Microbial Genetics
Efficacy of oral administration of lactic acid bacteria isolated from cocoa in a fermented milk preparation: reduction of colitis in an experimental rat model
Authors: T.F. dos Santos, T.A. Melo, D.S. Santos, R.P. Rezende, J.C.T. Dias and C.C. Romano
We investigated the probiotic potential of lactic acid bacteria (LAB) obtained from cocoa fermentation using an experimental rat model of colitis. Cocoa beans were collected from fermentation boxes every 12 h for 5 days to isolate the microorganisms. Strains were isolated by serial dilution and plating on MRS agar. Gram-positive and catalase-negative rods.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038097
- DOI:
- 10.4238/gmr.15038097
Expression of the porcine lipoic acid synthase (LIAS) gene in Escherichia coli
Authors: W.F. Lu, J.J. Cao, Y.J. Guo, K. Zhong, G.M. Zha, L.F. Wang and G.Y. Yang
Lipoic acid synthase, which exists primarily in mitochondria, participates in the biosynthesis of intrinsic lipoic acid. The lipoic acid synthase gene in pig is known as LIAS. To further investigate the biological functions of the protein that is encoded by LIAS, we cloned the open read frame of porcine LIAS (GenBank No. JN797612.1) into.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.24.16
- DOI:
- 10.4238/2014.July.24.16
Alkaline phosphatase activity in salivary gland cells of Rhodnius neglectus and R. prolixus (Hemiptera, Triatominae)
Authors: A.P.M. Lima-Oliveira, K.C.C. Alevi, A.C.B. Anh�ª and M.T.V. Azeredo-Oliveira
Alkaline phosphatase activity was detected in salivary gland cells of the Rhodnius neglectus Lent, 1954, and R. prolixus Stal, 1859, vectors of Trypanosoma cruzi Chagas, 1909 (etiological agent of Chagas disease) and T. rangeli Tejera, 1920 (pathogenic to insect). The Gomori technique was used to demonstrate alkaline phosphatase activity. Alkaline phospha.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038339
- DOI:
- 10.4238/gmr.15038339
Effect of karst rocky desertification on soil fungal communities in Southwest China
Authors: P.C. Wang, B.T. Mo, Y. Chen, Q.F. Zeng and L.B. Wang
Karst mountainous ecosystems are associated with karst rocky desertification (KRD), which can greatly impact soil structure and function. Despite the importance of soil microbes as a major factor maintaining ecosystem stability, we know little about the effect on soil fungal communities of KRD in karst regions. We investigated this relationship across a g.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038460
- DOI:
- 10.4238/gmr.15038460
Fatty acid composition evaluation of edible parts of Eriocheir sinensis in intensive ponds of Gucheng waters
Authors: J.Y. Yu and P.S. Yan
In Gucheng Lake, Jiangsu Province, China, randomly selected crabs were fed 2 diet types. Crab crude oil methyl ester analysis was performed using gas chromatography-mass spectrometry. Fatty acid compositions in the 3 edible parts of a crab - the hepatopancreas, gonad, and muscle - were analyzed. C16:1 and C18:2 were significantly higher in most commercial.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.22.4
- DOI:
- 10.4238/2015.May.22.4
Streptomyces ansochromogenes Tur-10 produces a substance with antifungal bioactivity
Authors: N.M. Vasconcelos, J.M. Fontes, M.R.C.R. Lins, G.R.B. Bernardo,J.M. Ara�ºjo and G.M.S. Lima
The increased incidence of fungal infections and the development of drug resistance have led to the search for microorganisms capable of producing bioactive metabolites with antifungal activity. Among these microorganisms, Streptomyces spp are distinguished mainly owing to their potential to secrete bioactive molecules. The aim of this study was to evalua.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.22.13
- DOI:
- 10.4238/2015.May.22.13
Evaluation of antimicrobial activity of endophytic fungi from Camptotheca acuminata (Nyssaceae)
Authors: T. Ding, T. Jiang, J. Zhou, L. Xu and Z.M. Gao
Agricultural research of plant-derived endophytic fungi has grown in recent decades. We isolated 26 endophytic fungi from the leaves, stems and fruits of “the tree of life”, Camptotheca acuminata, and tested them for antimicrobial activities based on growth inhibition measurements in a modified agar diffusion metho.. Read More»
- Genet. Mol. Res. 9(4):
- vol9-4gmr809
- DOI:
- 10.4238/vol9-4gmr809
Genetic polymorphism, molecular characterization and relatedness of Macrobrachium species (Palaemonidae) based on RAPD-PCR
Authors: A.L. Guerra, A.V.B. Lima, F.G. Taddei and L. Castiglioni
The prawn genus Macrobrachium belongs to the family Palaemonidae. Its species are widely distributed in lakes, reservoirs, floodplains, and rivers in tropical and subtropical regions of South America. Globally, the genus Macrobrachium includes nearly 210 known species, many of which have economic and ecological importance. We .. Read More»
- Genet. Mol. Res. 9(4):
- vol9-4gmr935
- DOI:
- 10.4238/vol9-4gmr935
Phylogenetic analysis of endophytic bacterial isolates from leaves of the medicinal plant Trichilia elegans A. Juss. (Meliaceae)
Authors: S.A. Rhoden, A. Garcia, M.C. Santos e Silva, J.L. Azevedo and J.A. Pamphile
Various organisms such as fungi and bacteria can live inside plants, inhabiting the aerial parts (primarily the leaves) without causing damage. These microorganisms, called endophytes, produce an extensive variety of compounds that can be useful for medical and agronomic purposes. Trichilia elegans A. Juss., belonging to the Meliaceae family, shows wide d.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.February.20.7
- DOI:
- 10.4238/2015.February.20.7
An efficient method for DNA extraction from Cladosporioid fungi
Authors: M.A. Moslem, A.H. Bahkali, K.A. Abd-Elsalam and P.J.G.M. Wit
We developed an efficient method for DNA extraction from Cladosporioid fungi, which are important fungal plant pathogens. The cell wall of Cladosporioid fungi is often melanized, which makes it difficult to extract DNA from their cells. In order to overcome this we grew these fungi for three days on agar plates and extracted D.. Read More»
- Genet. Mol. Res. 9(4):
- vol9-4gmr936
- DOI:
- 10.4238/vol9-4gmr936