Microbial Genetics
Association of the PPAR�³2 Pro12Ala polymorphism with increased risk of cardiovascular diseases
Authors: Y. Li, J. Zhu and J.Q. Ding
This meta-analysis investigated the correlation between the PPARγ2 Pro12Ala polymorphism and cardiovascular disease (CVD). Electronic database and manual searches were conducted to retrieve studies published relevant to the PPARγ2 Pro12Ala polymorphism and CVD. Rigorous inclusion and exclusion criteria were employed for selection of high-quali.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.15
- DOI:
- 10.4238/2015.December.28.15
Thymidylate synthase and methylenetetrahydrofolate reductase gene polymorphisms and gastric cancer susceptibility in a population of Northern Brazil
Authors: M.D. Ara�ºjo, B.N. Borges, S. Rodrigues-Antunes, R.M.R. Burbano and M.L. Harada
The folate metabolic pathway, which is involved in DNA synthesis and methylation, is associated with individual susceptibility to several diseases, including gastric tumors. In this study, we investigated four polymorphisms [thymidylate synthase enhancer region, single nucleotide polymorphism thymidylate synthase 5' (TS5'), TS3' untranslated region, and m.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.21.6
- DOI:
- 10.4238/2015.August.21.6
Effect of Bifidobacterium on the mRNA expression levels of TRAF6, GSK-3�², and microRNA-146a in LPS-stimulated rat intestinal epithelial cells
Authors: W. Zhou, Y. Yuan, J. Li, W.M. Yuan, L.G. Huang and S.W. Zheng
We investigated the effect of inactivated Bifidobacterium on the mRNA expression of TRAF6, GSK-3β, and microRNA-146a in lipopolysaccharide (LPS)-stimulated rat small intestinal epithelial cells (IEC-6s). IEC-6s were randomly divided into an LPS group, a culture supernatant group, and an inactivated bacteria group. After stimulation with LPS for 5 h, .. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.21.11
- DOI:
- 10.4238/2015.August.21.11
Molecular cloning and expression analysis of a pearl oyster (Pinctada martensii) heat shock protein 90 (HSP90)
Authors: H.Y. Liang, Z.X. Wang, Q.N. Lei, R.L. Huang, Y.W. Deng, Q.H. Wang, Y. Jiao and X.D. Du
Heat shock protein 90 (HSP90) is an important molecular chaperone required for proper folding of cellular proteins, and thus, it plays an essential role in protecting cells from damage during stress. In this study, an HSP90 cDNA designated PmHSP90 was cloned from the mantle tissue of the pearl oyster Pinctada martensii using reverse transcription polymera.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.27
- DOI:
- 10.4238/2015.December.28.27
Molecular tracing of white muscardine in Asian corn borer using inter-simple sequence repeat (ISSR) analysis
Authors: B.J. Hu, L.N. Xu, Z.Y. Zhou, F. Hu, F.G. Luan, X. Chen and Z.Z. Li
Beauveria bassiana is a soil fungus that parasitizes arthropod species, and is used to control the Asian corn borer in Northeast China. In this study, B. bassiana was investigated in Xiaoxian County and Baicheng City, and the results were compared with those of Gongzhuling City, where the fungus was not applied. Using the inter-simple sequence repeat (ISS.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.21
- DOI:
- 10.4238/2015.December.28.21
Association between PAI-1 polymorphisms and plasma PAI-1 level with sepsis in severely burned patients
Authors: Y.F. Chi, J.K. Chai, Y.M. Yu H.M. Luo, Q.X. Zhang and R. Feng
We investigated the association between plasminogen activator inhibitor-1 (PAI-1) polymorphisms and plasma PAI-1 level with sepsis in severely burned patients. A total of 182 patients with burn areas lager than 30% of the body surface area were enrolled in this study. Peripheral blood samples were obtained from 103 patients with sepsis (sepsis group) and .. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.21.15
- DOI:
- 10.4238/2015.August.21.15
Role of autoantibodies to various Ro60 epitopes in the decrease of lymphocytes seen in systemic lupus erythematosus and primary Sj�¶grenâ��s syndrome
Authors: Z.W. Shuai, Y. Huang, L. Zhang, J. Cai and M. Li
We investigated the roles of autoantibodies to different Ro60 epitopes in lymphopenia in systemic lupus erythematosus (SLE) and primary Sjögren’s syndrome (pSS). We recruited 16 patients with SLE, 14 with pSS, and 10 healthy controls; all were female. Patients had active disease, had not received glucocorticoid or immunosuppressants for at leas.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.21.17
- DOI:
- 10.4238/2015.August.21.17
Superoxide radical-generating compounds activate a predicted promoter site for paraquat-inducible genes of the Chromobacterium violaceum bacterium in a dose-dependent manner
Authors: J.E. Gabriel, E.P. Guerra-Slompo, E.M. de Souza, F.A.L. de Carvalho, H.M.F. Madeira and A.T.R. de Vasconcelos
The purpose of the present study was to functionally evaluate the influence of superoxide radical-generating compounds on the heterologous induction of a predicted promoter region of open reading frames for paraquat-inducible genes (pqi genes) revealed during genome annotation analyses of the Chromobacterium violaceum bacterium. A 388-bp fragment correspo.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.21.20
- DOI:
- 10.4238/2015.August.21.20
Molecular cloning and expression vector construction of bovine TRIM28
Authors: X. Ma, Z.C. Zhai, M.L. Zhang, B.H. Song, Y.R. Zhu, S.B. Yang, X.Q. Dong, L.Y. Su, C.F. Wang, H.X. Ma and W.M. Luan
The bovine TRIM28 gene was amplified from ovary tissue by using RT-PCR. The TRIM28 gene was inserted into the eukaryotic expression vector pIRES2-EGFP and transfected into bovine fetal fibroblasts by using Lipofectamine 3000. TRIM28 mRNA and protein were detected by fluorescence microscope and western blotting. The results sho.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028793
- DOI:
- 10.4238/gmr.15028793
Expression profiles of MMP-1 and TIMP-1 in lumbar intervertebral disc degeneration
Authors: B. Deng, J.Z. Ren, X.Q. Meng, C.G. Pang, G.Q. Duan, J.X. Zhang, H. Zou, H.Z. Yang and J.J. Ji
Lumbar intervertebral disc degeneration (IDD) is a common clinical pathology and has become a focus for research in recent years. Matrix metalloproteinases (MMPs) are enzymes responsible for the degradation of almost all extracellular matrix proteins (ECM). The over-expression of MMPs or tissue inhibitors of metalloproteinases (TIMPs) may disrupt the dyna.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.29.16
- DOI:
- 10.4238/2015.December.29.16