Microbial Genetics
Can established cultured papilloma cells harbor bovine papillomavirus?
Authors: S.R.C. Campos1, C. Trindade1, O.P. Ferraz1, D.N.S. Giovanni1, A.A. Lima1,2, H.V.A. Caetano1,3, R.F. Carvalho1,3, E.H. Birgel Jr.4, M.L.Z. Dagli3, E. Mori5, P.E. Brand�£o5, L.J. Richtzenhain5, W. Be�§ak1,6 and R.C. Stocco1
Papillomaviruses have been reported to be very difficult to grow in cell culture. Also, there are no descriptions of cell cultures from lesions of bovine cutaneous papillomatosis, with identification of�� � � � � � � �� different bovine papilloma virus (BPV) DNA sequences. In the present report, we describ.. Read More»
- Genet. Mol. Res. 7(4):
- vol7-4gmr498
- DOI:
- 10.4238/vol7-4gmr498
Optimization of dengue virus genome assembling using GSFLX 454 pyrosequencing data: evaluation of assembling strategies
Authors: S.M.M. Casseb J.F. Cardoso R. Ramos A. Carneiro M. Nunes P.F.C. Vasconcelos A. Silva
Currently assembling genomes without reference is one of the most important challenges for bioinformaticists all over the world in an attempt to characterize new organisms. The current study has used two dengue virus type 4 (DENV-4) strains recently isolated in Brazil, which have its genomes sequenced using the GSFLX 454 seque.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.August.17.6
- DOI:
- 10.4238/2012.August.17.6
Distribution of food-borne Staphylococcus aureus enterotoxin genes
Authors: W.D. Hu
We identified and analyzed 5 new-type enterotoxin genes, including SEj, SEl, SEq, SEm, and SEr, to explore the distribution of 5 enterotoxin genes in Staphylococcus aureus of different origins as well as their correlations and differences. We examined the distribution of the S. aureus enterotoxin genes and their pathogenic mechanisms. A total of 660 speci.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15016084
- DOI:
- 10.4238/gmr.15016084
Eucalyptus growth promotion by endophytic Bacillus spp
Authors: I.C.P. Paz R.C.M. Santin A.M. Guimar�£es O.P.P. Rosa A.C.F. Dias M.C. Quecine J.L. Azevedo A.T.S. Matsumura
Clonal eucalyptus plantings have increased in recent years; however, some clones with high production characteristics have vegetative propagation problems because of weak root and aerial development. Endophytic microorganisms live inside healthy plants without causing any damage to their hosts and can be beneficial, acting as plant growth promoters. We is.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.August.17.9
- DOI:
- 10.4238/2012.August.17.9
Cross-reactivity of anti-human cytokine monoclonal antibodies used as a tool to identify novel immunological biomarkers in domestic ruminants
Authors: C.S. Bao, L. Liu, B. Wang, X.-G. Xia, Y.J. Gu, D.J. Li, S.L. Zhan,G.L. Chen and F.B. Yang
Eleven commercially available PE-labeled anti-human (IL-1-α, IL-6, IL-8, TNF-α, IL-17A, IL-5, IL-10, IL-12 and IL-13) and anti-mouse (IL-10, TNF-α) cytokine monoclonal antibodies (mAbs) were tested for cross-reactivity with cattle, goat, and sheep cytokines. Cross-reactivity was assessed by comparative analysis with the standard reactivi.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.February.3.2
- DOI:
- 10.4238/2015.February.3.2
Gene expression of antioxidant enzymes and coffee seed quality during pre- and post-physiological maturity
Authors: F.C. Santos, F. Caixeta, A.C.S. Clemente, E.V. Pinho and S.D.V.F. Rosa
Seeds collected at different maturation stages vary in physiological quality and patterns of protective antioxidant systems against deterioration. In this study we investigated the expression of genes that codify catalase (CAT), dismutase superoxide (SOD), and polyphenol oxidase (PPO) during the pre- and post-physiological maturation phases in whole seeds.. Read More»
- Genet. Mol. Res. 13(4):
- 2014
- DOI:
- 10.4238/2014
Population genetic analysis and trichothecene profiling of Fusarium graminearum from wheat in Uruguay
Authors: D. Pan1, A. Mionetto1, N. Calero1, M.M. Reynoso2, A. Torres2 and L. Bettucci1
Fusarium graminearum sensu stricto (F. graminearum s.s.) is the major causal agent of Fusarium head blight of wheat worldwide, and contaminates grains with trichothecene mycotoxins that cause serious threats to food safety and animal health. An important aspect of managing this pathogen and reducing mycotoxin contamination of wheat is knowledge regarding .. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017270
- DOI:
- 10.4238/gmr.15017270
Virulence characteristics and antimicrobial susceptibility of uropathogenic Escherichia coli strains
Authors: F.A. Oliveira, K.S. Paludo, L.N.V.S. Arend, S.M.S.S. Farah, F.O. Pedrosa, E.M. Souza, M. Surek, G. Picheth and C.M.T. Fadel-Picheth
Eight virulence factors associated with uropathogenic Escherichia coli (UPEC) were investigated in 204 clinical isolates of E. coli recovered from urine cultures at counts ≥105. The bacteria were classified into two groups according to the number of leukocytes in urine samples from which they were isolated: group I ≤8 le.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.October.31.5
- DOI:
- 10.4238/2011.October.31.5
A rapid and sensitive loop-mediated isothermal amplification procedure (LAMP) for Mycoplasma hyopneumoniae detection based on the p36 gene
Authors: M.J. Liu, G.M. Du, F.F. Bai, Y.Z. Wu, Q.Y. Xiong, Z.X. Feng, B. Liand G.Q. Shao
The aim of this study was to establish a method for sensitive and rapid diagnosis of Mycoplasma hyopneumoniae in clinical specimens. To this effect, we employed three sets of primers specifically designed for amplification of nucleic acids under isothermal conditions. After optimization of reaction conditions, M. hyopneumoniae could be successfully detect.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.4.27
- DOI:
- 10.4238/2015.May.4.27
Low polymorphism revealed in new microsatellite markers for Bemisia tabaci (Hemiptera: Aleyrodidae)
Authors: N. Jiang Y.H. Zheng X.J. Chen C. Qiu X.F. Zhang S.H. Wen G.X. Bian
Suppression subtractive hybridization was used to analyze differential expression of genes in rat peritoneal macrophages after granulocyte macrophage colony-stimulating factor treatment. We identified and cloned the mouse C10 analog gene in the rat, and named it as ccl6. The full-length cDNA of rat ccl6 was 467 bp, which contains a single-open reading frame .. Read More»
- Genet. Mol. Res. 11(4):
- 2012.November.12.6
- DOI:
- 10.4238/2012.November.12.6