Microbial Genetics
Detection of soluble expression and in vivo interactions of the inner membrane protein OppC using green fluorescent protein
Authors: Q.J. Xiang, J.F. Zhai, M. Zhang and B. Zhang
In this study, the in vivo interaction system of oligopeptide permease (Opp) proteins was analyzed, and a high expression system of inner membrane protein OppC was constructed by flexible usage of the green fluorescent protein (GFP). The Escherichia coli OppC gene, which encodes a transmembrane component of oligopeptide transporter, was cloned into differ.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.22.8
- DOI:
- 10.4238/2015.December.22.8
Comparative transcriptome analysis of Nile tilapia (Oreochromis niloticus) in response to alkalinity stress
Authors: Y. Zhao, J. Wang, J. Thammaratsuntorn, J.W. Wu, J.H. Wei, Y. Wang, J.W. Xu and J.L. Zhao
To identify genes of potential importance to alkalinity tolerance, RNA sequencing (RNA-Seq) was performed to survey gill transcriptome profiles from freshwater (FW) and alkaline water (AW) exposed Nile tilapia (Oreochromis niloticus). A total of 22,724,036 (AW)/16,461,040 (FW) single-end reads were generated in which 20,304,348 (AW)/14,681,290 (FW) reads .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.22.16
- DOI:
- 10.4238/2015.December.22.16
Expression patterns of the STAG gene in intact and regenerating planarians (Dugesia japonica)
Authors: Z.Q. Yuan, B.S. Zhao and J.Y. Zhang
We examined the spatial and temporal expression of the planarian Dugesia japonica STAG-related gene (DjStag), in both intact and regenerating planarians, by whole-mount in situ hybridization and relative quantitative real-time PCR. The first localized transcripts of DjStag were detected in the blastemas three days after amputa.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr1042
- DOI:
- 10.4238/vol10-1gmr1042
Effective sample selection for classification of pre-miRNAs
Authors: K. Han
To solve the class imbalance problem in the classification of pre-miRNAs with the ab initio method, we developed a novel sample selection method according to the characteristics of pre-miRNAs. Real/pseudo pre-miRNAs are clustered based on their stem similarity and their distribution in high dimensional sample space, respective.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr1054
- DOI:
- 10.4238/vol10-1gmr1054
A novel molecular marker for the study of Neotropical cichlid phylogeny
Authors: T.M.C. Fabrin, L.S. Gasques, S.M.A.P. Prioli and A.J. Prioli
The use of molecular markers has contributed to phylogeny and to the reconstruction of species’ evolutionary history. Each region of the genome has different evolution rates, which may or may not identify phylogenetic signal at different levels. Therefore, it is important to assess new molecular markers that can be used for phylogenetic reconstructi.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.22.39
- DOI:
- 10.4238/2015.December.22.39
Morphological and genetic variation of the pine shoot tunnel beetle Placusa pinearum (Staphylinidae) in China
Authors: J.-Y. Gao, B.-Z. Ji and S.-W. Liu
Placusa pinearum, a newly described beetle species found living in pine shoot tunnels bored by the pine tip moth, Dioryctria rubella (Pyralidae), has potential as a vector to transport biological agents for controlling this moth pest of pine trees because of the high degree of niche overlap (co-occurrence) between them. In order to help determine the suit.. Read More»
- Genet. Mol. Res. 12(2):
Age-dependent expression of the BPI gene in Sutai piglets
Authors: J. Zhu, C. Zi, Z.C. Wu, L. Liu, X.R. Zheng, X.M. Su, G.Q. Zhu, X.G. Huang, S.L. Wu and W.B. Bao
We compared and analyzed expression of the BPI gene of Sutai piglets ranging from newborn to post-weaning days 8, 18, 30, and 35 by the real-time PCR method, in order to determine if it is involved in protection against disease caused by ETEC F18. There was a significant difference between 18- and 35-day expression in the jejunum. There were also signific.. Read More»
- Genet. Mol. Res. 12(2):
- 2012.April.12.1
- DOI:
- 10.4238/2012.April.12.1
Complete mitochondrial genome of the Southern catfish (Silurus meridionalis Chen) and Chinese catfish (S. asotus Linnaeus): Structure, phylogeny, and intraspecific variation
Authors: Q.R. Wang, C. Xu, C.R. Xu and R.J. Wang
The complete mitochondrial genome of the Southern catfish (Silurus meridionalis) and the Chinese catfish (S. asotus), was determined using the long and accurate polymerase chain reaction (LA-PCR) method. The mitochondrial DNA nucleotide sequences of S. meridionalis and S. asotus were compared with those of 47 other catfish species in the same order. The t.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.23.7
- DOI:
- 10.4238/2015.December.23.7
Expression of GFP can influence aging and climbing ability in Drosophila
Authors: R.M.S. Mawhinney and B.E. Staveley
Green fluorescent protein (GFP) is widely used as a reporter transgene in a variety of organisms. Some of the advantages of using GFP include non-invasive visualization of biological events and/or tissues in live specimens and its benign nature. When GFP is expressed throughout the organism, in neurons and eyes, lifespan and c.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr1023
- DOI:
- 10.4238/vol10-1gmr1023
An outbreak of Candida parapsilosis fungemia among preterm infants
Authors: W. Guo, H.F. Gu, H.G. Zhang, S.B. Chen, J.Q. Wang, S.X. Geng, L. Li, P. Liu, X. Liu, Y.R. Ji, S.W. Li and L. Yang
In this article, we describe the first outbreak of Candida parapsilosis fungemia in our hospital. We examined a cluster of four nosocomial cases of C. parapsilosis fungemia that occurred in the neonatal intensive care unit (NICU) of the Affiliated Xingtai People’s Hospital of Hebei Medical University over a two-week period. We ascertained patient pa.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.23.13
- DOI:
- 10.4238/2015.December.23.13