Medical Genetics
Expression of PCA3 and PSA genes as a biomarker for differential diagnosis of nodular hyperplasia and prostate cancer
Authors: F. Fonseca Coelho, F. Loli Guimar�£es, W.L. Ribeiro Cabral, P.G. Oliveira Salles, E. Cueva Mateo, L. Mendes Nogueira e Nogueira, C.E. Corradi Fonseca and K. Braga Gomes
We evaluated the expression of the PCA3 gene in urine from patients with nodular hyperplasia/benign prostatic hyperplasia (PNH) or adenocarcinoma type prostate cancer (PCa).The study included 59 men: 22 with PCa, 26 with PNH, and 11 with no alterations (controls). Patients’ urine was collected following prostatic massage and quantified by quantitati.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.28.13
- DOI:
- 10.4238/2015.October.28.13
Proteomic analysis of susceptibility in intestinal stromal tumors
Authors: Y.C. Yang, X. Cai, S.Y. Zhang, Z.L. Jia, T.B. Chen, L.R. Liu, J. Liu and M.C. Zhao
We analyzed the susceptibility of intestinal stromal tumors using cell culture and proteomics. Human SGC7901 gastric cells were selected and divided into a blank control group (untransfected SGC7901 cells), a negative control group [SGC7901 cells transfected with negative interference control-small interfering RNA (siRNA)], and a COOH-terminus tensin-like.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.28.16
- DOI:
- 10.4238/2015.October.28.16
Relationship between serum leptin levels and non-small cell lung carcinoma: a meta-analysis
Authors: J. Du, J.C. Han, Y.J. Zhang, G.B. Qi, Y. Zhang and H.B. Li
In this study, we analyzed the association between serum leptin levels and non-small cell lung carcinoma (NSCLC). By examining English and Chinese databases, we identified potential relevant studies for statistical analysis. Human-associated case-control studies evaluating the association between serum leptin levels and NSCLC according to the random-effec.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.28.32
- DOI:
- 10.4238/2015.October.28.32
Lack of mutations of G4.5 in three families from China with noncompaction of the ventricular myocardium
Authors: Q. Liu X.F. Qi F. Ye J. Yao J. Xu
To find the underlying cause of noncompaction of the ventricular myocardium (NVM), three Chinese families with probands who presented this problem were studied. After the family members were evaluated by echocardiography, the gene G4.5 (taffazin) was scanned by sequencing. Although X-linked inheritance could not be ruled out, NVM were thought to have a va.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.January.22.3
- DOI:
- 10.4238/2013.January.22.3
Cloning, characterization and expression analysis of coagulation factor II gene in grass carp (Ctenopharyngodon idella)
Authors: B.H. Xu, K.J. Chen, Y.B. Yao, Q.L. Liu, T.Y. Xiao, J.M. Su and H.Z. Peng
Here, we characterized the structure and function of the coagulation factor II (FII) gene in grass carp and determined its role in coagulation mechanisms. The FII gene EST was obtained using a constructed splenic transcriptome database; the full-length FII gene sequence was obtained by 3' and 5' RACE. The open reading frame (ORF) of FII was cloned and the.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.28.39
- DOI:
- 10.4238/2015.October.28.39
Methylation profile of SNRPN gene and its correlation with weight and chronological age
Authors: N.V. Carobin, F.V.M. Rubatino, M.L. Freitas, V.T. de Oliveira, R.X. Pietra, A.A. Bosco and F.S. Jehee
Genomic imprinting is an important epigenetic phenomenon, wherein genes or gene clusters are marked by DNA methylation during gametogenesis. This plays a major role in several functions of normal cells, including cell differentiation, X chromosome inactivation, and the maintenance of chromatin structure, in mammalian development. The aim of this study was.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.28.41
- DOI:
- 10.4238/2015.October.28.41
Drought-tolerant rice germplasm developed from an Oryza officinalis transformation-competent artificial chromosome clone
Authors: R. Liu, H.H. Zhang, Z.X. Chen, M.Q. Shahid, X.L. Fu and X.D. Liu
Oryza officinalis has proven to be a natural gene reservoir for the improvement of domesticated rice as it carries many desirable traits; however, the transfer of elite genes to cultivated rice by conventional hybridization has been a challenge for rice breeders. In this study, the conserved sequence of plant stress-related NAC transcription factors was s.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.28.29
- DOI:
- 10.4238/2015.October.28.29
Screening of polymorphisms located in the FGF20 and TMEM175 genes in North Chinese Parkinson�s disease patients
Authors: C.C. Jing, X.G. Luo, H.G. Cui, F.R. Li, P. Li, E.Z. Jiang, Y. Ren and H. Pang
Genome-wide association studies have reported numerous susceptibility loci for Parkinson’s disease (PD). However, there have been few replication studies examining these loci in northern Chinese populations. To evaluate the relationships among 3 polymorphic markers located in the fibroblast growth factor 20 and transmembrane protein 175 genes and th.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.28.30
- DOI:
- 10.4238/2015.October.28.30
Identification and characterization of the duck enteritis virus (DEV) US2 gene
Authors: J. Gao, A.C. Cheng, M.S. Wang, R.Y. Jia, D.K. Zhu, S. Chen, M.F. Liu, F. Liu, Q. Yang, K.F. Sun and X.Y. Chen
The US2 protein has been reported to contribute to duck enteritis virus (DEV) infection; however, its kinetics and localization during infection, and whether it is a component of virion, have not been previously reported. To elucidate the function of DEV US2, US2 was amplified by polymerase chain reaction (PCR) and inserted into pET-32a(+); this was expre.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.28.40
- DOI:
- 10.4238/2015.October.28.40
Conjunctiva reconstruction by induced differentiation of human amniotic epithelial cells
Authors: S.P. Yang, X.Z. Yang and G.P. Cao
In this study, we aimed to investigate the feasibility of directed differentiation of human amniotic epithelial cells into conjunctival epithelium under specific conditions as well as of constructing tissue-engineered conjunctiva for ocular surface reconstruction. Human amniotic epithelial cells were cultured with induced denuded conjunctival matrix and c.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.29.2
- DOI:
- 10.4238/2015.October.29.2