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Microbial Genetics

Microbial Genetics   Research Article

Chromosome number, microsporogenesis, microgametogenesis, and pollen viability in the Brazilian native grass Mesosetum chaseae (Poaceae)

Authors: L.A.C. Silva M.S. Pagliarini S.A. Santos N. Silva V.F. Souza

The genus Mesosetum is a primarily South American genus with 42 species. Mesosetum chaseae, regionally known as âÂ?Â?grama-do-cerradoâÂ?Â?, is abundant in the Pantanal Matogrossense (Brazil); it is a valuable resource for livestock and for environmental conservation. We collected specimens from the Nhecolandia sub-region of the Brazilian Pantanal, located .. Read More»

Genet. Mol. Res. 11(4):
2012.September.12.1
DOI:
10.4238/2012.September.12.1
Microbial Genetics   Research Article

Genetic diversity of endangered Polyporus umbellatus from China assessed using a sequence-related amplified polymorphism technique

Authors: Y. Zhang Y. Kang Y. Qin Z. Zhou M. Lei H. Guo

Polyporus umbellatus (Pers.) Fries is an endangered medicinal fungus in China with in vivo anticancer activity, but its genetic information is lacking. Eight natural P. umbellatus strains collected from 7 provinces in China were subjected to sequence-related amplified polymorphism markers to estimate the level and pattern of genetic diversity. Forty-nine .. Read More»

Genet. Mol. Res. 11(4):
2012.December.3.1
DOI:
10.4238/2012.December.3.1
Microbial Genetics   Research Article

Assessing genetic divergence in interspecific hybrids of Aechmea gomosepala and A. recurvata var. recurvata using inflorescence characteristics and sequence-related amplified polymorphism markers

Authors: F. Zhang Y.Y. Ge W.Y. Wang X.L. Shen X.Y. Yu

Conventional hybridization and selection techniques have aided the development of new ornamental crop cultivars. However, little information is available on the genetic divergence of bromeliad hybrids. In the present study, we investigated the genetic variability in interspecific hybrids of Aechmea gomosepala and A. recurvata var. recurvata using inflorescen.. Read More»

Genet. Mol. Res. 11(4):
2012.September.27.2
DOI:
10.4238/2012.September.27.2
Microbial Genetics   Research Article

Molecular characterization of the endophytic fungal community associated with Eichhornia azurea (Kunth) and Eichhornia crassipes (Mart.) (Pontederiaceae) native to the Upper Paran�¡ River floodplain, Brazil

Authors: T.T. Almeida, R.C. Orlandelli, J.L. Azevedo and J.A. Pamphile

Endophytic fungi live in the interior of healthy plants without causing them any damage. These fungi are of biotechnological interest; they may be used in the biological control of pests and plant diseases, and in the pharmaceutical industry. The aquatic macrophytes Eichhornia azurea (Kunth) and Eichhornia crassipes (Mart.) belong to the Pontederiaceae fa.. Read More»

Genet. Mol. Res. 14(2):
2015.May.11.25
DOI:
10.4238/2015.May.11.25
Microbial Genetics   Research Article

Bioinformatic analysis and characteristics of glycoprotein C encoded by the newly identified UL44 gene of duck plague virus

Authors: K.F. Sun, A.C. Cheng, M.S. Wang

Glycoprotein C is one of the duck plague virus (DPV) glycoproteins and is encoded by the DPV UL44 gene. DPV glycoprotein C (DPV-gC) comprises 431 amino acids with a putative molecular mass of 47.35 kDa. Sequence analysis indicated that the protein possesses typical characteristics of type-I membrane glycoproteins, containing a.. Read More»

Genet. Mol. Res. 13(2):
http://dx.doi.org/2014.June.17.2
DOI:
http://dx.doi.org/10.4238/2014.June.17.2
Microbial Genetics   Research Article

Salt stress represses production of extracellular proteases in Bacillus pumilus.

Authors: R.F. Liu, C.L. Huang and H. Feng

Bacillus pumilus is able to secrete subtilisin-like proteases, one of which has been purified and characterized biochemically, demonstrating great potential for use in industrial applications. In the current study, the biosynthesis and transcription of extracellular proteases in B. pumilus (BA06) under salt stress were investigated using various methods, .. Read More»

Genet. Mol. Res. 14(2):
2015.May.11.27
DOI:
10.4238/2015.May.11.27
Microbial Genetics   Research Article

Genome sequencing and systems biology analysis of a lipase-producing bacterial strain

Authors: N. Li*, D.D. Li*, Y.Z. Zhang, Y.Z. Yuan, H. Geng, L. Xiong and D.L. Liu

Lipase-producing bacteria are naturally-occurring, industrially-relevant microorganisms that produce lipases, which can be used to synthesize biodiesel from waste oils. The efficiency of lipase expression varies between various microbial strains. Therefore, strains that can produce lipases with high efficiency must be screened, and the conditions of lipas.. Read More»

Genet. Mol. Res. 15(1):
gmr.15017331
DOI:
10.4238/gmr.15017331
Microbial Genetics   Research Article

Codon optimization enhances the expression of porcine �²-defensin-2 in Escherichia coli

Authors: C.Y. Gao, T.T. Xu, Q.J. Zhao and C.L. Li

Porcine β-defensin-2 (pBD2) is a cationic antimicrobial peptide that has therapeutic potential. The amount of pBD2 in nature is limited, and the expression of pBD2 in Escherichia coli is low, probably because a different gene codon is used by prokaryotic organisms to that used by eukaryotes. Codon preference optimization is one of the ways to increas.. Read More»

Genet. Mol. Res. 14(2):
2015.May.12.1
DOI:
10.4238/2015.May.12.1
Microbial Genetics   Research Article

Simple DNA extraction protocol for a 16S rDNA study of bacterial diversity in tropical landfarm soil used for bioremediation of oil waste

Authors: B.M. Maciel A.C.F. Santos J.C.T. Dias R.O. Vidal R.J.C. Dias E. Gross J.C.M. Cascardo R.P. Rezende

Landfarm soil is used to bioremediate oil wastes from petrochemical industries. We developed a simplified protocol for microbial DNA extraction of tropical landfarm soil using only direct lysis of macerated material. Two samples of tropical landfarm soil from a Brazilian refinery were analyzed by this protocol (one consisted o.. Read More»

Genet. Mol. Res. 8(1):
vol8-1gmr559
DOI:
10.4238/vol8-1gmr559
Microbial Genetics   Research Article

Karyotype and spermatogenesis in Triatoma lenti (Hemiptera: Triatominae), a potential Chagas vector

Authors: K.C.C. Alevi P.P. Mendon�§a M. Succi N.P. Pereira J.A. Rosa M.T.V. Azeredo-Oliveira

All species of Triatominae are susceptible to infection by Trypanosoma cruzi (Kinetoplastida: Trypanosomatidae) and consequently, potential insect vectors of Chagas disease. Currently, there are 140 known species of triatomine bugs, which can be grouped into specific species complexes. The species Triatoma lenti (Hemiptera: Triatominae) is found only in B.. Read More»

Genet. Mol. Res. 11(4):
2012.December.17.3
DOI:
10.4238/2012.December.17.3