Medical Genetics
Molecular cloning, overexpression, purification, and sequence analysis of the giant panda (Ailuropoda melanoleuca) ferritin light polypeptide
Authors: L. Fu, Y.L. Hou, X. Ding, Y.J. Du, H.Q. Zhu, N. Zhang and W.R. Hou
The complementary DNA (cDNA) of the giant panda (Ailuropoda melanoleuca) ferritin light polypeptide (FTL) gene was successfully cloned using reverse transcription-polymerase chain reaction technology. We constructed a recombinant expression vector containing FTL cDNA and overexpressed it in Escherichia coli using pET28a plasmi.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038593
- DOI:
- 10.4238/gmr.15038593
Preliminary study on total protein extraction methods from Enterococcus faecalis biofilm
Authors: W. Chen, J. Liang, Z. He and W. Jiang
Enterococcus faecalis is the major pathogen of post-endodontic disease and refractory periapical periodontitis, and recent research on this species has focused on its pathogenicity. E. faecalis most often causes disease in the form of a biofilm, and total protein expression shows a strong association with its virulence. Theref.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038988
- DOI:
- 10.4238/gmr.15038988
Locally linear embedding and neighborhood rough set-based gene selection for gene expression data classification
Authors: L. Sun, J.-C. Xu, W. Wang and Y. Yin
Cancer subtype recognition and feature selection are important problems in the diagnosis and treatment of tumors. Here, we propose a novel gene selection approach applied to gene expression data classification. First, two classical feature reduction methods including locally linear embedding (LLE) and rough set (RS) are summar.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038990
- DOI:
- 10.4238/gmr.15038990
Building the SeqChromMM Markov property atlas of the human genome by analyzing the 200-bp units of the 15 different chromatin regions of ENCODE
Authors: K.-E. Lee and H.-S. Park
We analyzed the publicly available ChromHMM BED files of the ENCODE project and tested the Markov properties of the different chromatin states in the human genome. Nucleotide frequency profiles of regional chromatin segmentations were analyzed, and Markov chains were built to detect Markov properties in the chromatin states of different ChromHMM regions. .. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038992
- DOI:
- 10.4238/gmr.15038992
Nanofibers promote HepG2 aggregate formation and cellular function
Authors: W.T. Su, Y.J. Liu and T.Y. Huang
Formation of hepatocyte spheroids is a necessary strategy for increasing liver-specific function in vitro. In this study, HepG2 cells showed good viability when grown on a polylactic acid-chitosan (PLA-CS) nanofiber and aggregated to form multicellular spheroids on the PLA-CS nanofibers with a diameter of approximately 100-200 mm in 5 days of culture, whe.. Read More»
- Genet. Mol. Res. 15(3):
Relationship between EPHX2 gene polymorphisms and essential hypertension in Uygur, Kazakh, and Han
Authors: X.L. Zhu, L. Wang, Z. Wang, S.Z. Chen, W.Q. Zhang and M.M. Ma
We investigated the association between rs751141 polymorphisms in the EPHX2 gene and essential hypertension in Uygur, Kazakh, and Han subjects in Xinjiang, China. A total of 302 essential hypertensive patients in Uygur, 267 in Kazakh, and 368 in Han, as well as 323 normotensive controls in Uygur, 284 in Kazakh, and 348 in Han were enrolled in this study. .. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.15.11
- DOI:
- http://dx.doi.org/10.4238/2015.April.15.11
Transcriptome sequencing and analysis of the coconut leaf beetle, Brontispa longissima
Authors: W. Yan, L. Liu, C.X. Li, S.C. Huang, Z.L. Ma, W.Q. Qin and Z.Q. Peng
The coconut leaf beetle, Brontispa longissima, is a destructive pest of palm plants. Although its ecological and biological characteristics are well understood, its genetic information remains largely unknown. To advance our understanding of its molecular basis of biology and ecology, we sequenced and analyzed its whole transcriptome by using high-through.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.28.2
- DOI:
- 10.4238/2015.July.28.2
Adaption of SYBR Green-based reagent kit for real-time PCR quantitation of GC-rich DNA
Authors: G.J. Chang, H.M. Seyfert and X.Z. Shen
In the mammalian genome, approximately 50% of all genes are controlled by promoters with high GC contents. Analyzing the epigenetic mechanisms regulating their expression is difficult. Hence, we examined a method for stable quantification of such GC-rich DNA sequences. Quantification of DNA during real-time PCR is often based on reagent kits containing th.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.28.20
- DOI:
- 10.4238/2015.July.28.20
Lack of association between an insertion/deletion polymorphism in IL1A and risk of colorectal cancer
Authors: H. Yan, R. Sun, X. Pan, Z. Li, X. Guo and L. Gao
Previous study has shown that miR-122, miR-378, and their target gene IL1A may play crucial roles in the tumorigenesis of colorectal cancer (CRC). An insertion/deletion polymorphism(rs3783553 TTCA/-) in the 3' untranslated region of IL1A has been identified as a contributing factor to the risk of developing several cancers. The aim of this study was to ev.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.28.17
- DOI:
- 10.4238/2015.July.28.17
Correlation of MSH3 polymorphisms with response and survival in advanced non-small cell lung cancer patients treated with first-line platinum-based chemotherapy
Authors: X.-L. Xu, Y.-L. Yao, W.-Z. Xu, J.-G. Feng and W.-M. Mao
Mismatch repair (MMR) genes, as well as the nucleotide excision repair genes, play an important role in removing cisplatin-DNA adducts, and the mutation of MMR genes in tumors can lead to a decreased response to platinum-based therapies. We examined MutS homolog 3 (MSH3), a mismatch repair gene, and whether polymorphisms of MSH3 were associated with respo.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.15.16
- DOI:
- http://dx.doi.org/10.4238/2015.April.15.16