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Microbial Genetics

Microbial Genetics   Research Article

Determining the role of a probiotic in the restoration of intestinal microbial balance by molecular and cultural techniques

Authors: Affhan Shoaib, W. Dachang and Y. Xin

The human intestine has a vast variety of microorganisms, and their balance is dependent on several factors. Antibiotics affect microfloral balance and allow naturally opportunistic organisms to multiply. Azithromycin is the most widely used macrolide antibiotic, active against a wide number of pathogens including Pseudomonas aeruginosa and Staphylococcus.. Read More»

Genet. Mol. Res. 14(1):
2015.February.20.8
DOI:
10.4238/2015.February.20.8
Microbial Genetics   Research Article

Analysis of microbial diversity and niche in rhizosphere soil of healthy and diseased cotton at the flowering stage in southern Xinjiang

Authors: F.G. Luan, L.L. Zhang, Y.Y. Lou, L. Wang, Y.N. Liu and H.Y. Zhang

Understanding how microbial community composition and diversity respond to continuous cropping obstacle is not well understood. However, determining the community composition vs assessing the diversity of molecular operational taxonomic units is often difficult. In this study, we focused on the microbial diversity and niche differentiation in rhizosphere .. Read More»

Genet. Mol. Res. 14(1):
2015.March.6.7
DOI:
10.4238/2015.March.6.7
Microbial Genetics   Research Article

Characteristics of immune cell changes before and after immunotherapy and their clinical significance in patients with unexplained recurrent spontaneous abortion

Authors: L. Gao, J.P. Zhang, H. Chen, S.N. Zhang, L.B. Chen, J.P. Tan, M.L. Liu, L.L. Meng, Y.H. Wang, R. Zhang, Y.L. Liu and W.B. Cai

To investigate the characteristics of immune cells before and after immunotherapy and their clinical significance in patients with unexplained recurrent spontaneous abortion (URSA), an analysis of 67 URSA patients, 67 sporadic spontaneous abortion (SA) patients, and 22 normal nonpregnant women (as controls) was conducted. URSA.. Read More»

Genet. Mol. Res. 13(1):
http://dx.doi.org/2014.February.27.1
DOI:
http://dx.doi.org/10.4238/2014.February.27.1
Microbial Genetics   Research Article

Gene expression profile of the plant pathogen Fusarium graminearum under the antagonistic effect of Pantoea agglomerans

Authors: V. Pandolfi, E.C. Jorge, C.M.R. Melo, A.C.S. Albuquerque and H. Carrer

The pathogenic fungus Fusarium graminearum is an ongoing threat to agriculture, causing losses in grain yield and quality in diverse crops. Substantial progress has been made in the identification of genes involved in the suppression of phytopathogens by antagonistic microorganisms; however, limited information regarding respo.. Read More»

Genet. Mol. Res. 9(3):
vol9-3gmr828
DOI:
10.4238/vol9-3gmr828
Microbial Genetics   Research Article

Functional molecular markers (EST-SSR) in the full-sib reciprocal recurrent selection program of maize (Zea mays L.)

Authors: K.S.C. Galv�£o, H.C.C. Ramos, P.H.A.D. Santos, G.C. Entringer, J.C.F. Vettorazzi and M.G. Pereira

This study aimed to improve grain yield in the full-sib reciprocal recurrent selection program of maize from the North Fluminense State University. In the current phase of the program, the goal is to maintain, or even increase, the genetic variability within and among populations, in order to increase heterosis of the 13th cycle of reciprocal recurrent se.. Read More»

Genet. Mol. Res. 14(3):
2015.July.3.10
DOI:
10.4238/2015.July.3.10
Microbial Genetics   Research Article

Comparison of bacterial communities in the Solim�µes and Negro River tributaries of the Amazon River based on small subunit rRNA gene sequences

Authors: J.C.C. Peixoto, L. Leomil, J.V. Souza, F.B.S. Peixoto and S. Astolfi-Filho

The microbiota of the Amazon River basin has been little studied. We compared the structure of bacterial communities of the Solimões and Negro Rivers, the main Amazon River tributaries, based on analysis of 16S rRNA gene sequences. Water was sampled with a 3-L Van Dorn collection bottle; samples were collected at nine d.. Read More»

Genet. Mol. Res. 10(4):
Microbial Genetics   Research Article

Codon usage bias analysis for the spermidine synthase gene from Camellia sinensis (L.) O. Kuntze

Authors: E. You, Y. Wang, Z.T. Ding, X.F. Zhang, L.L. Pan and C. Zheng

The spermidine synthase (SPDS) gene exists widely in all types of plants. In this paper, the codon usage of the SPDS gene from Camellia sinensis (CsSPDS) was analyzed. The results showed that the codon usage of the CsSPDS gene is biased towards the T-ended or A-ended codons, which is similar to that observed in 73 genes selected from the C. sinensis genom.. Read More»

Genet. Mol. Res. 14(3):
2015.July.3.12
DOI:
10.4238/2015.July.3.12
Microbial Genetics   Research Article

rbcS SRS4 promoter from Glycine max and its expression activity in transgenic tobacco.

Authors: X.Y. Cui, Z.Y. Chen, L. Wu, X.Q. Liu, Y.Y. Dong, F.W. Wang and H.Y. Li

The regulatory region of the ribulose-1,5-bisphosphate carboxylase small subunit gene SRS4 from soybean (Glycine max) was cloned using TAIL-PCR and general PCR, and named the rbcS promoter. The promoter was fused with the GUS gene and introduced into Nicotiana tabacum via Agrobacterium-mediated leaf disk transformation. In 4-week-old transgenic tobacco pl.. Read More»

Genet. Mol. Res. 14(3):
2015.July.3.15
DOI:
10.4238/2015.July.3.15
Microbial Genetics   Research Article

Assessment of microsatellites in estimating inter- and intraspecific variation among Neotropical Crocodylus species

Authors: A. Bashyal, B.A. Gross, M. Venegas-Anaya, F. Lowrance and L.D. Densmore III

We tested microsatellites that were developed for the saltwater crocodile (Crocodylus porosus) for cross-species amplification and to provide an estimate of inter- and intraspecific variation among four species of Neotropical crocodiles (C. rhombifer, C. intermedius, C. acutus, and C. mo.. Read More»

Genet. Mol. Res. 13(3):
2014.July.25.2
DOI:
10.4238/2014.July.25.2
Microbial Genetics   Research Article

MicroRNAs as novel biomarkers for the differentiation of malignant versus benign thyroid lesions: a meta-analysis

Authors: G.J. Zhou, M. Xiao, L.N. Zhao, J.G. Tang and L. Zhang

The aim of this meta-analysis was to systematically evaluate the diagnostic accuracy of microRNAs (miRNAs) in distinguishing malignant thyroid lesions from benign ones and to determine the potential of miRNAs as diagnostic biomarkers for thyroid cancer. The random-effect model was used to summarize the pooled estimates of diagnostic accuracy, including se.. Read More»

Genet. Mol. Res. 14(3):
2015.July.3.3
DOI:
10.4238/2015.July.3.3