Microbial Genetics
Effects of stingless bee and honey bee propolis on four species of bacteria
Authors: A.P. Farnesi, R. Aquino-Ferreira, D. De Jong, J.K. Bastosand A.E.E. Soares
We examined the antibacterial activities of several types of propolis, including Africanized honey bee green propolis and propolis produced by meliponini bees. The antibacterial activity of green propolis against Micrococcus luteus and Staphylococcus aureus was superior to that of Melipona quadrifasciata and Scaptotrigona sp p.. Read More»
- Genet. Mol. Res. 8(2):
- vol8-2kerr023
- DOI:
- 10.4238/vol8-2kerr023
Vero cells infected with the Lederle strain of canine distemper virus have increased Fas receptor signaling expression at 15 h post-infection
Authors: H.L. Del Puerto, A.S. Martins, G.F. Braz, F. Alves, M.B. Heinemann, D.S. Raj�£o, F.C. Ara�ºjo, S.F. Martins, D.R. Nascimento, R.C. Leite and A.C. Vasconcelos
We evaluated the expression of the Fas receptor gene in Vero cells infected with the Lederle vaccine strain of canine distemper virus using RT-PCR. Vero cells were plated, and after being grown for 24 h in MEM with 5% FBS, 80-90% confluent monolayer cultures were infected with the virus. The cells were harvested at 3, 6, 9, an.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.October.18.3
- DOI:
- 10.4238/2011.October.18.3
Detection of Salmonella Enteritidis in asymptomatic carrier animals: comparison of quantitative real-time PCR and bacteriological culture methods
Authors: B.M. Maciel, J.C.T. Dias, C.C. Romano, N. Sriranganathan, M. Brendel and R.P. Rezende
Quantification of Salmonella in asymptomatic carrier animals can be used to assess microbial risk and monitor the level of contamination in domestic animals used for food production. We examined the sensitivity, specificity and accuracy of real-time qPCR, without pre-enrichment or selective enrichment stages, for the quantific.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.October.24.1
- DOI:
- 10.4238/2011.October.24.1
A novel set of single-copy nuclear DNA markers for the genetic study of Salicaceae
Authors: S.H. Du, Z.S. Wang and J.G. Zhang
Species of Populus are widely distributed worldwide, playing a significant role in both ecology and economy. However, the lack of single-copy nuclear markers limits knowledge about the phylogeny and population genetics of this genus. In the present study, primer pairs of 15 single-copy nuclear markers were developed t.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.4.5
- DOI:
- 10.4238/2014.July.4.5
Evolution of CD4+CD25hi T cell subsets in Aspergillus-infected liver transplantation recipients reduces the incidence of transplantation rejection via upregulating the production of anti-inflammatory cytokines
Authors: T. Xing, L. Zhong, G. Qiu, L. Huang and Z. Peng
Infection is the leading risk factor of liver transplantation-related death. Aspergillosis is a life-threatening complication in immune-compromised patients, and is the cause of approximately 2/3 of deaths in liver transplant recipients. In our previous studies, we found a regulatory T cell (Treg) population that showed signif.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.4.7
- DOI:
- 10.4238/2014.July.4.7
Effect of different compounds on the induction of laccase production by Agaricus blazei
Authors: J.S. Valle, L.P.S. Vandenberghe,A.C.C. Oliveira, M.F. Tavares,G.A. Linde, N.B. Colauto, C.R. Soccol
Laccases are polyphenol oxidases produced by many fungi and have many applications in textile, food and beverage, and pulp and paper industries. Laccase production can be induced using aromatic or phenolic compounds that mostly affect the transcription of laccase-encoding genes. In this study, we analyzed laccase and biomass p.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.1.40
- DOI:
- 10.4238/2015.December.1.40
Isolation and in silico functional analysis of MtATP6, a 6-kDa subunit of mitochondrial F1F0-ATP synthase, in response to abiotic stress
Authors: A.A. Moghadam S.M. Taghavi A. Niazi M. Djavaheri E. Ebrahimie
Mitochondrial F1F0-ATP synthase is a key enzymatic complex of energy metabolism that provides ATP for the cell. Subunits of this enzyme over-express under stress conditions. Little is known about the structure and regulatory mechanism of the F0 portion of this enzyme. We isolated the full-length coding sequence of the RMtATP6 gene from rice and wheat, and pa.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.October.4.3
- DOI:
- 10.4238/2012.October.4.3
In silico characterization of three two-component systems of Ehrlichia canis and evaluation of a natural plant-derived inhibitor
Authors: E.V. Santos G. Silva G.P. Cardozo T.A. Bitencourt S.C. Fran�§a A.L. Fachin M. Marins
Two-component signal transduction systems (TCS) are important elements in the interaction of endobacteria with host cells. They are basically composed of two proteins, an environmental signal sensor and a response regulator, which activate genes involved in a wide range of bacterial responses to their environment. We analyzed three sets of genes correspon.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.October.4.5
- DOI:
- 10.4238/2012.October.4.5
Molecular characterization of the pathogenic plant fungus Rhizoctonia solani (Ceratobasidiaceae) isolated from Egypt based on protein and PCR-RAPD profiles
Authors: M.A. Mahmoud S.A. Al-Sohaibani A.M.M. Abdelbacki M.R. Al-Othman A.R.M. Abd El- Aziz K.K. Kasem M.S. Mikhail K.K. Sabet M.R. Omar E.M. Hussein
Twenty-one isolates of Rhizoctonia solani were categorized into three anastomosis groups consisting of AG-4-HG-I (eight isolates), AG-2-2 (nine isolates) and AG-5 (four isolates). Their pathogenic capacities were tested on cotton cultivar Giza 86. Pre-emergence damping-off varied in response to the different isolates; however, the differences were not signif.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.October.4.6
- DOI:
- 10.4238/2012.October.4.6
A snapshot on prokaryotic diversity of the Solim�µes River basin (Amazon, Brazil)
Authors: D. Toyama, C.D. Santos-J�ºnior, L.T. Kishi, T.C.S. Oliveira, J.W. Garcia, H. Sarmento, F.P. Miranda and F. Henrique-Silva
The Amazon region has the largest hydrographic basin on the planet and âÂ?¨is well known for its huge biodiversity of plants and animals. However, âÂ?¨there is a lack of studies on aquatic microbial biodiversity in the âÂ?¨Solimões River, one of its main water courses. To investigate the âÂ?¨microbial biodiversi.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029567
- DOI:
- 10.4238/gmr16029567