Microbial Genetics
Outbreaks and genetic diversity of Francisella noatunensis subsp orientalis isolated from farm-raised Nile tilapia (Oreochromis niloticus) in Brazil
Authors: C.A.G. Leal, G.C. Tavares and H.C.P. Figueiredo
Francisella noatunensis subsp orientalis (FNO) is an emerging pathogen of warm water tilapia in a number of different countries. The disease caused by this bacterium in fish is characterized by a systemic granulomatous infection that causes high mortality rates during outbreaks. FNO has been previously described in As.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.25.26
- DOI:
- 10.4238/2014.July.25.26
Alteration of coenzyme specificity of malate dehydrogenase from Streptomyces coelicolor A3(2) by site-directed mutagenesis
Authors: Y.D. Ge, P. Song, Z.Y. Cao, P. Wang and G.P. Zhu
We describe here for the first time the alteration of coenzyme specificity of malate dehydrogenase (MDH) from Streptomyces coelicolor A3(2) (ScMDH). In the present study, we replaced four amino acid residues in the Rossmann fold (βB-αC) region of NADH-dependent ScMDH by site-directed mutagenesis with those of NADPH-dependent MDH (Glu42.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.29.3
- DOI:
- 10.4238/2014.July.29.3
In vitro antioxidant activities of endophytic fungi isolated from the liverwort Scapania verrucosa
Authors: P.Y. Zeng, J.G. Wu, L.M. Liao, T.-Q. Chen, J.Z. Wu and K.-H. Wong
We investigated in vitro antioxidant activities of 49 endophytic fungi isolated from the liverwort Scapania verrucosa. Based on morphological and molecular identification, the endophytic fungi isolated were classified into seven genera (Hypocrea, Penicillium, Tolypocladium, Chaetomium, Xylaria, Nemania, and Creosphaeria), all .. Read More»
- Genet. Mol. Res. 10(4):
- 2011.December.20.1
- DOI:
- 10.4238/2011.December.20.1
Isolation and characterization of yeasts capable of efficient utilization of hemicellulosic hydrolyzate as the carbon source
Authors: L.A. Cassa-Barbosa, R.E.L. Proc�³pio, I.T.S.R. Matos and S.A. Filho
Few yeasts have shown the potential to efficiently utilize hemicellulosic hydrolyzate as the carbon source. In this study, microorganisms isolated from the Manaus region in Amazonas, Brazil, were characterized based on their utilization of the pentoses, xylose, and arabinose. The yeasts that showed a potential to assimilate these sugars were selected for .. Read More»
- Genet. Mol. Res. 14(3):
- http://dx.doi.org/2015.September.28.12
- DOI:
- http://dx.doi.org/10.4238/2015.September.28.12
Sequence analysis of the PIP5K locus in Eimeria maxima provides further evidence for eimerian genome plasticity and segmental organization
Authors: B.K. Song, M.Z. Pan, Y.L. Lau and K.L. Wan
Commercial flocks infected by Eimeria species parasites, including Eimeria maxima, have an increased risk of developing clinical or subclinical coccidiosis; an intestinal enteritis associated with increased mortality rates in poultry. Currently, infection control is largely based on chemotherapy or live vacci.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.29.8
- DOI:
- 10.4238/2014.July.29.8
Effects of 6-week specific low-intensity training on selected aerobic capacity parameters and HSPA1A, HSPB1, and LDHb gene expression in high-level rowers
Authors: Z. Jastrz��bski and M. �»ychowska
The aim of this study was to demonstrate the effects of 6-week low-intensity training on changes in indicators of aerobic capacity and on HSPA1A, HSPB1, and LDHb expression in white blood cells in high level rowers. We hypothesized that the type of training would have an impact not only on the adaptation of athletes to the aerobic nature of the exercises,.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.3.29
- DOI:
- 10.4238/2015.July.3.29
Analysis of DNA polymerase activity in vitro using non-radioactive primer extension assay in an automated DNA sequencer
Authors: Lopes DO, Regis-da-Silva CG, Machado-Silva A, Macedo AM, Franco GR, Hoffmann JS, Cazaux C, Pena SDJ, Teixeira SMR and Machado CR
Although different DNA polymerases have distinct functions and substrate affinities, their general mechanism of action is similar. Thus, they can all be studied using the same technical principle, the primer extension assay employing radioactive tags. Even though fluorescence has been used routinely for many years for DNA sequ.. Read More»
- Genet. Mol. Res. 6(2):
Endophytic bacteria from Piper tuberculatum Jacq.: isolation, molecular characterization, and in vitro screening for the control of Fusarium solani f. sp piperis, the causal agent of root rot disease in black pepper (Piper nigrum L.)
Authors: S.B. Nascimento, A.M. Lima, B.N. Borges and C.R.B. de Souza
Endophytic bacteria have been found to colonize internal tissues in many different plants, where they can have several beneficial effects, including defense against pathogens. In this study, we aimed to identify endophytic bacteria associated with roots of the tropical piperaceae Piper tuberculatum, which is known for itsresistance to infection by Fusariu.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.3.32
- DOI:
- 10.4238/2015.July.3.32
Synergistic effect of BMP9 and TGF-�² in the proliferation and differentiation of osteoblasts
Authors: X.L. Li, Y.B. Liu, E.G. Ma, W.X. Shen, H. Li and Y.N. Zhang
We investigated the synergistic effect of bone morphogenetic protein 9 (BMP9) and transforming growth factor (TGF)-b in the transformation of mesenchymal stem cells into osteoblasts. We evaluated the effect of BMP9 and TGF-b on the induction of osteoblast formation. Mitogen-activated protein kinase (MAPK) pathway-related proteins such as p38, extracellula.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.13.4
- DOI:
- 10.4238/2015.July.13.4
Isolation and characterization of microsatellite loci in the purpleback flying squid (Sthenoteuthis oualaniensis)
Authors: L. Lin, P. Li, Z.Z. Chen, Y.Y. Xiao, S.N. Xu, Y. Liu and C.H. Li
The purpleback flying squid (Sthenoteuthis oualaniensis) is a pelagic squid with tremendous potential for commercial exploitation. We isolated and characterized 21 polymorphic microsatellite loci for S. oualaniensis using a (GT)13-enriched genomic library. The number of alleles per locus varied from 6 to 32. The observed and expected heterozygosities rang.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.13.7
- DOI:
- 10.4238/2015.July.13.7