Microbial Genetics
Propofol suppresses proliferation and invasion of gastric cancer cells via downregulation of microRNA-221 expression
Authors: Z.T. Wang, H.Y. Gong, F. Zheng, D.J. Liu and X.Q. Yue
Propofol is one of the extensively and commonly used intravenous anesthetic agents. The current study aimed to evaluate the effects of propofol on the behavior of human gastric cancer cells and the molecular mechanisms of this activity. The effects of propofol on SGC7901 and AGS cell proliferation, apoptosis, and invasionwere detected by MTT assay, flow c.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.17.20
- DOI:
- 10.4238/2015.July.17.20
Transferability of microsatellite markers of Capsicum annuum L. to C. frutescens L. and C. chinense Jacq.
Authors: S.I.C. Carvalho, C.F. Ragassi, I.B. Oliveira, Z.P.S. Amaral, F.J.B. Reifschneider, F.G. Faleiro and G.S.C. Buso
In order to support further genetic, diversity, and phylogeny studies of Capsicum species, the transferability of a Capsicum annuum L. simple sequence repeat (SSR) microsatellite set was analyzed for C. frutescens L. (“malagueta” and “tabasco” peppers) and C. chinense Jacq. (smell peppers, among other types). A total of 185 SSR pri.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.17.1
- DOI:
- 10.4238/2015.July.17.1
Relationship between UV-irradiated HaCaT cell cytokines and Th1/Th2 imbalance
Authors: H.Y. Li, F.R. Zhang and D.Q. Deng
We have previously found that an imbalance of Tc1/Tc2 T cell subtypes in vivo impacts the development of photodermatitis. The aim of this study was to investigate the relationship between cytokines derived from keratinocytes exposure to UV and the imbalance of Th subgroups. We used different doses of UVA and UVB to irradiate HaCaT cells. Twelve hours afte.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.17.5
- DOI:
- 10.4238/2015.July.17.5
Characterization of FeDREB1 promoter involved in cold- and drought-inducible expression from common buckwheat (Fagopyrum esculentum)
Authors: Z.W. Fang, X.Y. Xu, J.F. Gao, P.K. Wang, Z.X. Liu and B.L. Feng
C-repeat-binding factor (CBF)/dehydration-responsive element (DREB) transcription factors play key roles in plant stress responses. However, little information is available on the regulation of CBF/DREB expression. In this study, we isolated and characterized the FeDREB1 promoter sequence from the common buckwheat accession Xinong 9976. To identify the up.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.17.7
- DOI:
- 10.4238/2015.July.17.7
Correlation between MTHFR gene methylation and pre-eclampsia, and its clinical significance
Authors: J. Ge, J. Wang, F. Zhang, B. Diao, Z.F. Song, L.L. Shan, W. Wang, H.J. Cao and X.Q. Li
We investigated the correlation between 5,10-methylenetetrahydrofolate reductase (MTHFR) gene methylation and pre-eclampsia, and its clinical significance, by comparing methylation in the MTHFR gene promoter of the placenta and peripheral venous blood in pre-eclampsia and normal gravidas. We enrolled 259 gravidas from the People’s Liberation Army 20.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.17.10
- DOI:
- 10.4238/2015.July.17.10
Lentinan depresses 3T3-L1 fat cell formation by inhibiting PPAR�³/AKT signaling pathway
Authors: D.G. Li, J.L. Li, D.Q. Sun, X.B. Sun, X.G. Sun and Q. Liu
We investigated the mechanism of the effect of lentinan on 3T3-L1 fat cell formation by inhibiting the peroxisome proliferator-activated receptor gamma (PPARg)/protein kinase B (AKT) signaling pathway. 3T3-L1 fat cells were treated with 80 mM lentinan with or without the PPARg activator, 100 mM rosiglitazone for 24 h. Reverse transcription-polymerase chai.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.17.17
- DOI:
- 10.4238/2015.July.17.17
Selection and molecular identification of fungal isolates that produce xylanolytic enzymes
Authors: M. Alvarez-Navarrete, G.E. Reyna LÃ?³pez, A. Flores-GarcÃ?Âa, R. LÃ?³pez GÃ?³mez and M.M. MartÃ?Ânez-Pacheco
Xylan is a heteropolysaccharide and its complete hydrolysis involves a complex set of xylanolytic enzymes. Fungal xylanases have been widely used in the holocellulose industry to obtain by-products or for its elimination. The aim of this study was to select and identify filamentous fungi from different ecosystems that produce extracellular xylanases showi.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.17.19
- DOI:
- 10.4238/2015.July.17.19
Estimation of genetic structure of a Mycosphaerella musicola population using inter-simple sequence repeat markers
Authors: Y.S. Peixouto, C.A. D�³rea Bragan�§a, W.B. Andrade, C.F. Ferreira, F. Haddad, S.A.S. Oliveira, F.S. Darosci Brito, R.N.G. Miller and E.P. Amorim
Among the diseases affecting banana (Musa sp), yellow Sigatoka, caused by the fungal pathogen Mycosphaerella musicola Leach, is considered one of the most important in Brazil, causing losses throughout the year. Understanding the genetic structure of pathogen populations will provide insight into the life history of pathogens, including the evolutionary p.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.17.13
- DOI:
- 10.4238/2015.July.17.13
Isolation, partial characterization, and cloning of an extracellular chitinase from the entomopathogenic fungus Verticillium lecanii
Authors: G. Yu, L.Q. Xie, J.T. Li, X.H. Sun, H. Zhang, Q. Du, Q.Y. Li,S.H. Zhang and H.Y. Pan
The entomopathogenic fungus Verticillium lecanii is a well-known biocontrol agent of fungal phytopathogens, as well as insect pests. A 42-kDa chitinase belonging to family 18 of the glycosyl hydrolases was isolated and partially characterized. Chitinase was purified using successive column chromatography on phenyl-sepharose, DEAE-sepharose, and CM-sepharo.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.27.13
- DOI:
- 10.4238/2015.March.27.13
Molecular cloning, characterization, and expression of vacuolar-type-H+-ATPase B1 (VHAB1) gene in the gill of Anguilla marmorata
Authors: L. Li, Y.H. Jia, P. Li, S.W. Yin, G.S. Zhang, X.L. Wang, Y.Y. Wang and X.J. Wang
We explored the molecular mechanism of the regulation of vacuolar-type-H+-ATPase B1 (VHAB1) in elvers in the response to salinity. The full-length cDNA of VHAB1 in Anguilla marmorata (designated as AmVHAB1), which was 1741 base pairs (bp) in length, was found to encompass a 1512-bp open reading frame encoding a polypeptide with 503 amino acids (55.9 kDa),.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.17.9
- DOI:
- 10.4238/2015.July.17.9