Microbial Genetics
Alpaca fiber growth is mediated by microRNA let-7b via down-regulation of target gene FGF5
Authors: T. Wang, Y. Zhang, H.D. Wang, Y. Shen, N. Liu, J. Cao, X.J. Yu, C.S. Dong and X.Y. He
MicroRNAs are very small endogenous RNA molecules that play a crucial role in an array of biological processes, including regulation of skin morphogenesis. The microRNA let-7b is thought to modulate animal hair growth, by binding target genes that encode growth factors. Fibroblast growth factor 5 (FGF5) has been previously reported to be involved in the i.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.28.38
- DOI:
- 10.4238/2015.October.28.38
Molecular cloning and characterization of the pseudorabies virus US1 gene
Authors: M.L. Li J.H. Chen Z.Y. Zhao K.J. Zhang Z. Li J. Li J.Y. Mai X.M. Zhu M.S. Cai
Using polymerase chain reaction, a 1050-bp sequence of the US1 gene was amplified from the pseudorabies virus (PRV) Becker strain genome; identification of the US1 gene was confirmed by further cloning and sequencing. Bioinformatics analysis indicated that the PRV US1 gene encodes a putative polypeptide with 349 amino acids. The encoded protein, designated P.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.January.22.7
- DOI:
- 10.4238/2013.January.22.7
Characterization of polymorphic microsatellite markers for the Neotropical leaf-frog Phyllomedusa burmeisteri and cross-species amplification
Authors: T.O. Brunes M.S. van de Vliet S. Lopes J. Alexandrino C.F.B. Haddad F. Sequeira
Twelve polymorphic tetranucleotide microsatellite loci were isolated and characterized for the leaf-frog Phyllomedusa burmeisteri, an endemic species of the Brazilian Atlantic forest. These loci were screened in 25 individuals from two populations of the Minas Gerais State (Carangola and Juiz de Fora). The number of alleles per locus ranged from 3 to 16 (.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.January.30.10
- DOI:
- 10.4238/2013.January.30.10
Snap bean recommendation based on different methods of phenotypic stability
Authors: C.D. Marinho G.A. Gravina L.C. Ara?ºjo S.N.C. Almeida A.T. Amaral J?ºnior R.F. Daher L.S.A. Gon?§alves
In order to recommend the best strains of snap beans from the Universidade Estadual do Norte Fluminense (UENF) breeding program, different methods of analysis of phenotypic stability were adopted to evaluate the performance of 14 lines (F9 and F10) of indeterminate growth habit, which were compared with 3 controls, namely, 2 commercial varieties (Feltrin .. Read More»
- Genet. Mol. Res. 12(1):
- 2013.January.30.11
- DOI:
- 10.4238/2013.January.30.11
Genetic divergence among Brazilian garlic cultivars based on morphological characters and AFLP markers
Authors: R.G.F. Morales J.T.V. Resende F.V. Resende C.A. Delatorre A.S.T. Figueiredo P.R. Da-Silva
Outside its centers of origin, garlic propagates only asexually. Since asexual reproduction leads to the absence of meiotic recombination, the main garlic cultivars available for cultivation have arisen from the accumulation of somatic mutations in early cultivars. Thus, it is common for a single clone to have different names in different regions. This st.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.February.4.1
- DOI:
- 10.4238/2013.February.4.1
Simple and inexpensive DNA extraction protocol for studying the bacterial composition of sludges used in microbial fuel cells
Authors: B. Canto-Canch?© M. Tzec-Sim?¡ J.I. V?¡zquez-Lor?a H. Espadas-?lvarez B.H. Ch?-Manzanero R. Rojas-Herrera R. Valdez-Ojeda L. Alzate-Gaviria
Bacteria oxidize organic matter and nutrients to produce electric energy in microbial fuel cells (MFC) - a technology of increasing importance because of its sustainability. To improve the performance of MFCs, it is necessary not only to gain a better understanding of MFC engineering designs, but also to improve the understanding of the composition of the mi.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.February.4.2
- DOI:
- 10.4238/2013.February.4.2
Effects of exogenous protease supplementation on endogenous trypsin activity and gene expression in broilers
Authors: L. Yuan, S.Q. Wang, Z.X. Wang, H. Zhu and K. Huang
Two hundred and forty one-day-old male broiler chickens (Ross-308) were fed corn-soybean basal diets with 0, 40, 80, or 160 mg/kg of an acid protease preparation from 1 to 42 days of age, in order to investigate the effects of an exogenous enzyme on growth performance, pancreatic trypsin activity and mRNA expression. Average daily gain (ADG) was greatest .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.28.25
- DOI:
- 10.4238/2015.October.28.25
Isolation and characterization of novel polymorphic microsatellite markers for Epinephelus akaara
Authors: Z.Z. Xie, L.Y. Zheng, L. Tang, Z.J. Tang, S.S. Li, Y. Zhang and H.R. Lin
In the present study, ten novel microsatellite markers were developed from an enriched-(CA)13 genomic library of Epinephelus akaara. The mean number of alleles per locus was 21.6, with a range of 12 to 33. Observed heterozygosity ranged from 0.767 to 0.967, and expected heterozygosity ranged from 0.831 to 0.975, with mean values of 0.877 and 0.923, respec.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.28.28
- DOI:
- 10.4238/2015.October.28.28
Structure and dynamics of the monomer of protein E of dengue virus type 2 with unprotonated histidine residues
Authors: L. Degr?¨ve C.A. Fuzo
The surface of the dengue virus is composed of 180 copies of a multifunctional envelope glycoprotein that acts at several stages of viral infection. When the virus is in the endosome, these glycoproteins undergo major conformational rearrangements owing to the protonation of histidine side chains. This protonation allows for the formation of trimers, thereby.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.February.7.5
- DOI:
- 10.4238/2013.February.7.5
VEGF, eNOS, and ABCB1 genetic polymorphisms may increase the risk of osteonecrosis of the femoral head
Authors: Z.C. Zhou, S.Z. Gu, J. Wu and Q.W. Liang
We investigated the associations between vascular endothelial growth factors (VEGF), endothelial nitric oxide synthase (eNOS), and ATP-binding cassette subfamily B member 1 transporter (ABCB1) polymorphisms and the risk of osteonecrosis of the femoral head (ONFH). Published studies were reviewed and analyzed based on predefined selection criteria. The str.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.28.31
- DOI:
- 10.4238/2015.October.28.31