Abstract

Modification research on in wall of capillary copper tube with Norland optical adhesive 68 in a double stereo PCR microfluidic chip

Author(s): J. Wu, F.J. Wang, C.Y. Wang, K.X. Yu, Y. Ma, T. Chen, Y.H. Li and Y. Zheng

In this study, a Norland optical adhesive 68 (NOA68) film, approximately 2.2 μm thick, was manufactured using ultraviolet solidified NOA68 in apparatus manufacturing film on the inwall of a capillary copper pipe, developed in our laboratory. The roughness of the inwall of capillary copper pipe was improved from Ra = 0.766 to 0.204 μm and the contact angle was improved from approximately 96° to 55°, increasing hydrophilicity. Polymerase chain reaction experiments indicated that the ratio of work pressure in the microfluidic chip before modification to that after modification was 2.71/1, indicating that the extension efficiency increased. Our results provide a basis for the construction of a microform chip based on function integration.

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