Microbial Genetics
Composition and structure of microbial communities associated with different domestic sewage outfalls
Authors: Z.H. Wang, J.Q. Yang, D.J. Zhang, J. Zhou, C.D. Zhang, X.R. Su and T.W. Li
The diversity of microbiota in waste waters has not been thoroughly examined, despite the potential impact of microbes on effluent quality. Wastewater microbial communities harbor pathogenic bacteria, viruses, and parasites. To study microbial communities in domestic sewage outfalls, 454 pyrosequencing technology was used to i.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.September.12.21
- DOI:
- 10.4238/2014.September.12.21
Dynamic changes of virus load in supernatant of primary CEK cell culture infected with different generations of avian infectious bronchitis virus strains Sczy3 as revealed by real-time reverse transcription-polymerase chain reaction
Authors: Z.J. Wei, F.Y. Wang, M.P. Guo, Z.Z. Duan, N.L. Zou, P. Liu, Q.G. Yan, X.T. Wen, S.J. Cao and Y. Huang
Infectious bronchitis virus (IBV) can multiply effectively in chick embryo kidney (CEK) cells after adapting to the chick embryo. To investigate the dynamic changes in IBV load in the supernatant of primary CEK cells, we developed an SYBR Green I-based real-time polymerase chain reaction assay to quantify nucleic copy numbers of the IBV-Sczy3 strain. The .. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.11.9
- DOI:
- 10.4238/2015.June.11.9
A replicating plasmid-based vector for GFP expression in Mycoplasma hyopneumoniae
Authors: H.Z.A. Ishag, M.J. Liu, R.S. Yang, Q.Y. Xiong, Z.X. Feng and G.Q. Shao
Mycoplasma hyopneumoniae (M. hyopneumoniae) causes porcine enzootic pneumonia (PEP) that significantly affects the pig industry worldwide. Despite the availability of the whole genome sequence, studies on the pathogenesis of this organism have been limited due to the lack of a genetic manipulation system. Therefore, the aim of.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027832
- DOI:
- 10.4238/gmr.15027832
FlgN plays important roles in the adhesion of Aeromonas hydrophila to host mucus
Authors: X. Jiang, Y.X. Qin, G.F. Lin, L. Huang, B. Huang, W.S. Huangand Q.P. Yan
Adhesion to the host mucus is a crucial step in the early infection stage of pathogenic bacteria. To investigate the mechanisms of the adhesion of Aeromonas hydrophila to its host mucus, a mutant library was constructed using the mini-Tn10 transposon mutagenesis system. Of 276 individual colonies, the mutant strain with the most attenuated adhesion abilit.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.11.13
- DOI:
- 10.4238/2015.June.11.13
Characterization of Aspergillus niger ndo-1,4-�²-glucanase ENG1 secreted from Saccharomyces cerevisiae using different expression vectors
Authors: S.M. Taipakova, I.T. Smekenov, M.K. Saparbaev and A.K. Bissenbaev
Heterologous expression of Aspergillus niger endo-1,4- β-glucanase (ENG1) in Saccharomyces cerevisiae was tested both with an episomal plasmid vector (YEGAp/eng1) and a yeast vector capable of integration into the HO locus of the S. cerevisiae chromosome (pHOGAPDH-eng1-KanMX4-HO). In both cases, eng1 gene expression in yeast, with its native signal s.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.11.20
- DOI:
- 10.4238/2015.June.11.20
Genetic variability in isolates of Chromobacterium violaceum from pulmonary secretion, water, and soil
Authors: A.C. Santini, J.T. Magalh�£es, J.C.M. Cascardoâ� and R.X. Corr�ªa
Chromobacterium violaceum is a free-living Gram-negative bacillus usually found in the water and soil in tropical regions, which causes infections in humans. Chromobacteriosis is characterized by rapid dissemination and high mortality. The aim of this study was to detect the genetic variability among C. violaceum type strain A.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027955
- DOI:
- 10.4238/gmr.15027955
Microsatellite isolation and characterization for Colletotrichum spp, causal agent of anthracnose in Andean blackberry
Authors: M.L. Marulanda, A.M. L�³pez, L. Isaza and P. L�³pez
The genus Colletotrichum, comprised of pathogenic fungi that affect plants grown worldwide, causes the disease known as anthracnose in several fruit and vegetable species. Several studies conducted on plants have shown that the disease is characterized by the presence of one or several species of the fungus attacking .. Read More»
- Genet. Mol. Res. 13(3):
- 2014.September.26.5
- DOI:
- 10.4238/2014.September.26.5
Genetic diversity of Mycosphaerella fijiensis in Brazil analyzed using an ERIC-PCR marker
Authors: G.F. Silva, R.D.V. Paix�£o, C.B. Queiroz, M.F. Santana, A. Souza, N.R. Sousa, R.E. Hanada and L. Gasparotto
The Enterobacterial repetitive intergenic consensus (ERIC) marker was used to analyze the genetic variability of Mycosphaerella fijiensis, the causative agent of Black Sigatoka disease in banana plants. A total of 123 isolates were used, which were divided into populations based on their original hosts and collection .. Read More»
- Genet. Mol. Res. 13(3):
- 2014.September.26.7
- DOI:
- 10.4238/2014.September.26.7
Hemerythrin is required for Aeromonas hydraphlia to survive in the macrophages of Anguilla japonica
Authors: W.B. Zeng, W.B. Chen, Q.P. Yan, G.F. Lin and Y.X. Qin
Survival in host phagocytes is an effective strategy for pathogenic microbes to spread. To understand the mechanisms of Aeromonas hydrophila survival within host macrophages, a library of mini-Tn10 transposon insertion mutants was constructed. The M85 mutant, whose survival in host macrophages was only 23.1% of that of the wil.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028074
- DOI:
- 10.4238/gmr.15028074
Experimental strategies in carrying out VCU for tobacco crop I: plot design and size
Authors: F.H.R.B. Toledo, M.A.P. Ramalho, C.E. Pulcinelli and A.T. Bruzi
We aimed to establish standards for tobacco Valor de Cultivo e Uso (VCU) in Brazil. We obtained information regarding the size and design of plots of two varietal groups of tobacco (Virginia and Burley). Ten inbred lines of each varietal group were evaluated in a randomized complete block design with four replications. The plot contained 42 plants with si.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.19.8
- DOI:
- 10.4238/2013.September.19.8