Microbial Genetics
Molecular phylogeny and a taxonomic proposal for the genus Streptococcus
Authors: F. P�³ntigo, M. Moraga and S.V. Flores
Alternative phylogenies for the genus Streptococcus have been proposed due to uncertainty about the among-species group relationships. Here, we performed a phylogenetic analysis of the genus Streptococcus, considering all the species groups and also the genomic data accumulated by other studies. Seventy-five species were subjected to a Bayesian phylogenet.. Read More»
- Genet. Mol. Res. 14(3):
- http://dx.doi.org/2015.September.21.1
- DOI:
- http://dx.doi.org/10.4238/2015.September.21.1
Expression and genomic integration of transgenes after Agrobacterium-mediated transformation of mature barley embryos
Authors: C. U�§arl�±, F. Tufan and F. G�¼rel
Mature embryos in tissue cultures are advantageous because of their abundance and rapid germination, which reduces genomic instability problems. In this study, 2-day-old isolated mature barley embryos were infected with 2 Agrobacterium hypervirulent strains (AGL1 and EHA105), followed by a 3-day period of co-cultivation in the presence of L-cystein amino .. Read More»
- Genet. Mol. Res. 14(1):
- 2015.February.6.13
- DOI:
- 10.4238/2015.February.6.13
Identification of BPI protein produced in different expression system and its association with Escherichia coli F18 susceptibility
Authors: Z.C. Wu, Y. Liu, W.H. Dong, S.Y. Sun, G.Q. Zhu, S.L. Wu and W.B. Bao
The super antibiotic bactericidal/permeability-increasing (BPI) protein is a member of a new generation of proteins that have been implicated as endotoxin-neutralizing agents. In this study, recombinant porcine BPI protein was obtained by generating porcine BPI encoding prokaryotic, eukaryotic, and yeast expression vectors. Recombinant protein expres.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.February.6.15
- DOI:
- 10.4238/2015.February.6.15
Microbial diversity in the larval gut of field and laboratory populations of the sugarcane weevil Sphenophorus levis (Coleoptera, Curculionidae)
Authors: R. Rinke, A.S. Costa, F.P.P. Fonseca, L.C. Almeida, I. Delalibera J�ºnior and F. Henrique-Silva
The sugarcane weevil, Sphenophorus levis, is a wide-spread sugarcane pest in Brazil. Sphenophorus levis may depend on microorganisms that inhabit its intestinal tract. We examined the diversity of the gut microbiota of S. levis, which was characterized using culture-dependent and culture-independent methods. Analysis of 16S rR.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.November.1.1
- DOI:
- 10.4238/2011.November.1.1
Isolation and characterization of 24 polymorphic microsatellite loci in Sepioteuthis lessoniana
Authors: X.-D. Zheng Z.-R. Zuo X.-J. Su Q. Li
Owing to their codominant, multiallelic, and highly polymorphic nature, microsatellite markers have been used widely in population genetics and biological resource conservation studies. To investigate the genetic structure of Sepioteuthis lessoniana, we developed 24 microsatellite DNA markers and assessed the polymorphism of each locus in a wild S. lessonian.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.November.14.3
- DOI:
- 10.4238/2012.November.14.3
Multiple antimicrobial resistance in Enterobacteriaceae isolates from pristine freshwater
Authors: Lima-Bittencourt CI, Cursino L, Gon�§alves-Dornelas H, Pontes DS, Nardi RMD, Callisto M, Chartone-Souza E and Nascimento AMA
A freshwater enterobacterial population (N = 111) was studied for antimicrobial and mercury resistance patterns, and for its possible association with biotic and abiotic factors in that environment. Conventional biochemical tests identified Klebsiella sp, Morganella sp, Serratia sp, Escherichia sp, Enterobacter sp, Edwarsiella.. Read More»
- Genet. Mol. Res. 6(3):
Selection and validation of reference houseÃ?Âkeeping genes in the J774A1 macrophage cell line for quantitative real-time PCR
Authors: F.B. Ferraz and J.H. Fernandez
Macrophages are essential components of the innate and adaptive immune responses, playing a decisive role in atherosclerosis, asthma, obesity, and cancer. The differential gene expression resulting from adhesion of macrophages to the extra-cellular matrix (ECM) has been studied in the J774A1 murine macrophage cell line using quantitative polymerase chain .. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017720
- DOI:
- 10.4238/gmr.15017720
Cloning and expression of ethylene receptor ERS1 at various developmental and ripening stages of mango fruit
Authors: C.A. Contreras-Vergara N.A. Stephens-Camacho G. Yepiz-Plascencia G.A. Gonz�¡lez-Aguilar A.A. Arvizu-Flores E. Sanchez-Sanchez M.A. Islas-Osuna
Ethylene induces characteristic ripening reactions in climacteric fruits through its binding to histidine-kinase (HK) receptors, activating the expression of ripening genes. Ethylene receptors have been found in Arabidopsis thaliana (Brassicaceae) and some fruits; number and expression patterns differ among species. In mango, only ethylene receptor ETR1 was .. Read More»
- Genet. Mol. Res. 11(4):
- 2012.September.10.6
- DOI:
- 10.4238/2012.September.10.6
Microbial community and performance of slaughterhouse wastewater treatment filters
Authors: M.I. Stets, R.M. Etto, C.W. Galv�£o, R.A. Ayub, L.M. Cruz, M.B.R. Steffens and A.C. Barana
The performance of anaerobic filter bioreactors (AFs) is influenced by the composition of the substrate, support medium, and the microbial species present in the sludge. In this study, the efficiency of a slaughterhouse effluent treatment using three AFs containing different support media was tested, and the microbial diversit.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.June.16.3
- DOI:
- http://dx.doi.org/10.4238/2014.June.16.3
Genes involved in glucose repression and oxidative stress response in the fission yeast Schizosaccharomyces pombe
Authors: K.G. Suslu, B. Palabiyik and G. Temizkan
We looked for changes in gene expression and novel genes that could be involved in the interaction between glucose repression and oxidative stress response in the fission yeast, Schizosaccharomyces pombe, using a constitutive invertase mutant, ird11, which is resistant to glucose. BLAST analysis was made of the S. pombe genome.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.November.8.4
- DOI:
- 10.4238/2011.November.8.4