Microbial Genetics
Cloning and transformation analysis of isoflavone synthase gene into Minshan Trifolium pratense
Authors: H.H. Hu, C.Q. Jing, R. Liu, W.D. Li and H.G. Feng
The aim of this study was to clone the isoflavone synthase (IFS) gene and establish the recombinant Minshan Trifolium pratense. The IFS gene was cloned from the callus of Minshan T. pratense using reverse transcription-polymerase chain reaction. The plant expression vector pRI101-AN-IFS was constructed and introduced into Agrobacterium tumefaciens strain .. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.10.9
- DOI:
- 10.4238/2015.August.10.9
Simple sequence repeat-based analysis of the genetic diversity and population genetic structure of populations of Siniperca chuatsi
Authors: C.F. Sun, X. Ye, Y.Y. Tian and J.J. Dong
In order to provide genetic information for the selective breeding of Siniperca chuatsi, 14 microsatellite DNA loci were used to evaluate the genetic diversity and structure of four farmed populations and one wild population in China. The four cultivated populations were Foshan (FS), Jiangmen (JM), Nanjing (NJ), and Hongze Lake (HZL), and the wild populat.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.10.15
- DOI:
- 10.4238/2015.August.10.15
Analysis of alterations to the transcriptome of Loquat (Eriobotrya japonica Lindl.) under low temperature stress via de novo sequencing
Authors: R.G. Gong, J. Lai, W. Yang, M.A. Liao, Z.H. Wang and G.L. Liang
Loquat (Eriobotrya japonica Lindl.), which originates from the cooler hill regions of southwestern China, is a typical subtropical evergreen tree. Loquat is one of the most important economic crops in China, but the available genomic information is very limited. Here, we present the first deep transcriptomic analysis of loquat. De novo assembly generated .. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.14.6
- DOI:
- 10.4238/2015.August.14.6
Investigation of ABCB1 gene polymorphism with colchicine response in Beh�§etâ��s disease
Authors: H. Saricaoglu, M. Yilmaz, M. Karkucak, H.Z.Y. Ozturk, T. Yakut, T. Gulten, E.B. Baskan, K. Aydogan and K. Dilek
Colchicine is commonly used in the treatment of Behçet’s disease. However, some patients are unresponsive to colchicine treatment. Adenosine triphosphate-binding cassette subfamily B member 1 (ABCB1) transports colchicine out of cells. We investigated a possible association of C3435T polymorphism of the ABCB1 (MDR.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr824
- DOI:
- 10.4238/vol10-1gmr824
OsMYB511 encodes a MYB domain transcription activator early regulated by abiotic stress in rice
Authors: P. Huang, H. Chen, R. Mu, X. Yuan, H.S. Zhang and J. Huang
The MYB-domain proteins exist universally across diverse organisms and regulate numerous processes during the plant life cycle. In the present research, a full-length MYB gene OsMYB511 was identified from rice seedlings through microarray data. Induction of OsMYB511 by cold stress was dramatic in japonica cultivar Jiucaiqing as compared to indica IR26. In.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.14.14
- DOI:
- 10.4238/2015.August.14.14
Amiloride, a urokinase-type plasminogen activator receptor (uTPA) inhibitor, reduces proteinurea in podocytes
Authors: L.B. Xu, N. Chi and W. Shi
This study examined the mechanism of action of amiloride, a urokinase-type plasminogen activator receptor inhibitor, in lowering proteinuria. Podocytes were resuscitated to allow for their proliferation and were observed for morphological changes. In the in vitro experiment, control, lipopolysaccharide, and lipopolysaccharide + amiloride groups were estab.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.14.15
- DOI:
- 10.4238/2015.August.14.15
Assessment of genotoxicity of waterpipe smoking using 8-OHdG biomarker
Authors: M. Azab, O.F. Khabour, K.H. Alzoubi, S.A.H. Mahmoud, M. Anabtawi and M. Quttina
Waterpipe tobacco smoking is increasing in popularity, particularly among young adults. This popularity is related to the lack knowledge regarding the health effects of waterpipe smoking. In this study, we examined the genotoxicity of waterpipe smoking using an 8-hydroxy deoxyguanosine (8-OHdG) assay. Genotoxicity was evaluated in the saliva, urine, and s.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.14.18
- DOI:
- 10.4238/2015.August.14.18
Pathway-based network analysis of myeloma tumors: monoclonal gammopathy of unknown significance, smoldering multiple myeloma, and multiple myeloma
Authors: L. Dong, C.Y. Chen, B. Ning, D.L. Xu, J.H. Gao, L.L. Wang, S.Y. Yan and S. Cheng
Although many studies have been carried out on monoclonal gammopathy of unknown significances (MGUS), smoldering multiple myeloma (SMM), and multiple myeloma (MM), their classification and underlying pathogenesis are far from elucidated. To discover the relationships among MGUS, SMM, and MM at the transcriptome level, differentially expressed genes in MGU.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.14.20
- DOI:
- 10.4238/2015.August.14.20
Lack of association between potential prothrombotic genetic risk factors and arterial and venous thrombosis
Authors: F.C.G. Evangelista, D.R.A. Rios, D.D. Ribeiro, M.G. Carvalho, L.M.S. Dusse, A.P. Fernandes and A.P. Sabino
Recent studies have shown an association between thrombosis and factor VII (FVII), tissue factor (TF), and angiotensin-converting enzyme (ACE). This suggests that individuals with FVII-402 G/A, FVII-401 G/T, TF+5466 A/G, and ACE-287 insertion/deletion (I/D) polymorphisms present an increased risk of venous thrombosis, heart disease, and ischemic stroke co.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.14.21
- DOI:
- 10.4238/2015.August.14.21
Mechanism of rat osteosarcoma cell apoptosis induced by a combination of low-intensity ultrasound and 5-aminolevulinic acid in vitro
Authors: Y.N. Li, Q. Zhou, B. Yang, Z. Hu, J.H. Wang, Q.S. Li and W.W. Cao
We investigated the killing effect of low-intensity ultrasound combined with 5-aminolevulinic acid (5-ALA) on the rat osteosarcoma cell line UMR-106. Logarithmic-phase UMR-106 cells were divided into a control group, ultrasound group and 5-ALA group. The cell apoptotic rate, production of reactive oxygen species, and the change in mitochondrial membrane p.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.14.23
- DOI:
- 10.4238/2015.August.14.23