Microbial Genetics
Effects of phosphate-solubilizing bacteria, native microorganisms, and rock dust on Jatropha curcas L. growth
Authors: E.B. Santana, E.L.S. Marques and J.C.T. Dias
Microorganisms with the ability to release nutrients to the soil from insoluble sources may be useful for plant cultivation. We evaluated the growth-promoting effect on Jatropha curcas L. of phosphate-solubilizing bacteria (PSB) and the native microbiota in soil with or without rock dust. J. curcas L. is important for biodiese.. Read More»
- Genet. Mol. Res. 15(4):
- gmr.15048729
- DOI:
- 10.4238/gmr.15048729
Rapid and efficient protocol for DNA extraction and molecular identification of the basidiomycete Crinipellis perniciosa
Authors: S.C.O. Melo, C. Pungartnik, J.C.M. Cascardo and M. Brendel
DNA isolation from some fungal organisms is difficult because they have cell walls or capsules that are relatively unsusceptible to lysis. Beginning with a yeast Saccharomyces cerevisiae genomic DNA isolation method, we developed a 30-min DNA isolation protocol for filamentous fungi by combining cell wall digestion with cell d.. Read More»
- Genet. Mol. Res. 5(4):
The use of the acridine orange test and the TUNEL assay to assess the integrity of freeze-dried bovine spermatozoa DNA
Authors: Martins CF, Dode MN, Bao SN and Rumpf R
The ability to detect nuclear damage is an important tool for the development of sperm preservation methods. We used the acridine orange test (AOT) and the terminal deoxynucleotidyl transferasemediated dUDP nick-end labeling (TUNEL) assay to assess the DNA status of sperm cells preserved with different lyophilization media. Th.. Read More»
- Genet. Mol. Res. 6(1):
Branches of NF-�ºb signaling pathway regulate hepatocyte proliferation in rat liver regeneration
Authors: C.F. Chang, W.M. Zhao, J.X. Mei, Y. Zhou, C.Y. Pan, T.T. Xu and C.S. Xu
Previous studies have demonstrated that the nuclear factor κB (NF-κB) pathway is involved in promoting cell proliferation. To further explore the regulatory branches and their sequence in the NF-κB pathway in the promotion of hepatocyte proliferation at the transcriptional level during rat liver regeneration, Rat Genome 230 2.0 array was.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.13.9
- DOI:
- 10.4238/2015.July.13.9
Evaluation of polyvinyl alcohol for fatty acid supplementation in adipose tissue explant culture
Authors: Jose AAFBV, Gama MAS, Urban A, Merighe GK, Meirelles FV, Etchegaray MAL and Lanna DPD
Cultures of adipose tissue explants are a valuable tool for studying the intracellular mechanisms involving hormones and nutrients. However, testing how fatty acids affect cells requires a carrier molecule; bovine serum albumin (BSA) has been used for this purpose. However, contaminants can alter the cellular response. Our obj.. Read More»
- Genet. Mol. Res. 6(1):
Fructus polygoni orentalis extract inhibited liver regeneration and proliferation of bone marrow cells of rat after partial hepatectomy
Authors: J.Y. Yang, J.S. Wang and H.B. Liu
To study the effect of fructus polygoni orentalis extract (EFPO) on liver regeneration and proliferation of bone marrow cells on rat model of partial hepatectomy, EFPO was extracted, and 60 adult male Wistar rats were divided randomly into 6 experimental groups. Rats were treated with intergastric administration (ig) with EFPO daily. All rats were euthani.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.13.12
- DOI:
- 10.4238/2015.July.13.12
Age-related pyrethroid resistance is not a function of P450 gene expression in the major African malaria vector, Anopheles funestus (Diptera: Culicidae)
Authors: R.N. Christian, T.S. Matambo, B.L. Spillings, B.D. Brooke, M. Coetzee and L.L. Koekemoer
Anopheles funestus is a major vector of malaria in most of the African region. Resistance to pyrethroid and carbamate insecticides has been recorded in populations of this species in South Africa and Mozambique. The P450 gene, CYP6P9, has been shown to be highly transcribed in a permethrin (pyrethroid)-resistant laboratory str.. Read More»
- Genet. Mol. Res. 10(4):
- /2011.December.21.4
- DOI:
- /10.4238/2011.December.21.4
Lapachol as an epithelial tumor inhibitor agent in Drosophila melanogaster heterozygote for tumor suppressor gene wts
Authors: W.F. Costa, A.B. Oliveira and J.C. Nepomuceno
The search for new and effective antitumor agents with fewer cytotoxic side effects on normal tissue has increasingly become important. Lapachol, a natural organic compound isolated from the lapacho tree (Tabebuia avellandedae), is chemically identified as belonging to the naphthoquinone group and is known for its anti-inflamm.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.December.22.1
- DOI:
- 10.4238/2011.December.22.1
Stimulation of bacterial biofilms on Th17 immune cells
Authors: G.Q. Wang, L. Wang, H.L. Zhang, Y.Q. Dong and Y.X. Yang
We investigated the role of bacterial biofilms in stimulating T helper 17 (Th17) cells in infected organisms. The formation of bacterial biofilms isolated from clinical lavage fluid samples was measured. Th17 cells and interleukin 17 (IL-17) levels in the peripheral blood of healthy individuals, people infected by biofilm bacteria, people infected by non-.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.13.18
- DOI:
- 10.4238/2015.July.13.18
Identification of non-specific alkaline phosphatases in hyphal cells of the fungus Neurospora crassa by in situ histochemistry
Authors: G. Thedei Junior and A. Rossi
The present study was designed to identify alkaline phosphatases in non-permeabilized hyphal cells of the fungus Neurospora crassa by staining these enzymatic activities with a modified azo dye coupling method. Our strategy allowed the identification of three nonspecific alkaline phosphatase activities, one of them possibly be.. Read More»
- Genet. Mol. Res. 5(3):