Microbial Genetics
Identification of actinomycete communities in Antarctic soil from Barrientos Island using PCR-denaturing gradient gel electrophoresis
Authors: L. Learn-Han, C. Yoke-Kqueen, M.S. Shiran, C.M.W. Vui-Ling, A.M. Nurul-Syakima, R. Son and H.M. Andrade
The diversity of specific bacteria taxa, such as the actinomycetes, has not been reported from the Antarctic island of Barrientos. The diversity of actinomycetes was estimated with two different strategies that use PCR-denaturing gradient gel electrophoresis. First, a PCR was applied, using a group-specific primer that allows .. Read More»
- Genet. Mol. Res. 11(1):
- http://dx.doi.org/2012.February.8.3
- DOI:
- http://dx.doi.org/10.4238/2012.February.8.3
Cloning, expression, and characterization of Fe-SOD from Is�¶etes sinensis
Authors: Y. Xu, X.L. Dai, B.D. Liu and Q.X. Wang
Although the palynology and sporophyte stage of Isöetes sinensis have been well studied, the biology of its gametophyte and embryo is less well understood. To date, the functions of several genes of I. sinensis and the molecular mechanisms of enzymes encoded by them remain to be studied. In the present study, the Fe-SOD g.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15047131
- DOI:
- 10.4238/gmr15047131
Molecular cloning of HSP70 in Mycoplasma ovipneumoniae and comparison with that of other mycoplasmas
Authors: M. Li, C.J. Ma, X.M. Liu, D. Zhao, Q.C. Xu and Y.J. Wang
Mycoplasma ovipneumoniae, a bacterial species that specifically affects ovine and goat, is the cause of ovine infectious pleuropneumonia. We cloned, sequenced and analyzed heat shock protein 70 (HSP70) (dnaK) gene of M. ovipneumoniae. The full length open reading frame of the M. ovipneumoniae HSP70 gene consists of 1812 nucleo.. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr1193
- DOI:
- 10.4238/vol10-2gmr1193
Parathyroid hormone-related peptide (PTHrP): prokaryotic expression, purification, and preparation of a polyclonal antibody
Authors: H.L. Zheng, H. Li, Y.S. Sun, Z.Y. Yang and Q. Yu
Parathyroid hormone-related peptide (PTHrP) plays important roles in promoting cancer occurrence and in the development of bone metastases. To increase our knowledge of the biological functions of PTHrP, the prokaryotic expression vector pET-PTHrP was successfully constructed and the His-PTHrP fusion protein was expressed in .. Read More»
- Genet. Mol. Res. 13(3):
- 2014.August.25.8
- DOI:
- 10.4238/2014.August.25.8
Significance of the expression of integrin �²1, VEGF and MVD in hypopharyngeal squamous cell carcinoma
Authors: Y.M. Hong, W.G. Gan and Z.H. Xu
This study aimed to determine the expression of integrin β1 and vascular endothelial growth factor (VEGF) and microvascular density (MVD) by CD105 staining in hypopharyngeal squamous cell carcinoma to determine their association with clinicopathologic characteristics, and to determine their role and the effects of their i.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.August.25.9
- DOI:
- 10.4238/2014.August.25.9
A new high-frequency allele of the BM2113 locus in the Yunnan mithun population
Authors: K.-X. Qu, Z.-X. He, R.-J. Hao, J.-C. Zhang, B.-Z. Huang,L.-S. Zan, and Y.-P. Zhang
The BM2113 locus was amplified in Yunnan mithun (Bos frontalis) from the southwest mountains of China. It showed a high degree of polymorphism with a total of 12 alleles. The 121-bp polymorphic allele of the BM2113 locus that accounted for 37.1% of homozygotes was the predominant allele with a frequency of 58.57%, identified a.. Read More»
- Genet. Mol. Res. 13(1):
- 2014.March.24.23
- DOI:
- 10.4238/2014.March.24.23
Highly efficient one-step PCR-based mutagenesis technique for large plasmids using high-fidelity DNA polymerase
Authors: H. Liu, R. Ye and Y.Y. Wang
Gene mutation plays an important role in molecular biological studies. A highly efficient one-step polymerase chain reaction-based mutagenesis technique for site-directed mutagenesis was developed in this study. One complementary pair of primers was designed that contained the desired mutations in the middle of the primers. The amplification products of m.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.15.10
- DOI:
- http://dx.doi.org/10.4238/2015.April.15.10
Potassium contributes to zinc stress tolerance in peach (Prunus persica) seedlings by enhancing photosynthesis and the antioxidant defense system
Authors: Z.Z. Song, C.L. Duan, S.L. Guo, Y. Yang, Y.F. Feng, R.J. Ma and M.L. Yu
Zinc (Zn) is considered to be a major industrial pollutant because excessive amounts can impair plant growth. In this paper, we found that peach ‘Yoshihime’ seedlings are promising Zn tolerant plants. However, heavy Zn toxicity (2 mM) damaged plant performance by disrupting biochemical processes, including photosynthesis, proline production, a.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.27.22
- DOI:
- 10.4238/2015.July.27.22
Relationship between urinary protein changes in lupus nephritis and renal pathology
Authors: M. Li, W.J. Gao, J.J. Ma, Y. Zhu and X.F. Li
This study investigated the relationship between urinary protein excretion in lupus nephritis New Zealand black mice and renal pathology. A total of 328 lupus nephritis New Zealand black mice were established by a backcross hybridization method, and renal pathology was determined. The urinary protein excretion of the backcross mice over 24 h was compared .. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.28.1
- DOI:
- 10.4238/2015.July.28.1
Regulators of G-protein signaling 9 genetic variations in Chinese subjects with schizophrenia
Authors: Y.S. Zhu, Y.X. Li, X.M. Qiao and H.B. Zhang
To identify single-nucleotide polymorphisms that contribute to the genetic susceptibility to schizophrenia, we examined the potential association between schizophrenia and 9 single nucleotide polymorphisms (rs1530351, rs4791230, rs2869577, rs8077696, rs8070231, rs2292592, rs9916525, rs1122079, and rs4790953) in the G-protein signaling 9 gene. The particip.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.28.13
- DOI:
- 10.4238/2015.July.28.13