Abstract
Isolation and characterization of microsatellite loci in the purpleback flying squid (Sthenoteuthis oualaniensis)
Author(s): L. Lin, P. Li, Z.Z. Chen, Y.Y. Xiao, S.N. Xu, Y. Liu and C.H. LiThe purpleback flying squid (Sthenoteuthis oualaniensis) is a pelagic squid with tremendous potential for commercial exploitation. We isolated and characterized 21 polymorphic microsatellite loci for S. oualaniensis using a (GT)13-enriched genomic library. The number of alleles per locus varied from 6 to 32. The observed and expected heterozygosities ranged from 0.188 to 0.890, and 0.537 to 0.968, respectively. No significant linkage disequilibrium was detected at these loci. Five loci significantly deviated from the Hardy-Weinberg equilibrium, and four loci may have exhibited null alleles. These microsatellite markers will facilitatefurther studies in population genetics and the sustainable utilization of S. oualaniensis.
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