Microbial Genetics
Systematic meta-analysis of the association between monocyte chemoattractant protein-1 -2518A/G polymorphism and risk of tuberculosis
Authors: G. Tian, X. Li, H. Li, X. Wang and B. Cheng
Numerous studies have been conducted to investigate the association between 2518A/G polymorphisms in the monocyte chemoattractant protein-1 (MCP-1) gene and the risk of tuberculosis (TB). However, the results have been inconsistent and inconclusive. In this study, we performed a meta-analysis to evaluate the association between the MCP-1 -2518A/G polymorp.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.25.1
- DOI:
- 10.4238/2015.May.25.1
Development of microsatellite markers for Hancornia speciosa Gomes (Apocynaceae)
Authors: A.J.L. Rodrigues, A.T. Yamaguishi, L.J. Chaves, A.S.G. Coelho, J.S. Lima and M.P.C. Telles
Herein, we describe 34 microsatellite loci developed using an enrichment genomic library for the tree species Hancornia speciosa Gomes (Apocynaceae). Thirty-five individuals were genotyped using 34 primers to analyze the polymorphisms at each locus. The number of alleles per locus ranged from 4 to 20. The average number of alleles was 8.11, and the expect.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.3.2
- DOI:
- 10.4238/2015.July.3.2
Biotechnological prospecting of foliar endophytic fungi of guaco (Mikania glomerata Spreng.) with antibacterial and antagonistic activity against phytopathogens
Authors: J.C. Polonio, T.T. Almeida, A. Garcia, G.E.G. Mariucci, J.L. Azevedo, S.A. Rhoden and J.A. Pamphile
Mikania glomerata (Spreng.), popularly known as “guaco”, is a plant from the Asteraceae family that has many therapeutic properties. The use of medicinal plants has been examined in studies on endophytic diversity and bioprospecting; endophytes inhabit the interior of plants without harming them. Microorganism-host complex interactions are rel.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.3.5
- DOI:
- 10.4238/2015.July.3.5
Characterization and expression analysis of microRNAs in Qira black sheep and Hetian sheep ovaries using Solexa sequencing
Authors: H. Shen, H.Y. Chen, B. Jia, G.H. Han, Y.S. Zhang and X.C. Zeng
The role of microRNAs (miRNAs) in the regulation of mammalian reproduction has been demonstrated previously. However, only a few studies have assessed the role of miRNAs in the reproduction processes of sheep. The elucidation of miRNA expression profiles in the ovaries of different sheep breeds representing fecundity extremes will be useful in understandi.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.3.11
- DOI:
- 10.4238/2015.July.3.11
Genetic diversity in intraspecific hybrid populations of Eucommia ulmoides Oliver evaluated from ISSR and SRAP molecular marker analysis
Authors: J. Yu, Y. Wang, M. Ru, L. Peng and Z.S. Liang
Eucommia ulmoides Oliver, the only extant species of Eucommiaceae, is a second-category state-protected endangered plant in China. Evaluation of genetic diversity among some intraspecific hybrid populations of E. ulmoides Oliver is vital for breeding programs and further conservation of this rare species. We studied the genetic diversity of 130 accessions.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.3.17
- DOI:
- 10.4238/2015.July.3.17
Boswellic acid attenuates asthma phenotype by downregulation of GATA3 via nhibition of PSTAT6
Authors: X. Zhou, J.G. Cai, W.W. Zhu, H.Y. Zhao, K. Wang and X.F. Zhang
To study the role of boswellic acid in reducing asthma phenotype severity and the relationship between the expression of pSTAT6 and GATA3, thirty-six mice were randomly divided into normal control group, asthma group, and boswellic acid group (treatment group). The asthma model was established through an intraperitoneal injection of sensitization liquid (.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.3.22
- DOI:
- 10.4238/2015.July.3.22
Effect of melatonin on oncosis of myocardial cells in the myocardial ischemia/reperfusion injury rat and the role of the mitochondrial permeability transition pore
Authors: L.F. Liu, Z.H. Qian, Q. Qin, M. Shi, H. Zhang, X.M. Tao and W.P. Zhu
We aimed to evaluate the effect of melatonin on myocardial cell oncosis in the myocardial ischemia/reperfusion injury rat, and the role of the mitochondrial permeability transition pore (MPTP) therein. Sprague Dawley rats (N = 60) were randomly divided into five groups of 12 rats each: control, ischemia/reperfusion (I/R), melatonin treatment (MT), melaton.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.3.24
- DOI:
- 10.4238/2015.July.3.24
Evaluation of genotoxicity of Pseudevernia furfuracea (L.) Zopf by RAPD analysis
Authors: S. Aras, T. Beyazta��, D. Cansaran-Duman and E. G�¶k�§e-G�¼nd�¼zer
We investigated the suitability and applicability of Pseudevernia furfuracea (L.) Zopf for environmental genotoxicity assessment. P. furfuracea lichen specimens were collected from 10 different Pinus species, in every 5 km, starting from around an iron-steel factory located in the central area of Karabük Province up to Ye.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.December.15.4
- DOI:
- 10.4238/2011.December.15.4
Genetic diversity analysis of tree peony germplasm using iPBS markers
Authors: Y.B. Duan, D.L. Guo, L.L. Guo, D.F. Wei and X.G. Hou
We examined the genetic diversity of 10 wild species (populations) and 55 varieties of tree peony using inter-primer binding site (iPBS) markers. From a total of 36 iPBS primers, 16 were selected based on polymorphic amplification. The number of bands amplified by each primer ranged from 9 to 19, with an average of 12.88 bands per primer. The length of ba.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.3.31
- DOI:
- 10.4238/2015.July.3.31
Construction and identification of pIRES2-NGF-VEGF165 bicistronic eukaryotic expression vector
Authors: B.N. Li, W.D. Li, H.G. Feng, J.T. Lin and Z.Q. Yuan
We used a simple and efficient method to construct the bicistronic eukaryotic expression vector pIRES2-NGF-VEGF165. The nerve growth factor (NGF) gene was obtained from the genomic DNA of human peripheral blood mononuclear cells by polymerase chain reaction. The NGF cDNA fragment was inserted into the multiple cloning sites of the pI.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.25.23
- DOI:
- 10.4238/2014.July.25.23