Microbial Genetics

DNA sequencing of a pathogenicity-related plasmid of an avian septicemic Escherichia coli strain

Authors: Stehling EG, Campos TA, Azevedo V, Brocchi M and Silveira WD

A 43-MDa conjugative plasmid isolated from an avian septicemic Escherichia coli (APEC) strain possessing genes related to the adhesion and invasion capacities of in vitro-cultured cells was sequenced. The results demonstrated that the 43-MDa plasmid harbors bacterial pathogenicity-related sequences which probably allow the wil.. Read More»

Genet. Mol. Res. 6(2):

Isolation and characterization of polymorphic microsatellite loci for Pachycrepoideus vindemmiae (Rondani) (Hymenoptera: Pteromalidae)

Authors: W. Chen, L. Fang, J.L. Liu, Z. He and H.Y. Hu

The parasitoid wasp Pachycrepoideus vindemmiae (Rondani) is a common pupal parasitoid of many fly pests that is distributed worldwide. This organism can be used for biological control in orchards or livestock farms. Identifying polymorphic microsatellite loci would be useful for analyzing the population genetic structure of the parasitoid. In the current .. Read More»

Genet. Mol. Res. 14(1):

2015.March.13.7

DOI:

10.4238/2015.March.13.7

Identification of aac(2')-I Type b aminoglycoside-modifying enzyme genes in resistant Acinetobacter baumannii

Authors: T. Lin, C.G. Tang, Q.H. Li, J. Ji, H.Y. Ge, X.Y. Zhang and H.P. Sun

The aim of this study was to investigate the mechanism underlying the drug resistance of Acinetobacter baumannii toward aminoglycosides. A total of 32 A. baumannii strains were identified by molecular identification and subsequently isolated. The isolates were then amplified by polymerase chain reaction to analyze the 9 aminoglycoside-modifying enzyme gen.. Read More»

Genet. Mol. Res. 14(1):

2015.March.13.11

DOI:

10.4238/2015.March.13.11

Screening of rice (Oryza sativa L.) OsPR1b-interacting factors and their roles in resisting bacterial blight

Authors: Z.H. Luan and D.W. Zhou

PR genes, a type of genetic marker, are constitutively expressed at background levels, while being easily inducible by pathogenic bacteria. By using a yeast two-hybrid technique, four rice (Oryza sativa L.) OsPR1b-interacting factors were screened. Homozygous plants overexpressing OsPR1b were prepared by transgenic technology. We postulated that OsPR1b ma.. Read More»

Genet. Mol. Res. 14(1):

2015.March.13.15

DOI:

10.4238/2015.March.13.15

Comparison of simple sequence repeats in 19 Archaea

Authors: S. Trivedi

All organisms that have been studied until now have been found to have differential distribution of simple sequence repeats (SSRs), with more SSRs in intergenic than in coding sequences. SSR distribution was investigated in Archaea genomes where complete chromosome sequences of 19 Archaea were analyzed with the program SPUTNIK.. Read More»

Genet. Mol. Res. 5(4):

Assessment of genetic diversity and relationships among wild and cultivated Tunisian plums (Prunus spp) using random amplified microsatellite polymorphism markers

Authors: H. Ben Tamarzizt, S. Ben Mustapha, G. Baraket, D. Abdallah and A. Salhi-Hannachi

Genet. Mol. Res. 14(1):

2015.March.20.4

DOI:

10.4238/2015.March.20.4

Association between the hsa-miR-146a rs2910164 functional polymorphism with susceptibility to intracranial aneurysm

Authors: X.T. Sima, J.G. Xu, J. Li and C. You

Vascular inflammation has been shown to be involved in the pathogenesis of intracranial aneurysms (IA). MiRNAs are key molecules that participate in the regulation of many important biological processes including inflammation. Studies on the hsa-miR-146a rs2910164 polymorphism and its association with different inflammatory related diseases have engendere.. Read More»

Genet. Mol. Res. 14(3):

2015.July.13.13

DOI:

10.4238/2015.July.13.13

Genetic diversity of the endemic flat-needle pine Pinus krempfii (Pinaceae) from Vietnam revealed by SSR markers

Authors: D.T. Phong, T.T. Lieu, V.T.T. Hien and N.T. Hiep

Pinus krempfii Lecomte (Pinaceae) is an endemic tree to Vietnam with restricted habitats at higher altitudes in the highlands. In this study, genetic variation of four populations of P. krempfii was assessed using 17 microsatellite markers (single sequence repeats). Of these 17 markers, eight were polymorphic, and among the 42 putative alleles amplified, .. Read More»

Genet. Mol. Res. 14(3):

2015.July.13.19

DOI:

10.4238/2015.July.13.19

Construction and partial characterization of a Corynebacterium pseudotuberculosis bacterial artificial chromosome library through genomic survey sequencing

Authors: F.A. Dorella, M.S. Fachin, A. Billault, E. Dias Neto, C. Soravito, S.C. Oliveira, R. Meyer, A. Miyoshi and V. Azevedo

Corynebacterium pseudotuberculosis is a gram-positive bacterium that causes caseous lymphadenitis in sheep and goats. However, despite the economic losses caused by caseous lymphadenitis, there is little information about the molecular mechanisms of pathogenesis of this bacterium. Genomic libraries constructed in bacterial art.. Read More»

Genet. Mol. Res. 5(4):

Polymorphic microsatellite loci developed from the Hong Kong oyster (Crassostrea hongkongensis)

Authors: H.T. Ma, S. Xiao, Y. Zhang, X.M. Li, J. Li and Z.N. Yu

Forty polymorphic microsatellite loci were developed from Crassostrea hongkongensis using an enriched partial genomic library with magnetic beads. The polymorphism of these loci was assessed in 30 individuals from a wild population. The allele number of the polymorphic markers ranged from 2 to 13, with an average of 5.8 per lo.. Read More»

Genet. Mol. Res. 15(4):

gmr.15048676

DOI:

10.4238/gmr.15048676