Microbial Genetics
Genetic fidelity and variability of micropropagated cassava plants (Manihot esculenta Crantz) evaluated using ISSR markers
Authors: ?.M. Vidal L.J. Vieira, C.F. Ferreira, F.V.D. Souza, A.S. Souza and C.A.S. Ledo
Molecular markers are efficient for assessing the genetic fidelity of various species of plants after in vitro culture. In this study, we evaluated the genetic fidelity and variability of micropropagated cassava plants (Manihot esculenta Crantz) using inter-simple sequence repeat markers. Twenty-two cassava accessions from the Embrapa Cassava & Fruits.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.14.2
- DOI:
- 10.4238/2015.July.14.2
Manipulation of primer affinity improves high-resolution melting accuracy for imprinted genes
Authors: F.V.M. Rubatino, N.V. Carobin, M.L. Freitas, V.T. de Oliveira, R.X. Pietra, P.P.R. Oliveira, A.A. Bosco and F.S. Jehee
High-resolution melting (HRM) is considered an inexpensive, rapid, and attractive methodology for methylation analysis. In the application of the polymerase chain reaction (PCR) to methylation analysis, amplification efficiencies are biased towards unmethylated, rather than methylated, templates: a phenomenon known as PCR bias. To overcome PCR bias, prime.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.14.12
- DOI:
- 10.4238/2015.July.14.12
Development of EST-SSR and TRAP markers from transcriptome sequencing data of the mango
Authors: C. Luo, H.X. Wu, Q.S. Yao, S.B. Wang and W.T. Xu
Mango is one of the most commercially important fruit crops in tropical and subtropical regions. To increase the efficiency of breeding strategies, two EST-derived marker systems were developed in the present study using information from the mango fruit transcriptome. Using simple sequence repeats, 218 of 230 primer pairs showed stable amplification for 7.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.14.17
- DOI:
- 10.4238/2015.July.14.17
Association between the rs3795879 G/A polymorphism of the SERPINE2 gene and chronic obstructive pulmonary disease: a meta-analysis
Authors: D.B. Tian, S.X. Cai, G.C. Pan, Y.F. Zhang and J.H. Xiao
The present study aimed to investigate the effects of the rs3795879 polymorphism of the SERPINE2 gene on the development of chronic obstructive pulmonary disease (COPD) based on a systematic meta-analysis. An extensive literature search was performed to retrieve previously published case-control studies on the polymorphisms of SERPINE2 in COPD patients. O.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.14.18
- DOI:
- 10.4238/2015.July.14.18
Evaluation of a modified sampling method for molecular analysis of air microflora
Authors: T. Lech and A. Ziembinska-Buczynska
A serious issue concerning the durability of economically important materials for humans related to cultural heritage is the process of biodeterioration. As a result of this phenomenon, priceless works of art, documents, and old prints have undergone a process of decomposition caused by microorganisms. Therefore, it is important to constantly monitor the .. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.10.32
- DOI:
- http://dx.doi.org/10.4238/2015.April.10.32
Source of bacterial RNA in chronic otitis media with effusion
Authors: P.-Z. Li, L. Cheng and M.-L. Qiu
The purpose of this study was to investigate whether the bacterial RNA detected by polymerase chain reaction (PCR) and reverse transcription (RT)-PCR methods in middle ear effusion (MEE) for pediatric chronic otitis media with effusion (OME) originated from live bacteria. Degradation of RNA was observed by spectroscopic analys.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.August.7.24
- DOI:
- 10.4238/2014.August.7.24
Regulation of expression of sodA and msrA genes of Corynebacterium glutamicum in response to oxidative and radiative stress
Authors: H.M. El Shafey and S. Ghanem
Promoters of genes encoding superoxide dismutase (sodA) and peptide methionine sulfoxide reductase (msrA) from Corynebacterium glutamicum were cloned and sequenced. Promoter region analysis of sodA-msrA was unable to identify putative sites of fixed eventual regulators except for possible sites of fixed OxyR and integration host factor. A study .. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.20.21
- DOI:
- 10.4238/2015.March.20.21
Comprehensive identification and expression analysis of Hsp90s gene family in Solanum lycopersicum
Authors: W.S. Zai, L.X. Miao, Z.L. Xiong, H.L. Zhang, Y.R. Ma, Y.L. Li, Y.B. Chen and S.G. Ye
Heat shock protein 90 (Hsp90) is a protein produced by plants in response to adverse environmental stresses. In this study, we identified and analyzed Hsp90 gene family members using a bioinformatic method based on genomic data from tomato (Solanum lycopersicum L.). The results illustrated that tomato contains at least 7 Hsp90 genes distributed on 6 chrom.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.14.7
- DOI:
- 10.4238/2015.July.14.7
Rapid development of polymorphic microsatellite markers for the Amur sturgeon (Acipenser schrenckii) using next-generation sequencing technology
Authors: L.M. Li, L. Wei, H.Y. Jiang, Y. Zhang, X.J. Zhang, L.H. Yuan and J.P. Chen
Anthropogenic activities have seriously impacted wild resources of the Amur sturgeon, Acipenser schrenckii, and more information on local and regional population genetic structure is required to aid the conservation of this species. In this study, we report the development of 12 novel polymorphic microsatellite loci using next-generation sequencing techno.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.14.16
- DOI:
- 10.4238/2015.July.14.16
Usefulness of direct sequencing of pooled DNA for SNP identification and allele-frequency determination compatible with a common disease/common variant hypothesis
Authors: M.K. Kim, T.S. Nam, K.H. Choi, S.Y. Jang, Y.O. Kim and M.C. Lee
We examined the efficiency of direct sequencing of pooled DNA for developing common single nucleotide polymorphisms (SNPs) and its accuracy for estimating allele frequencies. A pool of 200 control DNAs was established and was used for developing SNPs and estimating minor allele frequencies (MAF). The sensitivity of the pooled .. Read More»
- Genet. Mol. Res. 9(2):
- vol9-2gmr761
- DOI:
- 10.4238/vol9-2gmr761