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Microbial Genetics

Microbial Genetics   Research Article

Cloning and polymorphism analysis of the 2-methyl-6-phytyl-1,4-benzoquinol methyltransferase gene (VTE3) in Arachis hypogaea, A. duranensis, and A. ipa�«nsis

Authors: F.Z. Liu, A.Q. Guo and Y.S. Wan

One of the critical enzymes involved in vitamin E biosynthesis in plants is 2-methyl-6-phytyl-1,4-benzoquinol methyltransferase (MPBQ MT). The full-length VTE3 cDNA (designated rVTE3-1 and -2) encoding MPBQ MT and the full-length DNA of VTE3 (designated gVTE3-1 and -2) were isolated from cultivated peanuts (Arachis hypogaea). The full-length DNA of VTE3 (.. Read More»

Genet. Mol. Res. 12(2):
2013.January.4.5
DOI:
10.4238/2013.January.4.5
Microbial Genetics   Research Article

Karyotype studies on populations of two Hypochaeris species (H. catharinensis and H. lutea), Asteraceae, endemics to southern Brazil

Authors: F.G. Fiorin, P.M. Ruas, M.A. Ortiz, E. Urtubey, N.I. Matzenbacher and C.F. Ruas

Hypochaeris is an excellent system for studying different modes of chromosome evolution in plants. We carried out a cytogenetic analysis on populations of 2 Hypochaeris species, comprising 10 populations of H. catharinensis and 5 of H. lutea, to assess possible changes on chromosome organization in this interesting genus. Conventional Feulgen staining and.. Read More»

Genet. Mol. Res. 12(2):
2013.January.4.4
DOI:
10.4238/2013.January.4.4
Microbial Genetics   Research Article

Highly specific detection of genetic modification events using an enzyme-linked probe hybridization chip

Authors: M.Z. Zhang, X.F. Zhang, X.M. Chen, X. Chen, S. Wu and L.L. Xu

The enzyme-linked probe hybridization chip utilizes a method based on ligase-hybridizing probe chip technology, with the principle of using thio-primers for protection against enzyme digestion, and using lambda DNA exonuclease to cut multiple PCR products obtained from the sample being tested into single-strand chains for hybridization. The 5'-end amino-l.. Read More»

Genet. Mol. Res. 14(3):
2015.August.10.10
DOI:
10.4238/2015.August.10.10
Microbial Genetics   Research Article

Estrogen receptor 1 PvuII and XbaI polymorphisms and susceptibility to Alzheimer�s disease: a meta-analysis

Authors: Y.H. Lee and G.G. Song

The aim of this study was to explore whether estrogen receptor 1 (ESR1) PvuII and XbaI polymorphisms are associated with susceptibility to Alzheimer’s disease (AD). We conducted a meta-analysis of the associations between AD and ESR1 PvuII and XbaI polymorphisms as well as haplotypes of the ESR1 PvuII and XbaI polymorphisms. A total of 1359 patients.. Read More»

Genet. Mol. Res. 14(3):
2015.August.10.17
DOI:
10.4238/2015.August.10.17
Microbial Genetics   Research Article

Protective effect of penehyclidine hydrochloride on lipopolysaccharide-induced acute kidney injury in rat

Authors: H.J. Cao, D.M. Yu, T.Z. Zhang, J. Zhou, K.Y. Chen, J. Ge and L. Pei

We aimed to observe the effect of penehyclidine hydrochloride (PHC) on lipopolysaccharide (LPS)-induced acute kidney injury in rats and expression of tight junction proteins ZO-1 and occludin. Adult male Sprague-Dawley (SD) rats were divided randomly (N = 10) into control group (C), LPS group (LPS), low-dose PHC group (L-PHC), and high-dose PHC group (H-P.. Read More»

Genet. Mol. Res. 14(3):
2015.August.10.14
DOI:
10.4238/2015.August.10.14
Microbial Genetics   Research Article

Ectopic expression of the BoTFL1-like gene of Bambusa oldhamii delays blossoming in Arabidopsis thaliana and rescues the tfl1 mutant phenotype

Authors: H.Y. Zeng, Y.T. Lu, X.M. Yang, Y.H. Xu and X.C. Lin

TERMINAL FLOWER1 (TFL1) homologous genes play major roles in maintaining vegetative growth and inflorescence meristem characteristics in various plant species; however, to date, the function of the bamboo TFL1 homologous gene has not been described. In this study, a TFL1 homologous gene was isolated from Bambusa oldhamii and designated as BoTFL1-like. Phy.. Read More»

Genet. Mol. Res. 14(3):
2015.August.10.11
DOI:
10.4238/2015.August.10.11
Microbial Genetics   Research Article

Effect of phosphatidylserine on memory in patients and rats with Alzheimer�s disease

Authors: Y.Y. Zhang, L.Q. Yang and L.M. Guo

The aim of this study was to investigate the effect of phosphatidylserine (PS) on memory of patients and rats with Alzheimer’s disease (AD). In total, 57 AD patients were recruited from our hospital, and were divided into two groups: 25 in the control group and 32 in the observation group. Next, 300 mg/d of PS was given to the rats in the observatio.. Read More»

Genet. Mol. Res. 14(3):
2015.August.10.13
DOI:
10.4238/2015.August.10.13
Microbial Genetics   Research Article

Effect of baculovirus P35 protein on apoptosis in brain tissue of rats with acute cerebral infarction

Authors: J.F. Ji and X.H. Ma

We explored the effect of baculovirus P35 protein on apoptosis in the brain tissue of rats with acute cerebral infarction (ACI). A rat model of middle cerebral artery infarction was created. The rats were randomly divided into sham, model, and treatment groups. Baculovirus P35 protein was injected into the intracranial arteries of the treatment group rats.. Read More»

Genet. Mol. Res. 14(3):
2015.August.10.16
DOI:
10.4238/2015.August.10.16
Microbial Genetics   Research Article

Aspergillus fumigatus diffusates suppress polymorphonuclear neutrophil phagocytic functions and respiratory burst levels in hematopoietic stem cell transplantation patients

Authors: X.H. Chen, Y.C. Deng, B.Y. Zhong and F. Hao

Invasive aspergillosis (IA) is a severe infection that commonly occurs in immunocompromised patients after hematopoietic stem cell transplantation (HSCT). The present study explores the effect of Aspergillus fumigatus diffusates (AfDs) on phagocytic function and superoxide anion (O2−) burst levels in polymorphonuclear neutrophils (PMNs) from post-HS.. Read More»

Genet. Mol. Res. 14(3):
2015.August.10.3
DOI:
10.4238/2015.August.10.3
Microbial Genetics   Research Article

Allelic polymorphism, gene duplication and balancing selection of the MHC class II DAB gene of Cynoglossus semilaevis (Cynoglossidae)

Authors: T.J. Xu*, Y.N. Sun* and R.X. Wang

Major histocompatibility complex (MHC) genes play an important role in the immune response of vertebrates. Allelic polymorphism and evolutionary mechanism of MHC genes have been investigated in many mammals, but much less is known in teleosts. We examined the polymorphism, gene duplication and balancing selection of the MHC cl.. Read More»

Genet. Mol. Res. 10(1):
vol10-1gmr960
DOI:
10.4238/vol10-1gmr960