Microbial Genetics
Molecular detection by analysis of the 16S rRNA gene of fecal coliform bacteria from the two Korean Apodemus species (Apodemus agrarius and A. peninsulae)
Authors: M.M. Rahman, K.B. Yoon, S.J. Lim, M.G. Jeon, H.J. Kim, H.Y. Kim, J.Y. Cho, H.M. Chae and Y.C. Park
Wild mouse feces can disseminate zoonotic microorganisms throughout a farm, which is a great threat to human health and can lead to economic loss through contaminated agricultural produce. To assess the microbial communities, especially fecal coliform bacteria, we used two methods. First, we isolated bacterial colonies onto th.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029510
- DOI:
- 10.4238/gmr16029510
Recombinant expression and characterization of an endoglucanase III (cel12a) from Trichoderma harzianum (Hypocreaceae) in the yeast Pichia pastoris
Authors: W.C. Generoso, W. Malag�³-Jr., N. Pereira Jr. and F. Henrique-Silva
Filamentous fungi from the genus Trichoderma have been widely investigated due to their considerable production of important biotechnological enzymes. Previous studies have demonstrated that the T. harzianum strain IOC-3844 has a high degree of cellulolytic activity. After excluding the native signal peptide, the open reading .. Read More»
- Genet. Mol. Res. 11(2):
- dx.doi.org/2012.May.21.11
- DOI:
- dx.doi.org/10.4238/2012.May.21.11
Analysis of Antarctic proteobacteria by PCR fingerprinting and screening for antimicrobial secondary metabolites
Authors: L.-H. Lee, Y.-K. Cheah, A.M. Nurul Syakima, M.S. Shiran, Y.-L. Tang, H.-P. Lin and K. Hong
Fifty-seven proteobacterium species were successfully isolated from soils of Barrientos Island of the Antarctic using 11 different isolation media. Analysis of 16S rDNA sequencing of these isolates showed that they belonged to eight different genera, namely Bradyrhizobium, Sphingomonas, Methylobacterium, Caulobacter, Paracoccu.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.June.15.12
- DOI:
- 10.4238/2012.June.15.12
Allele frequencies of microsatellite loci for genetic characterization of a Sicilian bovine population
Authors: M. Cosenza, S. Reale, T. Lupo, F. Vitale and S. Caracappa
Short tandem repeats are used as an effective method to trace DNA markers in genotyping. Using a standardized kit, we tested 11 microsatellite markers recommended by the International Society for Animal Genetics (ISAG) in a sample of 495 Sicilian cattle. The aim of this study was to investigate the allele frequencies in the Sicilian cattle population to prov.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.January.30.12
- DOI:
- 10.4238/2015.January.30.12
Elevated micronucleus frequencies in patients with pleural plaque secondary to environmental exposure to asbestos
Authors: M. G�¶vercin, A.G. Tomat�±r, F. Evyapan, �°. Acikbas, G. Co��kun and B. Akdag
We examined genotoxic damage and frequency of micronuclei in people living in the Bekilli and Suller districts of Denizli city who had been diagnosed with pleural plaques as a result of environmental exposure to asbestos. Peripheral blood samples were obtained from 30 volunteer patients 59-86 years old who did not smoke or con.. Read More»
- Genet. Mol. Res. 13(1):
- 2014.January.28.5
- DOI:
- 10.4238/2014.January.28.5
Comparison of extraction methods of total microbial DNA from freshwater
Authors: P. Li, S.F. Yang, B.B. Lv, K. Zhao, M.F. Lin, S. Zhou, X. Song and X.M. Tang
The demand for molecular analysis of aquatic microbial communities in freshwater has highlighted the need for efficient methods of DNA extraction. The centrifugation method and filtration-membrane method are 2 widely used methods for extracting DNA. The objective of this study was to compare the extraction efficiency of 3 methods, including the centrifugatio.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.January.30.16
- DOI:
- 10.4238/2015.January.30.16
Analysis of genetic diversity of salt-tolerant alfalfa germplasms
Authors: J. Jiang, B.L. Yang, T. Xia, S.M. Yu, Y.N. Wu, H. Jin and J.R. Li
Random amplified polymorphic DNA technology was used to analyze the genetic diversity of 25 salt-tolerant alfalfa varieties using 30 different primers. Results showed that the percentage of polymorphic loci between single-plant DNA was 81.52%, and that between mixed DNA of various varieties was 61.65%. Compared to the mixed DNA samples, single-plant DNA s.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.4.1
- DOI:
- 10.4238/2015.May.4.1
Phylogeographic analysis of African swine fever virus based on the p72 gene sequence
Authors: Y. Muangkram, M. Sukmak and W. Wajjwalku
African swine fever virus (ASFV) outbreak has been considered as an emerging and re-emerging disease for almost a century. Diagnostically, simple polymerase chain reaction and sequencing-based molecular detection could be employed for both viral identification and genotyping. This study established a novel phylogenetic analysis and epidemiology comparison.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.4.15
- DOI:
- 10.4238/2015.May.4.15
Analysis and comparison of clinicohematological parameters and molecular and cytogenetic response of two Bcr/Abl fusion transcripts
Authors: S. Polampalli1, A. Choughule2, N. Negi1, S. Shinde2, C. Baisane3, P. Amre3, P.G. Subramanian2, S. Gujral2, K. Prabhash1 and P. Parikh1
Different forms of p210 are produced by alternative splicing, namely b2a2 and b3a2. There have been many contrasting data establishing a relationship between the two Bcr/Abl transcripts and platelet counts and also response to treatment. However, the data published to date have been on a small group of patients. The aim of the present study was to determi.. Read More»
- Genet. Mol. Res. 7(4):
- vol7-4gmr485
- DOI:
- 10.4238/vol7-4gmr485
Genetic variability analysis of Zymomonas mobilis strains from the UFPEDA microorganisms collection
Authors: L.C.N. Silva, J.M. Ara�ºjo, J.L. Azevedo, R.J.S.A. Padilha and R. Yara
Zymomonas mobilis is a Gram-negative bacterium that has drawn attention in the bioethanol industry. Besides bioethanol, this bacterium also produces other biotechnological products such as levans, which show antitumor activity. Molecular studies involving Z. mobilis have advanced to the point that allows us to characterize interspecies genetic diversity a.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.February.2.5
- DOI:
- 10.4238/2015.February.2.5