful marker for the identification and phylogenetic study of animal species. In the present study, we analyzed the COI barcodes .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.23.24
- DOI:
- 10.4238/2015.December.23.24
Can Spirulina maxima reduce the mutagenic potential of sibutramine?
Authors: R.P. Araldi, N.P. Santos, T.B. Mendes, L.B. Carvalho, E.T. Ito, P.L. de-S�¡-J�ºnior and EB Souza
The worldwide obesity pandemic requires the use of anti-obesity drugs. Sibutramine is an anti-obesity drug that has been used worldwide but is indiscriminately consumed in Brazil. Several studies have demonstrated that sibutramine promotes weight loss and weight maintenance, but several side effects have been associated with its systematic consumption. Fo.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.23.33
- DOI:
- 10.4238/2015.December.23.33
Isolation and characterization of microsatellite loci in Quercus fabri (Fagaceae)
Authors: Z.Z. Xiao, W.W. Chen, W. Bao, R. Wang and Y.Y. Li
Quercus fabri is a pioneer species of secondary succession in evergreen broadleaved forests in China. In this study, we isolated and developed 12 polymorphic and 2 monomorphic microsatellite loci for Q. fabri using the biotin-streptavidin capture method. We characterized 12 polymorphic loci in 52 individuals from two populatio.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028322
- DOI:
- 10.4238/gmr.15028322
Prospective uses of recombinant Lactococcus lactis expressing both listeriolysin O and mutated internalin A from Listeria monocytogenes as a tool for DNA vaccination
Authors: M.S.P. De Azevedo, C. Santos Rocha, V.B. Pereira, A.F. De Oliveira Junior, C.S. De Sousa, V. Azevedo, J.G. LeBlanc, J.M. Chatel and A. Miyoshi
In this study, Lactococcus lactis was engineered to express mutated internalin A on its surface and to secrete large amounts of listeriolysin O (LLO) in order to improve its potential as a vehicle for DNA vaccination. Western blotting experiments demonstrated that the bacterium expressed LLO in both the cytoplasmic and extracellular compartments, with hig.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.23.36
- DOI:
- 10.4238/2015.December.23.36