Microbial Genetics
Knockdown expression of Apc11 leads to cell-cycle distribution reduction in G2/M phase
Authors: Y.-J. Shi K.-K. Huo
Anaphase-promoting complex/cyclosome (APC/C) is a key E3 ubiquitin ligase in cell division, which catalyses ubiquitination of cell-cycle regulators. Studying this complex could reveal important information regarding its application in cancer research and therapy. In this study, 4 synthesized small interfering RNAs (siRNAs) were transfected into HEK293T ce.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.August.24.6
- DOI:
- 10.4238/2012.August.24.6
Effects of miRNA-143 and the non-coding RNA MALAT1 on the pathogenesis and metastasis of HeLa cells
Authors: L. Zhang, H.E.X.D. Niyazi, H.R. Zhao, X.P. Cao, M.N.S. Abudula, W.J. Ye, S.A. Zhang, R.H.M. Yiming, Y. Zhang, W.P. Su, R. Chen, Y. Ouyang, N. Miao and Y.X. Bao
Cervical cancer is a common female malignancy of global dimensions. MicroRNAs (miRNAs) play crucial roles in the development, differentiation, proliferation, and apoptosis of tumors. The non-coding RNA MALAT1 participates in various physiological processes that are important for proper functioning of the body. Here, we analyze.. Read More»
- Genet. Mol. Res. 16(1):
- gmr16019269
- DOI:
- 10.4238/gmr16019269
Characterization and bacterial-binding activity of a novel C-type lectin from the red-spotted grouper, Epinephelus akaara
Authors: Z.W. Zhang X.F. Shi M. Qu S.X. Ding
Because of their specific binding to carbohydrates, lectins play a crucial role in pathogen recognition and clearance in vertebrate animals. Previously, only two types of collectins had been isolated from bony fish: mannan-binding lectin (MBL) and galactose-binding lectin (GalBL). We sequenced a novel collectin (designated EALec1) from the red-spotted gro.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.May.11.1
- DOI:
- 10.4238/2012.May.11.1
Identification and characterization of microsatellite markers from the tropical sea cucumber, Stichopus horrens (Selenka)
Authors: J.B. Shangguan, Z.B. Li, Y. Yuan and Y.S. Huang
Tropical commercial sea cucumber Stichopus horrens is extensively distributed throughout the tropical Indo-Pacific region, and wild stocks have been severely depleted over the past decade. In this study, we used the microsatellite enrichment library of S. horrens to identify and characterize 13 microsatellite loci, including 11 polymorphic loci and 2 mono.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.28.18
- DOI:
- 10.4238/2015.October.28.18
NEDD4-1 and PTEN expression in keloid scarring
Authors: P.F. Sang, H. Wang, M. Wang, C. Hu, J.S. Zhang, X.J. Li and F. Zhu
Keloid scarring remains a major problem in plastic surgery. The aim of this study was to determine the expression of the PTEN tumor suppressor and NEDD4-1 genes in keloid tissue and explore their effect on the formation of such scarring. Twenty keloid patients were enrolled in the study and underwent surgical removal of keloid tissue. No patient had recei.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.28.7
- DOI:
- 10.4238/2015.October.28.7
Expressed sequence tag-PCR markers for identification of alien barley chromosome 2H in wheat
Authors: M.J. Wang H.D. Zou Z.S. Lin Y. Wu X. Chen Y.P. Yuan
We developed EST-PCR markers specific to barley chromosome 2H, for the purpose of effectively tracing alien chromosomes or chromosome parts in the wheat genetic background. The target alien chromosome 2H confers high resistance to pre-harvest sprouting, which is a worldwide natural disaster in wheat. A total of 120 primer pairs were selected by combining .. Read More»
- Genet. Mol. Res. 11(3):
- 2012.September.25.13
- DOI:
- 10.4238/2012.September.25.13
Modification research on in wall of capillary copper tube with Norland optical adhesive 68 in a double stereo PCR microfluidic chip
Authors: J. Wu, F.J. Wang, C.Y. Wang, K.X. Yu, Y. Ma, T. Chen, Y.H. Li and Y. Zheng
In this study, a Norland optical adhesive 68 (NOA68) film, approximately 2.2 μm thick, was manufactured using ultraviolet solidified NOA68 in apparatus manufacturing film on the inwall of a capillary copper pipe, developed in our laboratory. The roughness of the inwall of capillary copper pipe was improved from Ra = 0.766 to 0.204 μm and the contact.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.28.21
- DOI:
- 10.4238/2015.October.28.21
Tangeritin inhibits adipogenesis by down-regulating C/EBP�±, C/EBP�², and PPAR�³ expression in 3T3-L1 fat cells
Authors: Y.F. He, F.Y. Liu and W.X. Zhang
The treatment of obese patients is a topic investigated by an increasing number of researchers. This study aimed to elucidate the possible inhibitory effect of tangeritin on the development and function of fat cells. 3T3-L1 fat cells were grown to confluence and subjected to different concentrations of tangeritin. The most effective tangeritin inhibition .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.28.26
- DOI:
- 10.4238/2015.October.28.26
Molecular phylogeny of Colletotrichum (Sordariomycetes: Glomerellaceae) inferred from multiple gene sequences
Authors: H. Chen, Y. Ma, W.F. Zhang, T. Ma and H.X. Wu
The genus Colletotrichum contains many important plant pathogens mainly distributed in tropical areas. Previous studies that utilized morphology or single genes have failed to resolve the phylogenetic relationships among the species. In this study, sequences of β-tubulin, 28S ribosomal DNA, and ITS region from nine species were analyzed separately an.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.28.27
- DOI:
- 10.4238/2015.October.28.27
Implicating the H63D polymorphism in the HFE gene in increased incidence of solid cancers: a meta-analysis
Authors: L.L. Shen, D.Y. Gu, T.T. Zhao, C.J. Tang, Y. Xu and J.F. Chen
A number of previous studies have demonstrated that the HFE H63D polymorphism is associated with increased risk of incidence multiple types of cancer, including colorectal cancer, breast cancer, liver cancer, pancreatic cancer, and gynecological malignant tumors. However, the clinical outcomes were inconsistent. Therefore, this meta-analysis was conducted.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.28.36
- DOI:
- 10.4238/2015.October.28.36